Literature summary for 4.2.1.84 extracted from

Singh, R.; Pandey, D.; Dhariwal, S.; Sood, P.; Chand, D.
Bioconversion of acrylonitrile using nitrile hydratase activity of Bacillus sp. APB-6 (2018), 3 Biotech, 8, 225 .

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Application

Application	Comment	Organism
analysis	the enzyme is used in production of acrylamide from acrylonitrile. For maximum production of Co2+ containing nitrile hydratase, is cultured in the medium containing lactose (18.0 g/l), peptone (1.0 g/l), yeast extract (2.0 g/l), MgSO4 (0.5 g/l), K2HPO4 (0.6 g/l), urea (9.0 g/l), and CoCl2 (0.01 g/l), pH 7.0, and incubated at 35°C for 24 h in an incubator shaker (160 rpm). Nitrile hydratase exhibits relatively high specificity for aliphatic nitriles. Free cells are immobilized using 2% (w/v) agar solution to enhance enzyme stability and reusability in repetitive cycles of acrylamide production. Under optimized conditions, nearly complete bioconversion of acrylonitrile is achieved with a fair recovery of 85% using free and immobilized cells equivalent to 500 mg/dry cell weight/l	Bacillus sp. APB-6

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. APB-6	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acrylonitrile + H2O	-	Bacillus sp. APB-6	acrylamide	-	?

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Release 2023.1 (January 2023)