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Literature summary for 5.4.99.16 extracted from

  • Al Faik, M.; Das, R.; Bo, J.; Mu, W.; Hassanin, H.
    Cloning, purification and characterization of a novel recombinant trehalose synthase (TreS) from Acidiplasma sp. MBA-1 (2019), J. Microbiol. Biotechnol. Food Sci., 8, 1298-1302 .
No PubMed abstract available
Search for more literature for 5.4.99.16

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, slightly enhances the activity (9.6%) Acidiplasma sp. MBA-1

Application

Application Comment Organism
synthesis the enzyme (TreS) produced from Acidiplasma spMBA-1 can catalyze a considerable amount of maltose into trehalose in a single step reaction. Maltose is a relatively cheap substrate. Hence, TreS can be used as an alternative commercial enzyme to produce trehalose commercially. A significant amount of glucose is being produced as a byproduct which hinders the production of commercial trehalose. If it is possible to suppress glucose production by genetic modifications, it could enhance trehalose production Acidiplasma sp. MBA-1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE) Acidiplasma sp. MBA-1

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, 38% loss of activity Acidiplasma sp. MBA-1
Cu2+ 1 mM, complete inhibition Acidiplasma sp. MBA-1
Ni2+ 1 mM, 58% loss of activity Acidiplasma sp. MBA-1
Zn2+ 1 mM, 67% loss of activity Acidiplasma sp. MBA-1

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ 1 mM, slightly enhances the activity (6.7%) Acidiplasma sp. MBA-1
Mg2+ 1 mM, slightly enhances the activity (10.9%) Acidiplasma sp. MBA-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65900
-
 SDS-PAGE Acidiplasma sp. MBA-1

Organism

Organism UniProt Comment Textmining
Acidiplasma sp. MBA-1 A0A0D8DNG2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acidiplasma sp. MBA-1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.57
-
 pH and temperature not specified in the publication Acidiplasma sp. MBA-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose
-
 Acidiplasma sp. MBA-1 maltose
-
 ?
maltose conversion of trehalose from maltose is 43.62%. At the same time, TreS produces about 23.85% glucose as a by product after 10 h of incubation Acidiplasma sp. MBA-1 alpha,alpha-trehalose
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Acidiplasma sp. MBA-1

Synonyms

Synonyms Comment Organism
TreS
-
 Acidiplasma sp. MBA-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Acidiplasma sp. MBA-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 25°C: about 40% of maximal activity, 55°C: about 60% of maximal activity, recombinant enzyme Acidiplasma sp. MBA-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, stable Acidiplasma sp. MBA-1
45
-
 1 h, about 10% loss of activity Acidiplasma sp. MBA-1
50
-
 1 h, about 30% loss of activity Acidiplasma sp. MBA-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant enzyme Acidiplasma sp. MBA-1

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 80% of maximal activity, pH 7.5: about 50% of maximal activity Acidiplasma sp. MBA-1