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(1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbonitrile + H2O
(1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbamide
(1R,3R)-3-phenylcyclopropanecarbonitrile + H2O
(1R,3R)-3-phenylcyclopropanecarbamide
(1R,3S)-3-phenylcyclopropanecarbonitrile + H2O
(1R,3S)-3-phenylcyclopropanecarbamide
(1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbonitrile + H2O
(1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbamide
(25R)-3beta-hydroxycholest-5-en-27-oate
?
-
-
-
-
?
(2R)-2-hydroxy-3-methylbutanenitrile + H2O
(2R)-2-hydroxy-3-methylbutanamide
-
-
-
-
?
(2R)-2-hydroxybut-3-enenitrile + H2O
(2R)-2-hydroxybut-3-enamide
-
-
-
-
?
(2R)-2-hydroxybutanenitrile + H2O
(2R)-2-hydroxybutanamide
-
-
-
-
?
(2R)-2-hydroxyhexanenitrile + H2O
(2R)-2-hydroxyhexanamide
-
-
-
-
?
(2R)-2-hydroxypentanenitrile + H2O
(2R)-2-hydroxypentanamide
-
-
-
-
?
(R)-2-chloromandelonitrile + H2O
(R)-2-chloromandelamide
at 100% the rate of 2-hydroxy-4-phenylbutyronitrile
-
-
?
(R)-mandelonitrile + H2O
(R)-mandelamide
(R,S)-2-(4-nitrophenyl)-propionitrile + H2O
?
-
39% conversion
-
-
?
(R,S)-2-bromopropionitrile + H2O
?
-
47% conversion
-
-
?
(R,S)-2-chloropropionitrile + H2O
?
-
48% conversion
-
-
?
(R,S)-2-phenylbutyronitrile + H2O
?
-
51% conversion
-
-
?
(R,S)-2-phenylpropionitrile + H2O
?
-
43% conversion
-
-
?
(R,S)-3-oxo-2-phenylbutyronitrile + H2O
?
-
43% conversion
-
-
?
(S)-3-benzoyloxypentanedinitrile + H2O
3-amino-1-(2-amino-2-oxoethyl)-3-oxopropyl benzoate
-
substrate conversion: 38.5%, enantiomeric excess: 68.2
-
-
r
(S)-mandelonitrile + H2O
(S)-mandelamide
1,1,3,3,-tetramethylbutylisonitrile + H2O
1,1,3,3,-tetramethylisobutylamide
1-(4-bromo-phenyl)-aziridine-2-carbonitrile + H2O
1-(4-bromophenyl)aziridine-2-carboxamide
-
-
-
-
r
1-(4-methoxy-phenyl)-aziridine-2-carbonitrile + H2O
1-(4-methoxyphenyl)aziridine-2-carboxamide
-
-
-
-
r
1-cyanocyclohexaneacetonitrile + H2O
1-cyanocyclohexaneacetamide
1-naphthylnitrile + H2O
1-naphthylamide
17alpha-cyanomethyl-17beta-hydroxy-estra-4,9-dien-3-one + H2O
17alpha-acetamido-estra-1,3,5(10),9(11)-tetraene-3,17beta-diol
-
the steroidal group is metabolized very slowly
-
?
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(R)-(4-chloro-phenyl)-3-methyl-butyramide
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
2,2-dimethylcyclopropanecarbonitrile + H2O
2,2-dimethylcyclopropanecarbamide
2,2-dimethylcyclopropanecarbonitrile + H2O
2,2-dimethylcyclopropanecarboxamide
2,2-dimethylcyclopropanecarbonitrile + H2O
?
2,3,4,5,6-pentafluorobenzonitrile + H2O
2,3,4,5,6-pentafluorobenzamide
-
-
-
-
?
2,3-dihydro-benzo(1,4)dioxine-2-carbonitrile + H2O
2,3-dihydro-1,4-benzodioxine-2-carboxamide
-
substrate conversion: 45.1%, enantiomeric excess: 0
-
-
r
2,6-dichlorobenzamide + H2O
2,6-dichlorobenzoic acid
2,6-difluorobenzonitrile + H2O
2,6-difluorobenzamide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
2-aminopropionitrile + H2O
2-aminopropionic acid amide
-
90% of the activity with propionitrile
-
-
?
2-bromobenzonitrile + H2O
2-bromobenzamide
-
-
-
-
ir
2-chlorobenzaldehyde + HCN + H2O
(R)-2-chloromandelonitrile
-
-
90% conversion to alpha-hydroxy nitrile
-
?
2-chlorobenzonitrile + H2O
2-chlorobenzamide
-
-
-
-
ir
2-cyanobenzamide + H2O
benzene-1,2-dicarboxamide
2-cyanopyridine + H2O
pyridine-2-carbamide
2-fluorobenzaldehyde + HCN + H2O
? + H2O
-
-
100% conversion to alpha-hydroxy nitrile
-
?
2-fluorobenzonitrile + H2O
2-fluorobenzamide
-
-
-
-
ir
2-furonitrile + H2O
2-furoamide
2-hydroxy-4-phenylbutanenitrile + H2O
?
-
-
-
?
2-hydroxy-4-phenylbutyronitrile + H2O
2-hydroxy-4-phenylbutyramide
-
-
-
?
2-hydroxymethyl-3-phenyl-propionitrile + H2O
2-benzyl-3-hydroxypropanamide
-
-
-
-
r
2-hydroxypropionitrile + H2O
2-hydroxypropionic acid amide
2-methoxybenzonitrile + H2O
2-methoxybenzamide
-
-
-
-
ir
2-methoxymethyl-3-phenyl-propionitrile + H2O
2-benzyl-3-methoxypropanamide
-
-
-
-
r
2-methyl-3-butenenitrile + H2O
?
2-methylbenzonitrile + H2O
2-methylbenzamide
-
-
-
-
ir
2-naphthylacetonitrile + H2O
2-naphthylacetamide
2-nitro-5-thiocyanato-benzoic acid + H2O
?
-
-
-
-
?
2-nitrobenzaldehyde + HCN + H2O
? + H2O
-
-
20% conversion to alpha-hydroxy nitrile
-
?
2-phenylacetonitrile + H2O
2-phenylpropionamide
-
-
-
-
?
2-phenylbutyronitrile + H2O
2-phenylbutyramide
-
used as well as phenylacetonitrile
-
-
?
2-phenylglycinonitrile + H2O
aminoacetamide
-
used as well as phenylacetonitrile
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
3,4,5-trimethoxybenzonitrile + H2O
3,4,5-trimethoxybenzamide
3,4-dimethoxybenzonitrile + H2O
3,4-dimethoxybenzaldehyde
3,4-dimethoxybenzonitrile + H2O
3,4-dimethoxybenzaldehyde + HCN
3-(1-cyanoethyl)benzoic acid + H2O
?
3-(trifluoromethyl)benzonitrile + H2O
3-(trifluoromethyl)benzamide
-
5% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-(trifluoromethyl)pyridine-4-carbonitrile + H2O
3-(trifluoromethyl)pyridine-4-carboxamide
-
-
-
-
ir
3-allyloxy-4-phenyl-butyronitrile + H2O
4-phenyl-3-(prop-2-en-1-yloxy)butanamide
-
-
-
-
r
3-aminopropionitrile + H2O
3-aminopropionic acid amide
-
2.7% of the activity with propionitrile
-
-
?
3-benzoyloxyglutaronitrile + H2O
(S)-3-benzoyloxy-4-cyanobutyramide
3-benzyloxy-3-vinyl-propionitrile + H2O
3-(benzyloxy)pent-4-enamide
-
-
-
-
r
3-benzyloxy-pentanonitrile + H2O
3-(benzyloxy)pentanamide
-
-
-
-
r
3-benzyloxyglutaronitrile + H2O
3-benzyloxy-4-cyanobutyramide
3-bromobenzonitrile + H2O
3-bromobenzamide
-
5% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-chlorobenzaldehyde + HCN + H2O
? + H2O
-
-
100% conversion to alpha-hydroxy nitrile
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzamide
3-cyanopyridine + H2O
nicotinamide
3-cyanopyridine + H2O
pyridine-3-carbamide
3-cyanopyridine + H2O
pyridine-3-carboxamide
3-fluorobenzonitrile + H2O
3-fluorobenzamide
-
above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-hydroxy-3-phenylpropionitrile + H2O
3-hydroxy-3-phenylpropionamide
-
-
-
-
?
3-hydroxybenzonitrile + H2O
3-hydroxybenzamide
3-hydroxybutryronitrile + H2O
3-hydroxybutyramide
3-hydroxypropionitrile + H2O
3-hydroxypropanamide
-
35% of the activity with propionitrile
-
-
?
3-hydroxypropionitrile + H2O
3-hydroxypropionamide
3-hydroxyvaleronitrile + H2O
3-hydroxyvaleramide
3-methoxybenzonitrile + H2O
3-methoxybenzamide
-
3% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-methylbenzonitrile + H2O
3-methylbenzamide
3-phenoxymandelonitrile + H2O
3-phenoxymandelamine
at 10% the rate of 2-hydroxy-4-phenylbutyronitrile
-
-
?
3-phenylpropanenitrile + H2O
3-phenylpropanamide
3-phenylpropionitrile + H2O
3-phenylpropionamide
-
used as well as phenylacetonitrile
-
-
?
4-(trifluoromethyl)benzonitrile + H2O
4-(trifluoromethyl)benzamide
4-acetylbenzonitrile + H2O
4-acetylbenzamide
4-aminobenzonitrile + H2O
4-aminobenzamide
4-bromobenzonitrile + H2O
4-bromobenzamide
-
-
-
-
ir
4-bromobenzonitrile + H2O
4-bromobenzonitrile
-
above 99% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
4-chloro-3-hydroxybutanenitrile + H2O
?
-
-
-
?
4-chloro-3-hydroxybutyronitrile + H2O
4-chloro-3-hydroxybutyramide
-
the following reaction by an amidase leads to the correspondend carboxylic acid
-
-
?
4-chlorobenzaldehyde + HCN + H2O
? + H2O
-
-
100% conversion to alpha-hydroxy nitrile
-
?
4-chlorobenzonitrile + H2O
4-chlorbenzamide
-
-
-
-
ir
4-chlorobenzonitrile + H2O
4-chlorobenzamide
4-chlorobutyronitrile + H2O
4-chlorobutyramide
-
-
-
?
4-cyanobenzaldehyde + HCN + H2O
? + H2O
-
-
100% conversion to alpha-hydroxy nitrile
-
?
4-cyanobenzamide + H2O
benzene-1,4-dicarboxamide
4-cyanobenzoic acid + H2O
4-(aminocarbonyl)benzoic acid
-
-
-
-
?
4-cyanopyridine + H2O
isonicotinamide
4-cyanopyridine + H2O
pyridine-4-carbamide
4-fluorobenzonitrile + H2O
4-fluorbenzamide
-
-
-
-
ir
4-fluorobenzonitrile + H2O
4-fluorobenzamide
-
above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
4-hydroxybenzaldehyde + HCN + H2O
? + H2O
-
-
55% conversion to alpha-hydroxy nitrile
-
?
4-hydroxybenzonitrile + H2O
4-hydroxybenzamide
-
-
-
-
?
4-hydroxybenzonitrile + H2O
4-hydroxybenzoic acid amide
4-hydroxyphenylacetonitrile + H2O
4-hydroxyphenylacetamide
4-methoxybenzonitrile + H2O
4-methoxybenzamide
4-methylbenzaldehyde + HCN + H2O
? + H2O
-
-
51% conversion to alpha-hydroxy nitrile
-
?
4-methylbenzonitrile + H2O
4-methylbenzamide
4-methylmandelonitrile + H2O
4-methylmandelamine
at 60% the rate of 2-hydroxy-4-phenylbutyronitrile
-
-
?
4-nitrobenzaldehyde + HCN + H2O
? + H2O
-
-
100% conversion to alpha-hydroxy nitrile
-
?
5-cyanovaleramide
adiponitrile + H2O
5-cyanovaleric acid + H2O
6-amino-6-oxohexanoic acid
-
NilFe and NilCo
-
-
r
5-hydroxymethyl-2-furonitrile + H2O
5-hydroxymethyl-2-furamide
acetamiprid + H2O
(1E)-N'-carbamoyl-N-[(6-chloropyridin-3-yl)methyl]-N-methylethanimidamide
hydration at 22.41% compared to the activity with thiacloprid
-
-
?
acetonitrile + H2O
acetamide
acrylamide + H2O
acrylonitrile
acrylonitrile + H2O
2-propenoic acid amide
acrylonitrile + H2O
acrylamide
adiponitrile + H2O
5-cyanovaleramide
adiponitrile + H2O
adipamide
adiponitrile + H2O
adipic acid amide
aeroplysinin-1 + H2O
verongiaquinol
-
high substrate specificity towards the physiological substrate aeroplysinin-1
-
-
?
alpha-methylbenzyl cyanide + H2O
2-phenylpropanamide
an aliphatic amide
a nitrile + H2O
benzaldehyde + HCN
? + H2O
-
-
95% conversion to alpha-hydroxy nitrile
-
?
benzeneacetonitrile + H2O
?
very low activity
-
-
?
benzonitrile + H2O
benzamide
benzonitrile + H2O
benzoic acid amide
benzonitrile + hydroxylamine + H2O
benzohydroxamic acid + NH3
benzyl cyanide + H2O
2-phenylacetamide
85% of the activity compared to 3-cyanopyridine
-
-
?
benzylcyanide + H2O
2-phenylacetamide
-
-
-
-
r
butyronitrile + H2O
?
-
-
-
?
butyronitrile + H2O
butyramide
butyronitrile + H2O
butyric acid amide
-
best substrate
-
-
?
chloroacetonitrile + H2O
chloroacetamide
chloroxynil amide + H2O
?
crotononitrile + H2O
(E)-2-butenoic acid amide
cyanamide + H2O
urea
-
-
-
?
cyanoacetamide
malononitrile + H2O
-
-
-
r
cyanopyrazine + H2O
pyrazincarbamide
-
-
-
-
?
cyanovaleramide
valerodinitrile + H2O
cyanovaleric acid + H2O
?
cyclopropylcyanide + H2O
?
-
-
-
-
?
dichlobenil acid + H2O
?
-
-
-
-
?
dichlobenil amide + H2O
?
ethyl 2-cyanobenzoate + H2O
ethyl 2-carbamoylbenzoate
-
-
-
-
ir
ethyl 3-cyanobenzoate + H2O
ethyl 3-carbamoylbenzoate
-
no conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
ethyl 4-cyanobenzoate + H2O
ethyl 4-carbamoylbenzoate
ethylene cyanhydrine + H2O
?
furan-2-carbonitrile + H2O
furan-2-carboxamide
glutaronitrile + H2O
?
-
38% of the activity with propionitrile
-
-
?
glycolonitrile + H2O
glycolamide
-
yield 63%
-
?
hexanedinitrile + H2O
?
hydration at 0.43% compared to the activity with thiacloprid
-
-
?
hydroxyacetonitrile + H2O
hydroxyacetamide
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
indole-3-acetonitrile + H2O
(indole-3-yl)acetamide
indole-3-acetonitrile + H2O
indole-3-acetamide
indole-3-nitrile + H2O
indole-3-acetamide
isobutyronitrile + H2O
?
-
-
-
?
isobutyronitrile + H2O
isobutyramide
isobutyronitrile + H2O
isobutyric acid amide
isovaleronitrile + H2O
isovaleric acid amide
malonitrile + H2O
?
-
44% of the activity with propionitrile
-
-
?
malononitrile + H2O
cyanoacetamide
the use of the wild-type enzyme leads to malonamide formation with 97.3% malononitrile conversion. Variants Y68T and W72Y show a drastic change in regiospecificity by producing mainly the omega-cyanocarboxamide, cyanoacetamide, at a relatively low malononitrile conversion. Mutant enzyme Y68T/W72Y produces 97.1% omega-cyanocarboxamide
-
-
r
malononitrile + H2O
malonamide
the use of the wild-type enzyme leads to malonamide formation with 97.3% malononitrile conversion. Variants Y68T and W72Y show a drastic change in regiospecificity by producing mainly the omega-cyanocarboxamide, cyanoacetamide, at a relatively low malononitrile conversion. Mutant enzyme Y68T/W72Y produces 97.1% omega-cyanocarboxamide
-
-
?
methacrylonitrile + H2O
?
-
-
-
?
methacrylonitrile + H2O
methacrylamide
methacrylonitrile + H2O
methacrylic acid amide
-
-
-
-
?
methacrylonitrile + H2O
methylacrylamide
methacrylonitrile + H2O
methylacrylic acid amide
methoxyacetonitrile + H2O
?
-
-
-
-
?
methyl 4-cyanobenzoate + H2O
methyl 4-carbamoylbenzoate
n-butyronitrile + H2O
n-butyramide
-
-
-
-
r
n-butyronitrile + H2O
n-butyric acid amide
n-capronitrile + H2O
n-hexanoic acid amide
N-phenylglycinenitrile + H2O
?
-
-
-
-
?
n-valeronitrile + H2O
n-valeramide
nicotinonitrile + H2O
nicotinamide
o-chlorobenzonitrile + H2O
o-chlorobenzamide
p-aminobenzonitrile + H2O
p-aminobenzamide
-
conversion rate: 8.98%
-
-
?
p-chlorobenzonitrile + H2O
p-chlorobenzamide
-
conversion rate: 93.1%
-
-
?
p-hydroxybenzylcyanide + H2O
2-(4-hydroxyphenyl)acetamide
-
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetic acid + NH3
-
-
-
?
phenylacetonitrile + H2O
2-phenylacetamide
phenylacetonitrile + H2O
phenylacetamide
phthalodinitrile + H2O
2-cyanobenzamide
the wild-type enzyme forms 100% phthalamide from phthalodinitrile. The mutant enzymes Y68T and W72Y result in a higher 2-cyanobenzamide formation than their parent enzyme. Mutant enzyme Y68T/W72Y produces 100% 2-cyanobenzamide from phthalodinitrile
-
-
?
phthalodinitrile + H2O
phthalamide
the wild-type enzyme forms 100% phthalamide from phthalodinitrile. The mutant enzymes Y68T and W72Y result in a higher 2-cyanobenzamide formation than their parent enzyme. Mutant enzyme Y68T/W72Y produces 100% 2-cyanobenzamide from phthalodinitrile
-
-
?
phthalonitrile + H2O
2-cyanobenzamide
-
lowest activity
-
-
?
phthalonitrile + H2O
?
-
-
-
-
?
pivalonitrile + H2O
2,2-dimethylpropionic acid amide
propionitrile + H2O
propionamide
propionitrile + H2O
propionic acid amide
pyridine-2-carbonitrile + H2O
pyridine-2-carboxamide
pyridine-4-carbonitrile + H2O
pyridine-4-carboxamide
terephthalonitrile + H2O
4-cyanobenzamide
the wild-type enzyme prefers terephthalonitrile to catalyze mainly into terephthalamide (84.3%) with a conversion up to 99.2 %. Variants Y68T and W72Y show a change in regiospecificity by producing mainly the 4-cyanobenzamide. Mutant enzyme Y68T/W72Y produces 98.2% 4-cyanobenzamide from terephthalonitrile
-
-
?
terephthalonitrile + H2O
terephthalamide
the wild-type enzyme prefers terephthalonitrile to catalyze mainly into terephthalamide (84.3%) with a conversion up to 99.2 %. Variants Y68T and W72Y show a change in regiospecificity by producing mainly the 4-cyanobenzamide. Mutant enzyme Y68T/W72Y produces 98.2% 4-cyanobenzamide from terephthalonitrile
-
-
?
tert-butylisonitrile + H2O
?
-
-
-
?
tert-butylisonitrile + H2O
tert-butyl amide
thiacloprid + H2O
1-[(2Z)-3-[(6-chloropyridin-3-yl)methyl]-1,3-thiazolidin-2-ylidene]urea
-
-
-
?
thiophen-2-ylacetonitrile + H2O
2-(thiophen-2-yl)acetamide
thiophene-2-carbonitrile + H2O
thiophen-2-carboxamide
-
-
-
-
ir
thiophene-2-carbonitrile + H2O
thiophene-2-carboxamide
-
99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
toyocamycin + H2O
toyocamycin acid amide
-
-
-
?
trans-4-cyanocyclohexane-1-carboxylic acid + H2O
4-(aminocarbonyl)cyclohexanecarboxylic acid
trans-cinnamonitrile + H2O
trans-cinnamide
-
-
-
-
?
valeronitrile + H2O
?
-
-
-
?
valeronitrile + H2O
n-pentanoic acid amide
valeronitrile + H2O
valeramide
additional information
?
-
(1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbonitrile + H2O

(1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbamide
-
substrate conversion: 11.6%, enantiomeric excess: 83.8, 88.9 (methanol), 81.0 (n-hexane)
-
-
r
(1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbonitrile + H2O
(1R,3R)-2,2-dibromo-3-phenylcyclopropanecarbamide
-
substrate conversion: 11.6%, enantiomeric excess: 83.8, 88.9 (methanol), 81.0 (n-hexane)
-
-
r
(1R,3R)-3-phenylcyclopropanecarbonitrile + H2O

(1R,3R)-3-phenylcyclopropanecarbamide
-
substrate conversion: 49.1%, enantiomeric excess: 22.7, 31.1 (methanol), 21.6 (n-hexane)
-
-
r
(1R,3R)-3-phenylcyclopropanecarbonitrile + H2O
(1R,3R)-3-phenylcyclopropanecarbamide
-
substrate conversion: 49.1%, enantiomeric excess: 22.7, 31.1 (methanol), 21.6 (n-hexane)
-
-
r
(1R,3S)-3-phenylcyclopropanecarbonitrile + H2O

(1R,3S)-3-phenylcyclopropanecarbamide
-
substrate conversion: 25.8%, enantiomeric excess: 95.4, 95.4 (methanol), 95.5 (n-hexane)
-
-
r
(1R,3S)-3-phenylcyclopropanecarbonitrile + H2O
(1R,3S)-3-phenylcyclopropanecarbamide
-
substrate conversion: 25.8%, enantiomeric excess: 95.4, 95.4 (methanol), 95.5 (n-hexane)
-
-
r
(1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbonitrile + H2O

(1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbamide
-
substrate conversion: 40.3%, enantiomeric excess: 84.7
-
-
r
(1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbonitrile + H2O
(1S,3S)-2,2-dimethyl-3-phenylcyclopropanecarbamide
-
substrate conversion: 7.9%, enantiomeric excess: 3.2, 5.9 (methanol), 0.7 (n-hexane)
-
-
r
(R)-mandelonitrile + H2O

(R)-mandelamide
wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6%
-
-
?
(R)-mandelonitrile + H2O
(R)-mandelamide
wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6%
-
-
?
(S)-mandelonitrile + H2O

(S)-mandelamide
wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6%
-
-
?
(S)-mandelonitrile + H2O
(S)-mandelamide
wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6%
-
-
?
1,1,3,3,-tetramethylbutylisonitrile + H2O

1,1,3,3,-tetramethylisobutylamide
-
-
-
-
?
1,1,3,3,-tetramethylbutylisonitrile + H2O
1,1,3,3,-tetramethylisobutylamide
-
-
-
-
?
1-cyanocyclohexaneacetonitrile + H2O

1-cyanocyclohexaneacetamide
-
-
-
?
1-cyanocyclohexaneacetonitrile + H2O
1-cyanocyclohexaneacetamide
-
-
-
?
1-naphthylnitrile + H2O

1-naphthylamide
-
-
-
-
?
1-naphthylnitrile + H2O
1-naphthylamide
-
-
-
-
?
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O

2(R)-(4-chloro-phenyl)-3-methyl-butyramide
-
enantioselective hydration
-
?
2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(R)-(4-chloro-phenyl)-3-methyl-butyramide
-
enantioselective hydration
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O

2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
-
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
-
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
enantioselective hydration
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
-
-
?
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
enantioselective hydration
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O

2,2-dimethylcyclopropanecarbamide
-
-
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O
2,2-dimethylcyclopropanecarbamide
-
-
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O

2,2-dimethylcyclopropanecarboxamide
Comamonas oleophilus
-
-
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O
2,2-dimethylcyclopropanecarboxamide
-
-
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O
2,2-dimethylcyclopropanecarboxamide
-
-
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O
2,2-dimethylcyclopropanecarboxamide
-
-
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O

?
29% of the activity compared to 3-cyanopyridine
-
-
?
2,2-dimethylcyclopropanecarbonitrile + H2O
?
-
-
-
?
2,6-dichlorobenzamide + H2O

2,6-dichlorobenzoic acid
-
-
-
-
?
2,6-dichlorobenzamide + H2O
2,6-dichlorobenzoic acid
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O

2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-amino-2,3-dimethylbutyronitrile + H2O
2-amino-2,3-dimethylbutyramide
-
-
-
-
?
2-cyanobenzamide + H2O

benzene-1,2-dicarboxamide
-
-
-
?
2-cyanobenzamide + H2O
benzene-1,2-dicarboxamide
-
-
-
?
2-cyanopyridine + H2O

pyridine-2-carbamide
-
-
-
-
?
2-cyanopyridine + H2O
pyridine-2-carbamide
-
-
-
-
?
2-cyanopyridine + H2O
pyridine-2-carbamide
46% of the activity compared to 3-cyanopyridine
-
-
?
2-cyanopyridine + H2O
pyridine-2-carbamide
46% of the activity compared to 3-cyanopyridine
-
-
?
2-cyanopyridine + H2O
pyridine-2-carbamide
-
-
-
-
?
2-cyanopyridine + H2O
pyridine-2-carbamide
-
-
-
-
?
2-cyanopyridine + H2O
pyridine-2-carbamide
-
-
-
-
?
2-furonitrile + H2O

2-furoamide
-
-
-
-
?
2-furonitrile + H2O
2-furoamide
-
-
-
-
?
2-hydroxypropionitrile + H2O

2-hydroxypropionic acid amide
-
i.e. DL-lactonitrile
-
-
?
2-hydroxypropionitrile + H2O
2-hydroxypropionic acid amide
-
116% of the activity with propionitrile
-
-
?
2-methyl-3-butenenitrile + H2O

?
-
-
-
-
?
2-methyl-3-butenenitrile + H2O
?
-
-
-
-
?
2-naphthylacetonitrile + H2O

2-naphthylacetamide
-
-
-
-
?
2-naphthylacetonitrile + H2O
2-naphthylacetamide
-
-
-
-
?
2-phenylpropionitrile + H2O

2-phenylpropionamide
-
used as well as phenylacetonitrile
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
-
the enzyme is not enantioselective with the compound
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
-
the enzyme is not enantioselective with the compound
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
-
-
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
Rhodococcus sp. Novo SP361
-
-
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
-
the enzyme is highly enantioselective with the compound
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
-
the enzyme is highly enantioselective with the compound
-
-
?
2-phenylpropionitrile + H2O
2-phenylpropionamide
-
the enzyme is highly enantioselective with the compound
-
-
?
3,4,5-trimethoxybenzonitrile + H2O

3,4,5-trimethoxybenzamide
-
conversion rate: 21.71%
-
-
?
3,4,5-trimethoxybenzonitrile + H2O
3,4,5-trimethoxybenzamide
-
conversion rate: 21.71%
-
-
?
3,4-dimethoxybenzonitrile + H2O

3,4-dimethoxybenzaldehyde
-
-
-
-
?
3,4-dimethoxybenzonitrile + H2O
3,4-dimethoxybenzaldehyde
Rhodococcus sp. Novo SP361
-
-
-
-
?
3,4-dimethoxybenzonitrile + H2O

3,4-dimethoxybenzaldehyde + HCN
-
-
-
-
?
3,4-dimethoxybenzonitrile + H2O
3,4-dimethoxybenzaldehyde + HCN
Rhodococcus sp. Novo SP361
-
-
-
-
?
3-(1-cyanoethyl)benzoic acid + H2O

?
-
the enzyme is enantioselective with the compound
-
-
?
3-(1-cyanoethyl)benzoic acid + H2O
?
-
the enzyme is enantioselective with the compound
-
-
?
3-(1-cyanoethyl)benzoic acid + H2O
?
-
the enzyme is enantioselective with the compound
-
-
?
3-benzoyloxyglutaronitrile + H2O

(S)-3-benzoyloxy-4-cyanobutyramide
-
enantiomeric excess: 95%, 5 h
-
-
?
3-benzoyloxyglutaronitrile + H2O
(S)-3-benzoyloxy-4-cyanobutyramide
-
enantiomeric excess: 95%, 5 h
-
-
?
3-benzyloxyglutaronitrile + H2O

3-benzyloxy-4-cyanobutyramide
-
enantiomeric excess: 69%, 30 min
-
-
?
3-benzyloxyglutaronitrile + H2O
3-benzyloxy-4-cyanobutyramide
-
enantiomeric excess: 69%, 30 min
-
-
?
3-chlorobenzonitrile + H2O

3-chlorobenzamide
-
-
-
-
?
3-chlorobenzonitrile + H2O
3-chlorobenzamide
-
95% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-cyanopyridine + H2O

nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid
-
-
?
3-cyanopyridine + H2O
nicotinamide
substrate of recombinant wild-type enzyme, not of mutant enzymes
-
-
?
3-cyanopyridine + H2O
nicotinamide
substrate of recombinant wild-type enzyme, not of mutant enzymes
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
a step in the biosynthesis of nicotinamide, one of the important forms of vitamin B3
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
a step in the biosynthesis of nicotinamide, one of the important forms of vitamin B3
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
H-NHase activity
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
H-NHase activity
-
-
?
3-cyanopyridine + H2O
nicotinamide
-
-
-
-
?
3-cyanopyridine + H2O

pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
lower activity
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
i.e. nicotinamide
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
hydration at 5528% compared to the activity with thiacloprid
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
hydration at 5528% compared to the activity with thiacloprid
-
-
?
3-cyanopyridine + H2O
pyridine-3-carbamide
a niacin precursor
-
-
?
3-cyanopyridine + H2O

pyridine-3-carboxamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carboxamide
-
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carboxamide
-
-
-
?
3-cyanopyridine + H2O
pyridine-3-carboxamide
-
-
-
?
3-hydroxybenzonitrile + H2O

3-hydroxybenzamide
-
-
-
-
?
3-hydroxybenzonitrile + H2O
3-hydroxybenzamide
-
-
-
-
?
3-hydroxybutryronitrile + H2O

3-hydroxybutyramide
-
yield 99%
-
?
3-hydroxybutryronitrile + H2O
3-hydroxybutyramide
-
yield 99%
-
?
3-hydroxypropionitrile + H2O

3-hydroxypropionamide
-
yield 100%
-
?
3-hydroxypropionitrile + H2O
3-hydroxypropionamide
-
yield 100%
-
?
3-hydroxyvaleronitrile + H2O

3-hydroxyvaleramide
-
yield 99%
-
?
3-hydroxyvaleronitrile + H2O
3-hydroxyvaleramide
-
yield 99%
-
?
3-methylbenzonitrile + H2O

3-methylbenzamide
lower activity
-
-
?
3-methylbenzonitrile + H2O
3-methylbenzamide
-
17% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-phenylpropanenitrile + H2O

3-phenylpropanamide
-
-
-
-
ir
3-phenylpropanenitrile + H2O
3-phenylpropanamide
-
99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
3-tolunitrile + H2O

?
-
-
-
-
?
3-tolunitrile + H2O
?
-
-
-
-
?
4-(trifluoromethyl)benzonitrile + H2O

4-(trifluoromethyl)benzamide
-
-
-
-
ir
4-(trifluoromethyl)benzonitrile + H2O
4-(trifluoromethyl)benzamide
-
above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
4-acetylbenzonitrile + H2O

4-acetylbenzamide
-
-
-
-
ir
4-acetylbenzonitrile + H2O
4-acetylbenzamide
-
above 99% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
4-aminobenzonitrile + H2O

4-aminobenzamide
-
-
-
-
?
4-aminobenzonitrile + H2O
4-aminobenzamide
-
-
-
-
?
4-chlorobenzonitrile + H2O

4-chlorobenzamide
-
-
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzamide
-
-
-
-
?
4-chlorobenzonitrile + H2O
4-chlorobenzamide
-
above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
4-cyanobenzamide + H2O

benzene-1,4-dicarboxamide
-
-
-
?
4-cyanobenzamide + H2O
benzene-1,4-dicarboxamide
-
-
-
?
4-cyanopyridine + H2O

isonicotinamide
substrate of recombinant wild-type enzyme, not of mutant enzymes
-
-
?
4-cyanopyridine + H2O
isonicotinamide
substrate of recombinant wild-type enzyme, not of mutant enzymes
-
-
?
4-cyanopyridine + H2O

pyridine-4-carbamide
-
-
-
-
?
4-cyanopyridine + H2O
pyridine-4-carbamide
-
-
-
-
?
4-cyanopyridine + H2O
pyridine-4-carbamide
74% of the activity compared to 3-cyanopyridine
-
-
?
4-cyanopyridine + H2O
pyridine-4-carbamide
74% of the activity compared to 3-cyanopyridine
-
-
?
4-cyanopyridine + H2O
pyridine-4-carbamide
lower activity
-
-
?
4-cyanopyridine + H2O
pyridine-4-carbamide
-
-
-
-
?
4-hydroxybenzonitrile + H2O

4-hydroxybenzoic acid amide
-
-
-
-
?
4-hydroxybenzonitrile + H2O
4-hydroxybenzoic acid amide
-
-
-
-
?
4-hydroxybenzonitrile + H2O
4-hydroxybenzoic acid amide
-
-
-
-
?
4-hydroxyphenylacetonitrile + H2O

4-hydroxyphenylacetamide
-
-
-
-
?
4-hydroxyphenylacetonitrile + H2O
4-hydroxyphenylacetamide
-
-
-
-
?
4-hydroxyphenylacetonitrile + H2O
4-hydroxyphenylacetamide
-
-
-
-
?
4-methoxybenzonitrile + H2O

4-methoxybenzamide
-
-
-
-
ir
4-methoxybenzonitrile + H2O
4-methoxybenzamide
-
above 99% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
4-methylbenzonitrile + H2O

4-methylbenzamide
-
-
-
-
ir
4-methylbenzonitrile + H2O
4-methylbenzamide
-
above 99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
5-cyanovaleramide

adiponitrile + H2O
-
-
-
r
5-cyanovaleramide
adiponitrile + H2O
-
-
-
r
5-hydroxymethyl-2-furonitrile + H2O

5-hydroxymethyl-2-furamide
-
-
-
-
?
5-hydroxymethyl-2-furonitrile + H2O
5-hydroxymethyl-2-furamide
-
-
-
-
?
acetonitrile + H2O

acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
ir
acetonitrile + H2O
acetamide
-
-
-
ir
acetonitrile + H2O
acetamide
-
-
-
-
r
acetonitrile + H2O
acetamide
-
10% of the activity with propionitrile
-
-
?
acetonitrile + H2O
acetamide
-
10% of the activity with propionitrile
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
?
acetonitrile + H2O
acetamide
-
very low activity
-
-
?
acetonitrile + H2O
acetamide
-
-
-
?
acetonitrile + H2O
acetamide
-
very low activity
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
r
acetonitrile + H2O
acetamide
-
-
-
-
r
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
best substrate
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
best substrate
-
-
?
acetonitrile + H2O
acetamide
-
-
-
-
?
acetonitrile + H2O
acetamide
-
79% of the activity with acrylonitrile
-
-
?
acetonitrile + H2O
acetamide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
acetonitrile + H2O
acetamide
-
79% of the activity with acrylonitrile
-
-
?
acetonitrile + H2O
acetamide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
acetonitrile + H2O
acetamide
hydration at 29.02% compared to the activity with thiacloprid
-
-
?
acetonitrile + H2O
acetamide
hydration at 29.02% compared to the activity with thiacloprid
-
-
?
acrylamide + H2O

acrylonitrile
-
-
-
-
?
acrylamide + H2O
acrylonitrile
-
-
-
-
?
acrylamide + H2O
acrylonitrile
-
-
-
-
?
acrylamide + H2O
acrylonitrile
-
-
-
?
acrylamide + H2O
acrylonitrile
-
-
-
?
acrylamide + H2O
acrylonitrile
-
-
-
?
acrylonitrile + H2O

2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
r
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
78% of the activity with propionitrile
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylamide
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylamide
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
-
-
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O
2-propenoic acid amide
-
-
i.e. acrylic acid amide
?
acrylonitrile + H2O

?
-
-
-
?
acrylonitrile + H2O
?
-
-
-
?
acrylonitrile + H2O
?
-
-
-
?
acrylonitrile + H2O
?
-
-
-
?
acrylonitrile + H2O

acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
yield 100%
-
?
acrylonitrile + H2O
acrylamide
-
yield 100%
-
?
acrylonitrile + H2O
acrylamide
79% of the activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + H2O
acrylamide
79% of the activity compared to 3-cyanopyridine
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
best substrate
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
best substrate
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
stereoselective reaction
-
-
?
acrylonitrile + H2O
acrylamide
analysis of the structure model of the enzyme-substrate complex and catalytic mechanism, overview
-
-
?
acrylonitrile + H2O
acrylamide
substrate of recombinant wild-type and mutant enzymes
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
stereoselective reaction
-
-
?
acrylonitrile + H2O
acrylamide
analysis of the structure model of the enzyme-substrate complex and catalytic mechanism, overview
-
-
?
acrylonitrile + H2O
acrylamide
substrate of recombinant wild-type and mutant enzymes
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
highest activity
-
-
?
acrylonitrile + H2O
acrylamide
-
highest activity
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
-
-
-
r
acrylonitrile + H2O
acrylamide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
acrylonitrile + H2O
acrylamide
-
-
-
-
r
acrylonitrile + H2O
acrylamide
-
-
-
-
?
acrylonitrile + H2O
acrylamide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
acrylonitrile + H2O
acrylamide
-
-
-
-
?
adiponitrile + H2O

5-cyanovaleramide
-
-
-
-
?
adiponitrile + H2O
5-cyanovaleramide
-
-
-
-
?
adiponitrile + H2O
5-cyanovaleramide
wild-type enzyme forms only adipamide after a 4 h reaction. Y68T and W72Y mutations cause a significant shift in product formation and form primarily 5-cyanovaleramide. Mutant enzyme Y68T/W72Y produces 100% 5-cyanovaleramide
-
-
r
adiponitrile + H2O
5-cyanovaleramide
wild-type enzyme forms only adipamide after a 4 h reaction. Y68T and W72Y mutations cause a significant shift in product formation and form primarily 5-cyanovaleramide. Mutant enzyme Y68T/W72Y produces 100% 5-cyanovaleramide
-
-
r
adiponitrile + H2O

adipamide
-
yield 100%
-
?
adiponitrile + H2O
adipamide
-
yield 100%
-
?
adiponitrile + H2O

adipic acid amide
-
-
-
-
?
adiponitrile + H2O
adipic acid amide
-
108% of the activity with propionitrile
-
-
?
adiponitrile + H2O
adipic acid amide
-
-
-
-
?
adiponitrile + H2O
adipic acid amide
wild-type enzyme forms only adipamide after a 4 h reaction. Y68T and W72Y mutations cause a significant shift in product formation and form primarily 5-cyanovaleramide. Mutant enzyme Y68T/W72Y produces 100% 5-cyanovaleramide
-
-
?
adiponitrile + H2O
adipic acid amide
wild-type enzyme forms only adipamide after a 4 h reaction. Y68T and W72Y mutations cause a significant shift in product formation and form primarily 5-cyanovaleramide. Mutant enzyme Y68T/W72Y produces 100% 5-cyanovaleramide
-
-
?
adiponitrile + H2O
adipic acid amide
-
-
-
-
?
adiponitrile + H2O
adipic acid amide
-
-
-
-
?
alpha-methylbenzyl cyanide + H2O

2-phenylpropanamide
65% of the activity compared to 3-cyanopyridine
-
-
?
alpha-methylbenzyl cyanide + H2O
2-phenylpropanamide
-
-
-
-
r
an aliphatic amide

a nitrile + H2O
-
-
-
-
?
an aliphatic amide
a nitrile + H2O
-
ligand exchange reactions, overview
-
-
?
an aliphatic amide
a nitrile + H2O
-
-
-
-
?
an aliphatic amide
a nitrile + H2O
-
ligand exchange reactions, overview
-
-
?
an aliphatic amide
a nitrile + H2O
-
-
-
-
?
an aliphatic amide
a nitrile + H2O
-
ligand exchange reactions, overview
-
-
?
an aliphatic amide
a nitrile + H2O
-
-
-
-
?
an aliphatic amide
a nitrile + H2O
-
ligand exchange reactions, overview
-
-
?
benzonitrile + H2O

?
-
-
-
?
benzonitrile + H2O
?
-
-
-
?
benzonitrile + H2O

benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4
-
-
?
benzonitrile + H2O
benzamide
82% of the activity compared to 3-cyanopyridine
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4
-
-
?
benzonitrile + H2O
benzamide
-
-
-
?
benzonitrile + H2O
benzamide
lower activity
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
substrate of recombinant wild-type and mutant enzymes
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
substrate of recombinant wild-type and mutant enzymes
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?, ir
benzonitrile + H2O
benzamide
-
99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
benzonitrile + H2O
benzamide
-
-
-
-
?, ir
benzonitrile + H2O
benzamide
-
99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
benzonitrile + H2O
benzamide
hydration at 2532% compared to the activity with thiacloprid
-
-
?
benzonitrile + H2O
benzamide
hydration at 2532% compared to the activity with thiacloprid
-
-
?
benzonitrile + H2O

benzoic acid amide
-
1.3% of the activity with propionitrile
-
-
?
benzonitrile + H2O
benzoic acid amide
-
-
-
-
?
benzonitrile + H2O
benzoic acid amide
-
-
-
-
?
benzonitrile + H2O
benzoic acid amide
-
15% of the activity with acrylonitrile
-
-
?
benzonitrile + H2O
benzoic acid amide
-
low activity
-
-
?
benzonitrile + H2O
benzoic acid amide
-
low activity
-
-
?
benzonitrile + H2O
benzoic acid amide
-
-
-
-
?
benzonitrile + H2O
benzoic acid amide
-
-
-
-
?
benzonitrile + H2O
benzoic acid amide
-
-
-
-
?
benzonitrile + H2O
benzoic acid amide
-
-
-
-
?
benzonitrile + H2O
benzoic acid amide
-
19.4% of the activity with acrylonitrile
-
-
?
benzonitrile + hydroxylamine + H2O

benzohydroxamic acid + NH3
-
-
-
-
?
benzonitrile + hydroxylamine + H2O
benzohydroxamic acid + NH3
-
performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4
-
-
?
benzonitrile + hydroxylamine + H2O
benzohydroxamic acid + NH3
-
-
-
-
?
benzonitrile + hydroxylamine + H2O
benzohydroxamic acid + NH3
-
performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4
-
-
?
bromoxynil + H2O

?
-
-
-
-
?
bromoxynil + H2O
?
-
-
-
-
?
bromoxynil + H2O
?
-
-
-
-
?
bromoxynil + H2O
?
-
-
-
-
?
butyronitrile + H2O

butyramide
-
yield 100%
-
?
butyronitrile + H2O
butyramide
-
-
-
-
?
butyronitrile + H2O
butyramide
248% of the activity compared to 3-cyanopyridine
-
-
?
butyronitrile + H2O
butyramide
-
-
-
-
?
butyronitrile + H2O
butyramide
-
-
-
-
?
butyronitrile + H2O
butyramide
-
-
-
-
?
butyronitrile + H2O
butyramide
-
-
-
-
?
butyronitrile + H2O
butyramide
-
-
-
-
?
butyronitrile + H2O
butyramide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
butyronitrile + H2O
butyramide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
chloroacetonitrile + H2O

chloroacetamide
-
-
-
ir
chloroacetonitrile + H2O
chloroacetamide
-
-
-
ir
chloroacetonitrile + H2O
chloroacetamide
-
42% of the activity with propionitrile
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
42% of the activity with propionitrile
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
-
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
-
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
-
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
-
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
-
-
-
?
chloroacetonitrile + H2O
chloroacetamide
-
-
-
-
?
chloroxynil acid + H2O

?
-
-
-
-
?
chloroxynil acid + H2O
?
-
-
-
-
?
chloroxynil acid + H2O
?
-
-
-
-
?
chloroxynil acid + H2O
?
-
-
-
-
?
chloroxynil amide + H2O

?
-
-
-
-
?
chloroxynil amide + H2O
?
-
-
-
-
?
crotononitrile + H2O

(E)-2-butenoic acid amide
-
19% of the activity with propionitrile
-
-
?
crotononitrile + H2O
(E)-2-butenoic acid amide
-
19% of the activity with propionitrile
-
-
?
crotononitrile + H2O
(E)-2-butenoic acid amide
-
-
-
-
?
crotononitrile + H2O
(E)-2-butenoic acid amide
-
19% of the activity with propionitrile
-
-
?
crotononitrile + H2O
(E)-2-butenoic acid amide
-
-
-
-
?
cyanide + H2O

formamide
-
2% of the activity with propionitrile
-
-
?
cyanide + H2O
formamide
-
-
-
-
?
cyanovaleramide

valerodinitrile + H2O
-
-
-
-
?
cyanovaleramide
valerodinitrile + H2O
-
-
-
-
?
cyanovaleric acid + H2O

?
-
-
-
-
?
cyanovaleric acid + H2O
?
-
-
-
-
?
cyanovaleric acid + H2O
?
-
-
-
-
?
dichlobenil amide + H2O

?
-
-
-
-
?
dichlobenil amide + H2O
?
-
-
-
-
?
ethyl 4-cyanobenzoate + H2O

ethyl 4-carbamoylbenzoate
-
-
-
-
ir
ethyl 4-cyanobenzoate + H2O
ethyl 4-carbamoylbenzoate
-
95% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
ethylene cyanhydrine + H2O

?
-
-
-
-
?
ethylene cyanhydrine + H2O
?
-
-
-
-
?
furan-2-carbonitrile + H2O

furan-2-carboxamide
-
-
-
-
ir
furan-2-carbonitrile + H2O
furan-2-carboxamide
-
99.9% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
hydroxyacetonitrile + H2O

hydroxyacetamide
-
-
-
?
hydroxyacetonitrile + H2O
hydroxyacetamide
-
-
-
r
hydroxyacetonitrile + H2O
hydroxyacetamide
-
50% of the activity with propionitrile
-
?
indole-3-acetonitrile + H2O

(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indol-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O

(indole-3-yl)acetamide
-
-
-
?
indole-3-acetonitrile + H2O
(indole-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indole-3-yl)acetamide
-
-
-
-
?
indole-3-acetonitrile + H2O
(indole-3-yl)acetamide
hydration at 479.79% compared to the activity with thiacloprid
-
-
?
indole-3-acetonitrile + H2O
(indole-3-yl)acetamide
hydration at 479.79% compared to the activity with thiacloprid
-
-
?
indole-3-acetonitrile + H2O

indole-3-acetamide
-
conversion rate: 34.44%
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetamide
-
conversion rate: 34.44%
-
-
?
indole-3-nitrile + H2O

indole-3-acetamide
-
-
-
?
indole-3-nitrile + H2O
indole-3-acetamide
-
the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid
-
?
ioxynil acid + H2O

?
-
-
-
-
?
ioxynil acid + H2O
?
-
-
-
-
?
isobutyronitrile + H2O

isobutyramide
215% of the activity compared to 3-cyanopyridine
-
-
?
isobutyronitrile + H2O
isobutyramide
-
-
-
?
isobutyronitrile + H2O
isobutyramide
-
-
-
-
?
isobutyronitrile + H2O
isobutyramide
-
-
-
-
?
isobutyronitrile + H2O
isobutyramide
hydration at 3.78% compared to the activity with thiacloprid
-
-
?
isobutyronitrile + H2O

isobutyric acid amide
-
113% of the activity with propionitrile
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
113% of the activity with propionitrile
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
71% of the activity with acrylonitrile
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
71% of the activity with acrylonitrile
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
-
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
62.5% of the activity with acrylonitrile
-
-
?
isobutyronitrile + H2O
isobutyric acid amide
-
62.5% of the activity with acrylonitrile
-
-
?
isovaleronitrile + H2O

isovaleric acid amide
-
more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate
-
-
?
isovaleronitrile + H2O
isovaleric acid amide
-
-
product identification by liquid chromatography tandem mass spectrometry
-
?
isovaleronitrile + H2O
isovaleric acid amide
-
-
product identification by liquid chromatography tandem mass spectrometry
-
?
mandelonitrile + H2O

?
31% of the activity compared to 3-cyanopyridine
-
-
?
mandelonitrile + H2O
?
-
used as well as phenylacetonitrile
-
-
?
mandelonitrile + H2O
?
-
-
-
-
?
methacrylamide + H2O

?
-
causes the greatest induction of activity
-
-
?
methacrylamide + H2O
?
-
causes the greatest induction of activity
-
-
?
methacrylonitrile + H2O

methacrylamide
-
yield 100%
-
?
methacrylonitrile + H2O
methacrylamide
substrate of recombinant wild-type and mutant enzymes
-
-
?
methacrylonitrile + H2O
methacrylamide
substrate of recombinant wild-type and mutant enzymes
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
low activity
-
-
?
methacrylonitrile + H2O
methacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methacrylamide
-
low activity
-
-
?
methacrylonitrile + H2O

methylacrylamide
96% of the activity compared to 3-cyanopyridine
-
-
?
methacrylonitrile + H2O
methylacrylamide
-
-
-
-
?
methacrylonitrile + H2O
methylacrylamide
-
-
-
-
?
methacrylonitrile + H2O

methylacrylic acid amide
-
-
-
r
methacrylonitrile + H2O
methylacrylic acid amide
-
53% of the activity with propionitrile
-
-
?
methacrylonitrile + H2O
methylacrylic acid amide
-
more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate
-
-
?
methacrylonitrile + H2O
methylacrylic acid amide
-
-
-
-
?
methacrylonitrile + H2O
methylacrylic acid amide
-
-
-
-
?
methacrylonitrile + H2O
methylacrylic acid amide
-
-
-
-
?
methacrylonitrile + H2O
methylacrylic acid amide
-
-
-
-
?
methyl 4-cyanobenzoate + H2O

methyl 4-carbamoylbenzoate
-
-
-
-
ir
methyl 4-cyanobenzoate + H2O
methyl 4-carbamoylbenzoate
-
98% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
n-butyronitrile + H2O

n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
r
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
r
n-butyronitrile + H2O
n-butyric acid amide
-
33% of the activity with propionitrile
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
140% of the activity with propionitrile
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
33% of the activity with propionitrile
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
140% of the activity with propionitrile
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-butyronitrile + H2O
n-butyric acid amide
-
-
-
-
?
n-capronitrile + H2O

n-hexanoic acid amide
-
46% of the activity with propionitrile
-
-
?
n-capronitrile + H2O
n-hexanoic acid amide
-
46% of the activity with propionitrile
-
-
?
n-capronitrile + H2O
n-hexanoic acid amide
-
-
-
-
?
n-capronitrile + H2O
n-hexanoic acid amide
-
-
-
-
?
n-valeronitrile + H2O

n-valeramide
-
-
-
-
?
n-valeronitrile + H2O
n-valeramide
-
-
-
-
?
naproxennitrile + H2O

?
-
the enzyme is enantioselective with the compound
-
-
?
naproxennitrile + H2O
?
-
the enzyme is enantioselective with the compound
-
-
?
naproxennitrile + H2O
?
-
the enzyme is enantioselective with the compound
-
-
?
nicotinonitrile + H2O

nicotinamide
-
-
-
-
?
nicotinonitrile + H2O
nicotinamide
-
-
-
-
?
o-chlorobenzonitrile + H2O

o-chlorobenzamide
-
-
-
-
?
o-chlorobenzonitrile + H2O
o-chlorobenzamide
-
-
-
-
?
phenylacetonitrile + H2O

2-phenylacetamide
-
-
-
-
ir
phenylacetonitrile + H2O
2-phenylacetamide
-
28% conversion, in phosphate buffer pH 7.0, at 30°C
-
-
?
phenylacetonitrile + H2O

phenylacetamide
-
337% activity compared to indole-3-acetonitrile
-
-
?
phenylacetonitrile + H2O
phenylacetamide
-
337% activity compared to indole-3-acetonitrile
-
-
?
phenylacetonitrile + H2O
phenylacetamide
-
-
-
-
?
phenylacetonitrile + H2O
phenylacetamide
-
-
-
-
?
phenylacetonitrile + H2O
phenylacetamide
-
337% activity compared to indole-3-acetonitrile
-
-
?
phenylacetonitrile + H2O
phenylacetamide
-
337% activity compared to indole-3-acetonitrile
-
-
?
pivalonitrile + H2O

2,2-dimethylpropionic acid amide
-
5.3% of the activity with propionitrile
-
-
?
pivalonitrile + H2O
2,2-dimethylpropionic acid amide
-
5% of the activity with propionitrile
-
-
?
pivalonitrile + H2O
2,2-dimethylpropionic acid amide
-
-
-
-
?
pivalonitrile + H2O
2,2-dimethylpropionic acid amide
-
62% of the activity with acrylonitrile
-
-
?
pivalonitrile + H2O
2,2-dimethylpropionic acid amide
-
62% of the activity with acrylonitrile
-
-
?
propionitrile + H2O

propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
113% of the activity compared to 3-cyanopyridine
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
?
propionitrile + H2O
propionamide
-
-
-
-
r
propionitrile + H2O
propionamide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
propionitrile + H2O
propionamide
-
-
-
-
r
propionitrile + H2O
propionamide
-
substrate specificity: acetonitrile ~ propionitrile > acrylonitrile >> butyronitrile
-
-
r
propionitrile + H2O

propionic acid amide
-
-
-
-
?
propionitrile + H2O
propionic acid amide
-
-
i.e. propionamide
r
propionitrile + H2O
propionic acid amide
-
-
i.e. propionamide
r
propionitrile + H2O
propionic acid amide
-
-
-
-
?
propionitrile + H2O
propionic acid amide
-
-
-
?
propionitrile + H2O
propionic acid amide
-
-
i.e. propionamide
?
propionitrile + H2O
propionic acid amide
-
-
-
-
?
propionitrile + H2O
propionic acid amide
-
-
i.e. propionamide
<