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Information on EC 2.5.1.6 - methionine adenosyltransferase
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EC Tree
2.5.1.6 methionine adenosyltransferaseSpecify your search results
Word Map
- 2.5.1.6
-
self-assembled
- gold
-
adsorption
-
film
- alkanethiols
-
photoelectron
-
adsorb
-
electrochemical
-
infrared
-
fabric
-
electrode
-
ellipsometry
-
devices
-
voltammetry
-
coverage
-
tunnel
-
thiolate
-
silicon
- s-adenosylhomocysteine
-
interfacial
-
impedance
-
single-component
-
nanoscale
- ferrocene
-
microbalance
-
wafer
-
field-effect
-
polycrystalline
-
transistor
-
transsulfuration
-
photovoltaic
-
wettability
-
electroactive
-
statin-associated
- medicine
- tripolyphosphate
-
silane
-
lithography
- synthesis
-
wettabl
-
electrochemistry
- quartz
-
electrodeposition
- azobenzene
-
surface-enhanced
-
micropatterned
-
semiconductor
-
transmethylation
-
thin-film
-
headgroups
-
well-ordered
-
stamp
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
adenosylmethionine synthetase, AdoMet synthase, AdoMet synthease, AdoMet synthetase, AdoMetS, ATP-methionine adenosyltransferase, ATP: L-methionine S-adenosyltransferase, EC 2.4.2.13, MAT, MAT I, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosylmethionine synthetase
-
-
-
-
AdoMet synthease
AdoMet synthetase
AdoMetS
ATP-methionine adenosyltransferase
-
-
-
-
EC 2.4.2.13
-
-
formerly
-
MAT
MAT I
MAT II
MAT III
MAT2
MAT2A
methionine adenosyltransferase
methionine adenosyltransferase 2A
methionine S-adenosyltransferase
-
-
-
-
methionine-activating enzyme
-
-
-
-
MetK
Mj-MAT
MJ1208
S-adenosyl-L-methionine synthetase
S-adenosyl-Lmethionine synthetase
S-adenosylmethionine synthase
S-adenosylmethionine synthetase
SAM synthase
SAM synthetase
SAM-s
SAMS
SAMS1
SSO0199
AdoMet synthetase
-
-
-
-
methionine adenosyltransferase
-
-
-
-
S-adenosyl-L-methionine synthetase
-
-
-
-
S-adenosylmethionine synthase
-
-
-
-
S-adenosylmethionine synthetase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
mechanism
-
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
steady state ordered Bi Ter mechanism with ATP adding before L-methionine and S-adenosylmethionine being the first product released, random release of phosphate and diphosphate
-
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
neutral H14 acts as an acid to cleave the C5’-O5’ bond of ATP, S of methionine makes a nucleophilic attack on the C5’ to form the product
-
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
mechanism of inhibiton by product analogues and implications for reaction
-
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
mechanism, key role of K182 in the correct positioning of the substrates, and D135 in stabilizing the sulfonium group formed in the product
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
catalysis of a SN2 reaction through hydrogen bonding of the liberated oxygen-5' to the histidine, charge neutralization by the two Mg2+ ions, and stabilization of the product sulfonium cation through a close, non-bonded, contact between the sulfur and the ribose oxygen-4'
-
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
adenosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
ATP:L-methionine S-adenosyltransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
9012-52-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
ATP + (2S)-2-amino-4-(butylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(butylsulfanyl)butanoic acid
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(ethylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(ethylselanyl)butanoic acid
76% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(ethylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(ethylsulfanyl)butanoic acid
84% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid
30% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid
28% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid
36% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid
40% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid
53% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propylselanyl)butanoic acid
66% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propylsulfanyl)butanoic acid
44% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid
50% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methyl propyl)sulfanyl] butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methyl propyl)sulfanyl]butanoic acid
57% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid
46% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid
15% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid
25% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid
60% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methyl butyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methyl butyl)sulfanyl] butanoic acid
36% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid
44% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid
12% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(cyanomethyl) sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(cyanomethyl)sulfanyl]butanoic acid
68% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid
12% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid
38% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid
24% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid
28% of the turnover rate compared to L-methionine
-
-
?
ATP + D-methionine + H2O
S-adenosyl-D-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-ethionine + H2O
phosphate + diphosphate + S-adenosyl-L-ethionine
-
-
-
?
ATP + L-methionine methyl ester + H2O
S-adenosyl-L-methionine methyl ester + phosphate + diphosphate
-
-
-
?
ATP + L-selenomethionine + H2O
phosphate + diphosphate + Se-adenosyl-L-selenomethionine
as active as L-methionine
-
-
?
CTP + L-methionine + H2O
S-cytosyl-L-methionine + phosphate + diphosphate
-
-
-
?
GTP + L-methionine + H2O
S-guanosyl-L-methionine + phosphate + diphosphate
-
-
-
?
UTP + L-methionine + H2O
S-urasyl-L-methionine + phosphate + diphosphate
-
-
-
?
2'-deoxy-ATP + L-methionine + H2O
?
-
-
-
-
?
2'-deoxy-ATP + L-methionine + H2O
?
-
completely specific for ATP
-
-
?
3'-deoxy-ATP + L-methionine + H2O
?
-
-
-
-
?
3'-deoxy-ATP + L-methionine + H2O
?
-
completely specific for ATP
-
-
?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
10% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
10% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
30% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
30% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
70% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
70% of the turnover rate compared to L-methionine
-
-
?
ATP + L-ethionine + H2O
S-adenosyl-L-ethionine + phosphate + diphosphate
-
-
-
-
ATP + L-ethionine + H2O
S-adenosyl-L-ethionine + phosphate + diphosphate
-
-
-
?
ATP + L-ethionine + H2O
S-adenosyl-L-ethionine + phosphate + diphosphate
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
aspartate family biosynthesis pathway and methionine metabolism, regulation, S-adenosyl-L-methionine activates threonine synthase expression and negatively regulates the transcript level of the cystathionine gamma-synthase, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
S-adenosyl-L-methionine is required for betaine synthesis and also for the synthesis of other compounds, especially lignin, transcript levels of the enzyme are co-regulated with those of phosphoethanolamine N-methyltransferase and choline monooxygenase to supply S-adenosyl-L-methionine for betaine synthesis in the leaves, overview, enzyme regulation pattern in plant tissues, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
biosynthesis of the key compound in the trans-methylation reactions
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
the S(MK) box, a conserved RNA motif in the 5'-untranslated region of the metK gene, is a SAM-binding RNA responsible for translational regulation of the enzyme, it binds specifically to S-adenosyl-L-methionine in vitro and in vivo causing a structural RNA rearrangement that causes a sequestration of the Shine-Dalgarno sequence, structural mapping and mechanism, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
an essential enzyme that catalyzes the formation of the principal methyl donor S-adenosylmethionine, S-adenosyl-L-methionine is also a key metabolite that regulates hepatocyte growth, death and differentiation, molecular mechanism, overview, abnormalities in MAT and decreased S-adenosyl-L-methionine levels occur in humans with alcoholic liver disease, chronic hepatic S-adenosyl-L-methionine deficiency can result in the spontaneous development of steatohepatitis and hepatocellular carcinoma, overview, hepatic S-adenosyl-L-methionine biosynthesis and methionine metabolism, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
rate-limiting enzyme of the S-adenosyl-L-methionine synthesis pathway, cell methionine and S-adenosylmethionine contents increase in response to hyperoxia in SAE and A549 cells, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
a post-translational mechanism is involved in MAT regulation, the enzyme is down-regulated in pathological processes such as liver cirrhosis, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
biosynthesis of S-adenosyl-L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
two-step reaction in which reaction of ATP and methionine initially yields the S-adenosyl-L-methionine and tripolyphosphate. The tripolyphosphate remains enzyme-bound and is cleaved to diphosphate and phosphate before product release
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
biosynthesis of S-adenosyl-L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
two-step reaction in which reaction of ATP and methionine initially yields the S-adenosyl-L-methionine and tripolyphosphate. The tripolyphosphate remains enzyme-bound and is cleaved to diphosphate and phosphate before product release
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q3TH56
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
regulation of methionine metabolism, S-adenosyl-L-methionine metabolic pathway, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
aspartate family biosynthesis pathway and methionine metabolism,regulation, S-adenosyl-L.methionine activates threonine synthase expression and negatively regulates the transcript level of the cystathionine gamma-synthase, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
typhus group rickettsiae have the capability of synthesizing as well as transporting SAM
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q3HW35
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
ir
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
methyl donor in transmethylation reactions and as propylamine donor for polyamine biosynthesis
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
mechanism
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
the product S-adenosylmethionine is important as a direct metabolic donor of methyl and alpha-amino-n-butyryl groups
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
two reaction steps: S-adenosylmethionine synthesis and tripolyphosphate hydrolysis. Tripolyphosphate hydrolysis is the rate determining reaction
-
-
-
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
MAT activity controls cellular glutathione levels, polyamine synthesis and folate cycling
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
completely specific for ATP
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
used as aminopropyl group donor in synthesis of polyamines and is also the methyl group donor for most cellular methyltransferase reactions
-
?
ATP + methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
two isoenzymes with different behavior on exogenous S-adenosylmethionine addition
-
?
additional information
?
-
-
protein S-nitrosylation by NO represents a redox-based regulation mechanism playing a pivotal role in plants, MAT catalyzes the synthesis of the ethylene precursor S-adenosylmethionine and NO influences ethylene production in plants, the enzyme probably mediates the cross-talk between ethylene and NO signaling, overview
-
-
-
additional information
?
-
-
genes in the S-box family are regulated by binding of S-adenosylmethionine to the 5' region of the mRNA of the regulated gene, SAM binding promotes a rearrangement of the RNA structure that results in premature termination of transcription in vitro and repression of expression of the downstream coding sequence, the S-box RNA element therefore acts as a SAM-binding riboswitch in vitro
-
-
-
additional information
?
-
-
N-acetyl-L-methionine and N,N-dimethyl-L-methionine are very poor substrates
-
-
-
additional information
?
-
-
ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate
-
-
-
additional information
?
-
-
ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate
-
-
-
additional information
?
-
S-adenosylmethionine synthesis and tripolyphosphatase activity messured for six aspartate-mutants
-
-
-
additional information
?
-
transmethylation and transsulfuration pathways, overview
-
-
-
additional information
?
-
-
no activity with methional, methioninol, 3-methylthiopropylamine
-
-
-
additional information
?
-
-
N-acetyl-L-methionine and N,N-dimethyl-L-methionine are very poor substrates
-
-
-
additional information
?
-
-
S-adenosylmethionine synthetase A exhibits tripolyphosphatase activity
-
-
-
additional information
?
-
-
S-adenosylmethionine synthetase B exhibits tripolyphosphatase activity
-
-
-
additional information
?
-
the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP
-
-
-
additional information
?
-
the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP
-
-
-
additional information
?
-
-
ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate
-
-
-
additional information
?
-
-
methionine can be substituted by selenomethionine, alpha-methyl-DL-methionine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
aspartate family biosynthesis pathway and methionine metabolism, regulation, S-adenosyl-L-methionine activates threonine synthase expression and negatively regulates the transcript level of the cystathionine gamma-synthase, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q6F3F0, Q6F3F1, Q6F3F3, Q7XZR1
S-adenosyl-L-methionine is required for betaine synthesis and also for the synthesis of other compounds, especially lignin, transcript levels of the enzyme are co-regulated with those of phosphoethanolamine N-methyltransferase and choline monooxygenase to supply S-adenosyl-L-methionine for betaine synthesis in the leaves, overview, enzyme regulation pattern in plant tissues, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q9K5E4
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q9K5E4
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q6J2L0
biosynthesis of the key compound in the trans-methylation reactions
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
the S(MK) box, a conserved RNA motif in the 5'-untranslated region of the metK gene, is a SAM-binding RNA responsible for translational regulation of the enzyme, it binds specifically to S-adenosyl-L-methionine in vitro and in vivo causing a structural RNA rearrangement that causes a sequestration of the Shine-Dalgarno sequence, structural mapping and mechanism, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q9NZL9
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
P31153, Q00266
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
an essential enzyme that catalyzes the formation of the principal methyl donor S-adenosylmethionine, S-adenosyl-L-methionine is also a key metabolite that regulates hepatocyte growth, death and differentiation, molecular mechanism, overview, abnormalities in MAT and decreased S-adenosyl-L-methionine levels occur in humans with alcoholic liver disease, chronic hepatic S-adenosyl-L-methionine deficiency can result in the spontaneous development of steatohepatitis and hepatocellular carcinoma, overview, hepatic S-adenosyl-L-methionine biosynthesis and methionine metabolism, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
P31153
rate-limiting enzyme of the S-adenosyl-L-methionine synthesis pathway, cell methionine and S-adenosylmethionine contents increase in response to hyperoxia in SAE and A549 cells, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
a post-translational mechanism is involved in MAT regulation, the enzyme is down-regulated in pathological processes such as liver cirrhosis, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
A4ULF8
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
biosynthesis of S-adenosyl-L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
biosynthesis of S-adenosyl-L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
regulation of methionine metabolism, S-adenosyl-L-methionine metabolic pathway, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
aspartate family biosynthesis pathway and methionine metabolism,regulation, S-adenosyl-L.methionine activates threonine synthase expression and negatively regulates the transcript level of the cystathionine gamma-synthase, overview
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q8TZW1
the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
typhus group rickettsiae have the capability of synthesizing as well as transporting SAM
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
P43280
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q3HW35
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Q72I53
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Q72I53
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
ir
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
methyl donor in transmethylation reactions and as propylamine donor for polyamine biosynthesis
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
the product S-adenosylmethionine is important as a direct metabolic donor of methyl and alpha-amino-n-butyryl groups
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
two reaction steps: S-adenosylmethionine synthesis and tripolyphosphate hydrolysis. Tripolyphosphate hydrolysis is the rate determining reaction
-
-
-
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
MAT activity controls cellular glutathione levels, polyamine synthesis and folate cycling
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
used as aminopropyl group donor in synthesis of polyamines and is also the methyl group donor for most cellular methyltransferase reactions
-
?
additional information
?
-
-
protein S-nitrosylation by NO represents a redox-based regulation mechanism playing a pivotal role in plants, MAT catalyzes the synthesis of the ethylene precursor S-adenosylmethionine and NO influences ethylene production in plants, the enzyme probably mediates the cross-talk between ethylene and NO signaling, overview
-
-
-
additional information
?
-
-
genes in the S-box family are regulated by binding of S-adenosylmethionine to the 5' region of the mRNA of the regulated gene, SAM binding promotes a rearrangement of the RNA structure that results in premature termination of transcription in vitro and repression of expression of the downstream coding sequence, the S-box RNA element therefore acts as a SAM-binding riboswitch in vitro
-
-
-
additional information
?
-
P31153
transmethylation and transsulfuration pathways, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
Q3HW35
the binding site contains the conserved nanopeptide 276GGGAFSGKD284 for the P-loop
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
K+
Mg2+
Mn2+
Na+
NH4+
Zn2+
additional information
Co2+
-
for maximal activation the cation concentration must be at least equal to ATP concentration
Co2+
-
can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B; can replace Mg2+ with lower relative activity
K+
monovalent cations required for optimal activity, 50 mM K+ sufficient for full activity, can be replaced by NH4+ but not by Na+; monovalent cations required for optimal activity, 50 mM K+ sufficient for full activity, can be replaced by NH4+ but not by Na+; monovalent cations required for optimal activity, 50 mM K+ sufficient for full activity, can be replaced by NH4+ but not by Na+
Mg2+
strictly dependent on divalent cations with maximum activity at 5mM and Mg2+ fully replaceable by Mn2+ or Co2+ salts; strictly dependent on divalent cations with maximum activity at 5mM and Mg2+ fully replaceable by Mn2+ or Co2+ salts; strictly dependent on divalent cations with maximum activity at 5mM and Mg2+ fully replaceable by Mn2+ or Co2+ salts
Mg2+
-
cation concentration must be at least equal to ATP concentration for maximal activity
Mg2+
-
Mg2+ in excess of that bound to ATP and EDTA is required for optimal activity
Mg2+
-
no activity is detectable in the absence of Mg2+, and maximal activity is observed at 10 mM MgCl2 when ATP is present at 5 mM, indicating that MgATP is probably the true substrate
Mg2+
absolutely dependent upon the presence of Mg2+, suggesting that a Mg2+-ATP complex functions as substrate. Maximal activity is obtained at a Mg2+ concentration of 20 mM
Mg2+
-
maximal activation at 40 mM; S-adenosylmethionine synthetase A is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM); S-adenosylmethionine synthetase B is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM)
Mg2+
Q3HW35
the enzyme requires Mn2+ or Mg2+, which are equally active, bound by D17 and D289
Mn2+
-
for maximal activation the cation concentration must be at least equal to ATP concentration
Mn2+
-
can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase A; can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B; can replace Mg2+ with lower relative activity
Mn2+
Q3HW35
the enzyme requires Mn2+ or Mg2+, which are equally active
Mn2+
-
can partially replace Mg2+ in activation, inhibition in presence of Mg2+
NH4+
-
activity is dependent on both divalent Mg2+ or Mn2+ and monovalent cations NH4+ or K+
additional information
-
divalent cations are required for tripolyphosphatase activity
additional information
-
no stimulatory effect by K+ has been observed even at high concentration (40 mM)
additional information
no stimulatory effect by K+ has been observed even at high concentration (40 mM)
additional information
-
Mg2+, H2PO4-, NH4-, and NO3- have no significant effect on enzyme activity at 5 mM
additional information
Q3HW35
Ag+, Ca2+, Fe2+, and Cu2+ cannot substitue for Mg2+ or Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-(3-(2-ethoxyphenyl)ureidoacetyl)-4-(2-methyl-5-nitrophenyl)semicarbazide
-
binding to adenosyl region of the active site
1-(4-chloro-2-nitrophenyl)-3-(4-sulfamoylphenyl)-urea
-
binding to adenosyl region of the active site
2-amino-6-carboxyethylmercaptopurine
10 mM, 31.9% inhibition
2-amino-6-chloropurine-9-acetic acid
10 mM, 23.5% inhibition
5-amino-L-norvaline
10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM
5-azacytidine
-
0.2 mM leads to significant reduction of AdoMetS protein expression
6-dimethylallylaminopurine riboside
10 mM, 41.6% inhibition
ADP
35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM
carbon tetrachloride
-
depletion of glutathione levels reduces MAT I/III activities in vivo
ethanol
25 mM ethanol substantially decreases the enzymatic activity of MAT II
Ethionine
32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM
methanol
2.4% methanol depresses methionine adenosyltransferase specific activity, this effect is not observed with 0.8% methanol
nitrosoglutathione
-
reversibly inhibits the isozyme MAT1 via NO binding to Cys114, no inhibition of isozymes MAT2 and MAT3, molecular mechanism for S-nitrosylation of the enzyme
putrescine
15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM
S-nitrosylated glutathione
-
rapid and dose-dependent loss of enzymatic activity of MAT I/III
spermidine
15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM
spermine
30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM
ATP
-
ATP and methionine act as a switch between two different MAT III isoforms
bacterial lipopolysaccharide
-
results in the accumulation of nitrites and nitrates in serum and in the inactivation of MAT I/III
-
CTP
60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM
CTP
-
; 20 mM, 37% inhibition, S-adenosylmethionine synthetase B; 20 mM, 40% inhibition, S-adenosylmethionine synthetase A
Cu2+
isozyme subunit MATalpha2 is inhibited by 0.25 mM Cu2+ in the presence or absence of dithiothreitol, strong reduction in MAT2B gene expression induced by Cu2+ (60%), copper effects can only be prevented by buthionine sulfoximine, whereas N-acetylcysteine and neocuproine are ineffective
cycloleucine
1-aminocyclopentane-1-carboxylic acid, specific MAT inhibitor
cycloleucine
-
25 mM, 56% inhibition, S-adenosylmethionine synthetase A; inhibits only at sub saturating concentrations of methionine
diphosphate
-
individually a weak inhibitor, in combination with phosphate there is a marked synergistic effect
diphosphate
-
; 20 mM, 30% inhibition, S-adenosylmethionine synthetase A; 20 mM, 49% inhibition, S-adenosylmethionine synthetase B
GTP
not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM
GTP
-
competitive with respect to ATP and noncompetitive with L-methionine
GTP
-
; 20 mM, 50% inhibition, S-adenosylmethionine synthetase B; 20 mM, 56% inhibition, S-adenosylmethionine synthetase A
hydrogen peroxide
-
inactives CHO cells-MAT, prevented by desferoxamine. Time- and dose-dependent inactivation of MAT I/III, activity recovered by addition of glutathione
L-buthionine-(S,R)-sulfoximine
-
inhibits glutathione synthesis and this decreases MAT activity in vivo. Prevented by the administration of glutathione-ethyl ester
L-buthionine-(S,R)-sulfoximine
-
inactivates hepatic MAT, prevented by the administration of glutathione-ethyl ester
L-ethionine
-
1.2 mM leads to significant reduction of AdoMetS protein expression
L-ethionine
-
competitive with respect to methionine for S-adenosylmethionine formation and noncompetitive with respect to ATP
L-methionine
-
ATP and methionine act as a switch between two different MAT III isoforms
L-methionine
30% reduction in total activity is detected at 5 mM L-methionine; 30% reduction in total activity is detected at 5 mM L-methionine; 30% reduction in total activity is detected at 5 mM L-methionine
nitric oxide
-
two MAT III isoforms, one with low tripolyphosphatase activity that is insensitive to NO and another with high tripolyphosphatase activity that is inhibited by NO
p-chloromercuribenzoate
-
alpha and beta isoenzymes completely inhibited, gamma isoenzyme slightly inhibited
phosphate
-
individually a weak inhibitor, in combination with diphosphate there is a synergistic inhibitory effect
phosphate
-
; 10 mM, 19% inhibition, S-adenosylmethionine synthetase B; 10 mM, 45% inhibition, S-adenosylmethionine synthetase A
S-adenosylmethionine
-
noncompetitive inhibitor with respect to ATP and methionine
S-adenosylmethionine
-
inhibition of rat kidney enzyme and rat liver MAT-II, weak inhibition of rat liver MAT-I
S-adenosylmethionine
-
above 0.3 mM inhibits both high-MW and low-MW isoenzymes
S-adenosylmethionine
-
inhibits the A form but not the B form; non-competitive, S-adenosylmethionine synthetase A; slight inhibition of S-adenosylmethionine synthetase B
Tetrapolyphosphate
-
; 10 mM, 40% inhibition, S-adenosylmethionine synthetase B; 10 mM, 50% inhibition, S-adenosylmethionine synthetase A
tripolyphosphate
strong inhibitor; strong inhibitor; strong inhibitor
tripolyphosphate
-
competitive with ATP; non competitive with L-methionine
tripolyphosphate
-
activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration
tripolyphosphate
-
1.0 mM, 57% inhibition, S-adenosylmethionine synthetase A; 1.0 mM, 62% inhibition, S-adenosylmethionine synthetase B
additional information
-
addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect
-
additional information
addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect
-
additional information
addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect
-
additional information
addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect
-
additional information
-
overview of the regulatory properties, effect of L-methionine analogues and influence of L-methionine concentration on activating and inhibiting effects, effect of tripolyphosphate and p-hydroxymercuribenzoate
-
additional information
-
reactive oxygen and nitrogen species induce the inactivation of MAT I/III
-
additional information
-
no inhibition with cycloleucine, L-homocysteine, L-norleucine, L-cis-2-amino-4-methoxy-3-butenoic acid, S-adenosylhomocysteine, 5'-methylthioadenosine, sinefungin
-
additional information
-
not inhibitory: (R)-methioninol, 1,3,7-trimethyluric acid, 6-methylpurine
-
additional information
not inhibitory: (R)-methioninol, 1,3,7-trimethyluric acid, 6-methylpurine
-
additional information
-
MAT is inactivated after 6 h of incubation in hypoxia (3% O2) in rat hepatocytes, prevented by NG-monomethyl-L-arginine methyl ester. Hepatic MAT s a sensible target for free radicals in vivo
-
additional information
-
S-adenosyl(5')-3-methylthiopropylamine does not inhibit
-
additional information
-
overexpression of yeast AdoMet synthase plus cap guanine-N7 methyltransferase affords greater resistance to sinefungin than either enzyme alone
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
-
tripolyphosphatase activity stimulated by preincubation with ATP and methionine
cycloheximide
-
cycloheximide inhibition of protein synthesis increases isozyme MAT2 mRNA level in the logarithmic phase only, an indication that the gene is regulated in promastigotes at the posttranscriptional level by protein factors that targets the transcript for degradation
dithiothreitol
-
required for alpha and beta isoenzymes activity, not required for gamma enzyme
E2F1
-
present continually in the MAT2A promoter during liver regeneration
-
E2F3
-
present continually in the MAT2A promoter during liver regeneration
-
E2F4
-
present continually in the MAT2A promoter during liver regeneration
-
L-methionine
17% increase is observed at 60 mM L-methionine; 17% increase of activity is observed at 60 mM L-methionine
methanol
-
53.26% increased yield of S-adenosyl-L-methionine at 1.0% methanol
methionine
-
stimulation of activity demonstrated in vivo. Tripolyphosphatase activity stimulated by preincubation with ATP and methionine
n-heptane
-
53.26% increased yield of S-adenosyl-L-methionine at 1.0% n-heptane
sorbitol
-
53.26% increased yield of S-adenosyl-L-methionine at 1.2% sorbitol
Sp1
-
binding of Sp1 to the MAT2A promoter is essential for the transcriptional up-regulation of the gene
-
tripolyphosphates
-
activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration
Tumor necrosis factor alpha
-
induces expression of variant 1 (but not variant 2) MAT2beta
-
Dimethylsulfoxide
-
activates isoform from kidney and isoform MAT-III from liver
Dimethylsulfoxide
-
activates the beta isozyme from liver 13-15fold; weak activation of the alpha isozyme from liver
Epidermal growth factor
-
100 ng/ml upregulates MAT2A (but not MAT2beta) expression
Epidermal growth factor
Q3TH56
100 ng/ml upregulates MAT2A (but not MAT2beta) expression
insulin-like growth factor 1
-
100 ng/ml upregulates MAT2A (but not MAT2beta) expression
-
insulin-like growth factor 1
Q3TH56
100 ng/ml upregulates MAT2A (but not MAT2beta) expression
-
S-adenosylmethionine
-
nonessential activator of tripolyphosphatase activity in the range of 0.005-0.100 mM
S-adenosylmethionine
-
activates the tripolyphosphatase activity of A isoform
additional information
-
lysine enhances methionine content by modulating the expression of S-adenosylmethionine synthase, overview
-
additional information
-
salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview
-
additional information
salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview
-
additional information
salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview
-
additional information
salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview
-
additional information
salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview; salt stress induces the enzyme, differential effects on betaine and lignin biosynthesis, overview
-
additional information
cell methionine and S-adenosylmethionine contents increase in response to hyperoxia in SAE and A549 cells, enzyme activity increases by 2fold within 5 days, S-adenosylmethionine content by 5fold, overview
-
additional information
-
methanol induces enzyme expression and S-adenosyl-L-methionine production, while glycerol does not
-
additional information
-
fasting for 10-16 h increases expression and activity of isozymes MAT I and MAT III, effects on methionine/S-adenosyl-L-methionine metabolism, overview
-
additional information
-
no effect with ADP and methionine or S-adenosylmethionine
-
additional information
-
no significant effect in the presence of n-dodecane
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.11
ATP
-
mutant W387F, 55°C; mutant W387F/Y371W, 55°C; mutant W387F/Y72W, 55°C
0.01
L-methionine
-
isoenzyme A; S-adenosylmethionine synthetase A
0.022
L-methionine
one kinetic form of alpha-two subunit in the presence of beta subunit
0.03 - 0.038
L-methionine
-
one kinetic form of alpha subunit in the presence of beta subunit
0.038
L-methionine
-
mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.045
L-methionine
-
mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.05
L-methionine
-
mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.06
L-methionine
-
uninduced E. coli NM522 strain extract alpha subunit at low L-methionine concentrations
0.065 - 0.08
L-methionine
-
alpha subunit at low L-methionine concentrations
0.074
L-methionine
-
mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.076
L-methionine
one kinetic form of alpha-two subunit in the presence of beta subunit
0.08 - 0.09
L-methionine
-
alpha subunit at high L-methionine concentrations. Also one kinetic form of alpha subunit in the presence of beta subunit
0.08
L-methionine
-
induced E. coli NM522 strain extract alpha subunit at low L-methionine concentrations and uninduced E. coli NM522 strain extract alpha subunit at high L-methionine concentrations
0.13
L-methionine
-
mutant W387F/Y323W, 55°C; mutant W387F/Y72W, 55°C
0.137
L-methionine
-
mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0; mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.147
L-methionine
-
mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.17
L-methionine
-
mutant K256A, pH 8.0; mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.176
L-methionine
-
mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.2
L-methionine
-
induced E. coli NM522 strain extract alpha subunit at high L-methionine concentrations
0.287
L-methionine
-
wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.3
L-methionine
-
mutant W387F/Y226W, 55°C; mutant W387F/Y273W, 55°C
0.0013
tripolyphosphate
-
wild type, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.0015
tripolyphosphate
-
G7 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.0016
tripolyphosphate
-
G8 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.003
tripolyphosphate
-
wild type, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.0032
tripolyphosphate
-
G6 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.0053
tripolyphosphate
-
G5 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.008
tripolyphosphate
-
G8 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.011
tripolyphosphate
-
G6 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.014
tripolyphosphate
-
RLL mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.015
tripolyphosphate
-
G7 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.024
tripolyphosphate
-
G5 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.026
tripolyphosphate
-
RLL mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
additional information
additional information
-
high performance liquid chromatography assay method using catechol-O-methyltransferase-coupled fluorometric detection, negative cooperativity of enzyme with Hill coefficinet of 0.5
-
additional information
additional information
-
different isoforms of MAT differ in kinetic and regulatory properties, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.62
L-methionine
-
mutant W387F/Y323W, 55°C; mutant W387F/Y72W, 55°C
2.39
L-methionine
-
mutant W387F/Y170W, 55°C; mutant W387F/Y255W, 55°C
0.000000367
S-adenosylmethionine
-
RLL mutant, S-adenosylmethionine synthesis
0.000000667
S-adenosylmethionine
-
G8 mutant, S-adenosylmethionine synthesis
0.00000122
tripolyphosphate
-
RLL and G7 mutants, tripolyphosphatase activity
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
S-adenosylmethionine
-
inhibits the A isoform; S-adenosylmethionine synthetase A
4
S-adenosylmethionine
-
noncompetitive inhibition at 0.5-5 mM L-methionine concentrations
additional information
additional information
-
Ki values for several phosphonate analogues and nucleotides
-
additional information
additional information
-
Ki values for several phosphonate analogues and nucleotides
-
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