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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
hydrolysis of aliphatic amide
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
ping-pong mechanism, substitution mechanism
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
hydrolysis of ester and aromatic amide
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
stereospecific hydrolysis of 2-arylpropionamides
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
catalytic role of an arginine dyad, the catalytic motif is revealed in the model structure of amide hydrolyzing antibody 312D6, the antibody induces catalysis by transition-state mimicry and torsional activation, and accelerates the hydrolysis of amides by a factor of 1000, overview
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
the enzyme contains a nitrilase catalytic triad at the bottom of a 13-A deep, funnel-shaped pocket, accessible from the solvent through a narrow neck with 3-A diameter
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a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
amino acid residues Ser171, Ser195 and Lys96 are essential for catalytic activity, molecular mechanism of substrate recognition, overview
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
hydrolysis of ester and aromatic amide
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(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
(R)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(R)-2,2-dimethylcyclopropane hydroxamic acid + NH3
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
(R)-lactamide + H2O
lactate + NH3
about 30% of the activity with (S)-lactamide
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?
(R,S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
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enantioselective for the S-enantiomer
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?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + NH3
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the enzyme is highly S-stereoselective for 2-phenylpropionamide, and the racemic amide is converted to the corresponding S-acid with an enantiomeric excess of over 95% at 50% conversion of the substrate
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?
(R,S)-ketoprofen amide + H2O
(S)-ketoprofen + NH3
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the enzyme hydrolyzes S-ketoprofen amide to its corresponding acid in an absolutely enantioselective manner
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?
(R/S)-2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + (S)-2,2-dimethylcyclopropanecarboxyamide
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?
(R/S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + (R)-piperazine-2-carboxamide
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?
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
(S)-(-)-2-hydroxy-2-(trifluoromethyl)-butanamide + H2O
(R)-(+)-2-hydroxy-2-(trifluoromethyl)-butanoic acid + NH3
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stereospecific reaction
enantiomerically pure product
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?
(S)-(-)-3,3,3-trifluoro-2-amino-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-amino-2-methylpropanoic acid + NH3
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stereospecific reaction
enantiomerically pure product
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?
(S)-(-)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
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stereospecific reaction
enantiomerically pure product
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?
(S)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(S)-2,2-dimethylcyclopropane hydroxamic acid + NH3
(S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
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ketoprofen amide, R-amide is not converted
ketoprofen
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?
(S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionate + NH3
no activity with (R)-2-phenylpropionamide
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?
(S)-ibuprofen amide + H2O
(S)-ibuprofen + NH3
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?
(S)-lactamide + H2O
lactate + NH3
1-naphthalene-acetamide + H2O
1-naphthaleneacetic acid + NH3
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about 60% of the activity with indole-3-acetamide
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?
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
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?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
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?
2',6'-dimethylacetanilide + H2O
2,6-dimethylaniline + acetate
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?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
2-arachidonoylglycerol + H2O
?
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
2-chloroacetamide + H2O
2-chloroacetate + NH3
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?
2-chloropropionamide + H2O
2-chloropropionate + NH3
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
2-nitroacetanilide
2-nitrophenylamine + acetate
2-thiophenecarboxamide + H2O
2-thiophenecarboxylate + NH3
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?
2-toluamide + H2O
2-toluic acid + NH3
2-tolylamide + H2O
? + NH3
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?
3',4'-dichloropropionanilide + H2O
3,4-dichloroaniline + propanoate
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?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
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?
3-hydroxymyristoyl lipid A + H2O
?
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3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
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?
3-indolacetamide + H2O
3-indolacetate + NH3
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?
3-indolylacetamide + H2O
3-indolylacetic acid + NH3
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?
3-phenyl-propionamide + H2O
3-phenyl-propionate + NH3
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?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
3-toluamide + H2O
3-toluic acid + NH3
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?
3-tolylamide + H2O
? + NH3
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?
4'-methylacetanilide + H2O
4-methylaniline + acetate
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?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyrate + NH3
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substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
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?
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyric acid + NH3
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?
4-guanidinobutyramide + H2O
?
4-hydroxybenzamide + H2O
4-hydroxybenzoate + NH3
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?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
4-nitroacetanilide + H2O
4-nitroaniline + acetate
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
4-nitrobenzamide + H2O
4-nitrobenzoate + NH3
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?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-toluamide + H2O
4-toluic acid + NH3
4-tolylamide + H2O
? + NH3
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?
5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
glutarate + 4-nitroaniline
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?
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid + H2O
2-nitrobenzoate + adipate
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?
acetamide + H2O
acetate + NH3
acetamide + H2O
acetic acid + NH3
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
acetanilide + H2O
acetate + aniline
acetate-prelabeled chicken histones + H2O
deacetylated chicken histones + acetate
acetohydrazide + H2O
hydrazine + acetate
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?
acetohydroxamate + H2O
hydroxylamine + acetate
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?
acetylcadaverine + H2O
acetate + cadaverine
acetylputrescine + H2O
acetate + putrescine
acetylspermidine + H2O
acetate + spermidine
acrylamide + H2O
acrylate + NH3
acrylamide + H2O
acrylic acid + NH3
acrylamide + H2O
prop-2-enoic acid + NH3
acrylamide + hydroxylamine
acrylhydroxamic acid + NH3
acrylamide + hydroxylamine
acryloylhydroxamic acid + NH3
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?
acylamide + H2O
carboxylic acid + NH3
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?
adipoamide + H2O
adipic acid + NH3
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?
adipyl-4-nitro-aniline + H2O
?
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?
anandamide + H2O
arachidonic acid + ethanolamine
anthranilamide + H2O
anthranilate + NH3
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?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
benzamide
benzoic acid + NH3
Blastobacter sp.
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?
benzamide + H2O
benzoate + NH3
benzamide + H2O
benzoic acid + NH3
benzamide + hydroxylamine
benzoylhydroxamic acid + NH3
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
butyramide + H2O
butyrate + NH3
butyramide + H2O
butyric acid + NH3
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?
butyramide + H2O
n-butyrate + NH3
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?
butyramide + hydroxylamine
butyrylhydroxamic acid + NH3
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
carboxylic acid amide + H2O
carboxylic acid + NH3
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
cinnamamide + H2O
cinnamic acid + NH3
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide + H2O
cis-(1S,4R)-(4-(hydroxymethyl)cyclopent-2-enyl)amine + acetate
used as enzymatic assay
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?
crotonic acid + NH3
crotonamide + H2O
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5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide
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r
cyanoacetamide + H2O
cyanoacetate + NH3
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low activity
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?
cyclohexanamide + H2O
cyclohexanoate + NH3
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?
cyclohexanoamide + H2O
cyclohexanoic acid + NH3
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?
D-lactamide + H2O
D-lactate + NH3
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17% of the activity with acetamide, D-selectivity towards lactamide
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?
D-lactamide + hydroxylamine
?
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?
D-Val-L-Leu-L-Arg 4-nitroanilide + H2O
D-Val-L-Leu-L-Arg + 4-nitroaniline
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?
diacetamide + H2O
?
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14% of the activity with acetamide
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?
DL-lactamide + H2O
DL-lactate + NH3
DL-methioninamide + H2O
DL-methionine + NH3
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?
DL-tryptophanamide + H2O
DL-tryptophan + NH3
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?
ethyl acetate + H2O
acetic acid + ethanol
ethyl acetate + H2O
ethanol + acetate
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?
ethyl acrylate + H2O
acrylate + ethanol
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?
ethyl propionate + H2O
ethanol + propionate
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?
fluoroacetamide + H2O
fluoroacetate + NH3
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165% of the activity with acetamide
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?
formamide + H2O
formate + NH3
glutaramic acid + H2O
glutaric acid + NH3
Blastobacter sp.
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?
glutaryl-2-naphthylamine + H2O
?
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?
glutaryl-aniline + H2O
?
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?
glutaryl-nitro-5-amino benzoic acid + H2O
?
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?
hexanamide + H2O
hexanoate + NH3
hexanamide + hydroxylamine
hexanoylhydroxamic acid + NH3
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?
hexanoamide + H2O
hexanoic acid + NH3
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?
hydroxylamine + acylamide
acylhydroxamate + NH3
3.5.1.4 amidase
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