EC Number   |
Application |
Reference |
|---|
    2.5.1.6 | drug development |
the enzyme form has a less extensive subunit interface than some previously determined structures, and contains some key structural differences from the human enzyme in an allosteric site, presenting an opportunity for the design of selective inhibitors against the S-adenosylmethionine synthetase from this organism |
-, 758605 |
| 2.5.1.6 | drug development |
the product S-adenosyl-L-methionine is an effective cure for liver disease, depressive syndromes, and osteoarthritis. A multiple-copy integrative plasmid pYMIKP-SAM2 is introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001. The recombinant yeast exhibits higher enzymatic activity of methionine adenosyltransferase and improved S-adenosyl-L-methionine biosynthesis. With a three-phase fed-batch strategy in 15-liter bench-top fermentor, 8.81 g/l S-adenosyl-L-methionine is achieved after 52 h cultivation of R1-ZJU001, about 27.1 % increase over its parent strain ZJU001, whereas the S-adenosyl-L-methionine content is also improved from 64.6 mg/g dry cell weight to 91.0 mg/g dry cell weight |
-, 758662 |
| 2.5.1.6 | medicine |
animals fed ethanol intragastrically for 9 weeks switch hepatic MAT expression from MAT1A to MAT2A followed by decrease in S-adenosylmethionine levels, hypomethylation of c-myc, increase in c-myc expression and increased DNA strand break accumulation |
657503 |
| 2.5.1.6 | medicine |
MAT1A knockout mice, spontaneous steatohepatitis develops by 8 months, hepatocellular carcinoma develops by 18 months |
657503 |
| 2.5.1.6 | medicine |
patients with acoholic liver disease, decrease in hepatic enzyme activity due to decreased MAT1A expression and inactivation of MAT1A-encoded isoenzymes |
657503 |
| 2.5.1.6 | medicine |
S-adenosyl-L-methionine is applied to patients with alcoholic liver disease |
675169 |
| 2.5.1.6 | synthesis |
improved production of erythromycin A by expression of a heterologous gene encoding S-adenosylmethionine synthetase in Saccharopolyspora erythraea |
671563 |
| 2.5.1.6 | synthesis |
recombination of MAT genes from Escherichia coli, Saccharomyces cerevisiae, and Streptomyces spectabilis by DNA shuffling and transformation into Pichia pastoris. In the two best recombinant strains, the MAT activities are respectively 201% and 65% higher than the recombinant strains containing the starting MAT genes, and the SAM concentration increases by 103% and 65%, respectively. A 6.14 g/l of SAM production is reached in a 500 l bioreactor with the best recombinant strain |
704764 |
| 2.5.1.6 | synthesis |
recombination of MAT genes from Escherichia coli, Saccharomyces cerevisiae, and Streptomyces spectabilis by DNA shuffling and transformation into Pichia pastoris. In the two best recombinant strains, the MAT activities are respectively 201% and 65% higher than the recombinant strains containing the starting MAT genes, and the SAM concentration increases by 103% and 65%, respectively. The K18R mutation of Streptomyces spectabilis probably result in the increased activity of the best MAT. A 6.14 g/l of SAM production is reached in a 500 l bioreactor with the best recombinant strain |
704764 |
