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Literature summary for 5.4.99.16 extracted from

  • Wang, T.; Dai, K.; Liu, H.; Jia, S.; Wang, R.
    Cloning and expression of a trehalose synthase from Pseudomonas putida KT2440 in Bacillus subtilis W800N for the production of trehalose (2014), J. Pure Appl. Microbiol., 8, 1687-1692 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene treSB, DNA and amino acid sequence determination and analysis, recombinant expression in Bacillus subtilis strain W800N, recombinant expression of His6-tagged enzyme in Escherichia coli strain DH5alpha Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Pseudomonas putida
Zn2+
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Pseudomonas putida
Mg2+ activates Pseudomonas putida
Na+ activates Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose Pseudomonas putida
-
alpha,alpha-trehalose
-
r
maltose Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440
-
alpha,alpha-trehalose
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88FN0
-
-
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 Q88FN0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
Pseudomonas putida alpha,alpha-trehalose
-
r
maltose
-
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 alpha,alpha-trehalose
-
r

Synonyms

Synonyms Comment Organism
Trehalose synthase
-
Pseudomonas putida
TreS
-
Pseudomonas putida
treSB
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
recombinant His6-tagged enzyme Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant His6-tagged enzyme Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 purified recombinant His6-tagged enzyme, stable at Pseudomonas putida