EC Number |
Metals/Ions |
Reference |
---|
5.4.99.16 | Mg2+ |
1 mM, slightly enhances the activity (10.9%) |
759622 |
5.4.99.16 | Mg2+ |
10 mM, 29% increase in activity |
681153 |
5.4.99.16 | Mg2+ |
112% activity at 1 mM |
715078 |
5.4.99.16 | Mg2+ |
118% relative activity at 1 mM |
701844 |
5.4.99.16 | Mg2+ |
8% activating at 10 mM |
748015 |
5.4.99.16 | Mg2+ |
activates |
748492 |
5.4.99.16 | Mg2+ |
hexa-coordinated magnesium ion located in the solvent-accessible loop L1, residues 50-60, of domain A, about 25 A from the catalytic center, enzyme binding structure, overview. Interactions with this magnesium ion come from Asp51, Asn53, Asp55, Ile57, Asp59 and one water molecule |
727598 |
5.4.99.16 | Mg2+ |
the Mg2+ site consists of the side chains of Asp24, Asn26, Asp28 and Asp32 and the main-chain O atom of Lys30, which form part of the consensus signature DXNXDGXGD. Such a site is also found in some other GH13 member |
746621 |
5.4.99.16 | Mn2+ |
116% relative activity at 1 mM |
701844 |
5.4.99.16 | Mn2+ |
8% activating at 10 mM |
748015 |