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maltose = alpha,alpha-trehalose
maltose = alpha,alpha-trehalose
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maltose = alpha,alpha-trehalose
two-step, double displacement catalytic mechanism, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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maltose = alpha,alpha-trehalose
two-step, double displacement mechanism of the enzyme, overview
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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alpha,alpha-trehalose
maltose
high-maltose rice syrup
alpha,alpha-trehalose
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the highest alpha,alpha-trehalose yield (54.6%) can be obtained by 3.5 units/maltose (g) of trehalose synthase from the maltose syrup at 50°C for 20-24 h
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?
maltose
alpha,alpha-trehalose
maltose
alpha,alpha-trehalose + D-glucose + maltose
starch
alpha,alpha-trehalose
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substrate of recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa. Catalytic efficiency of fusion protein is higher than that of a mixture of individual enzymes
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?
sucrose
D-glucose + D-fructose + [alpha-D-glucopyranosyl-(1,1)-D-fructofuranose]
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low activity on sucrose
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?
sucrose
trehalulose
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activity is very low compared to that with maltose
i.e. 1-O-alpha-D-glucopyranosyl-D-fructose
?
additional information
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alpha,alpha-trehalose
maltose
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?
alpha,alpha-trehalose
maltose
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r
alpha,alpha-trehalose
maltose
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r
alpha,alpha-trehalose
maltose
-
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?
alpha,alpha-trehalose
maltose
-
ratio of kcat to Km value is 2.5fold higher for maltose than for trehalose
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r
alpha,alpha-trehalose
maltose
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?
alpha,alpha-trehalose
maltose
composition of the TtTreS active site is studied through computer calculation and enzyme analysis. The results are consistent with a two-step double-displacement mechanism. The data suggest that glucose rotation, following breakage of the alpha-1,4 glycosidic bond, is a key factor determining the reaction direction and conversion rate. The N246 residue plays an important role in glucose rotation
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r
alpha,alpha-trehalose
maltose
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r
alpha,alpha-trehalose
maltose
-
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r
alpha,alpha-trehalose
maltose
WP_028494267
1.48fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose
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r
alpha,alpha-trehalose
maltose
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r
alpha,alpha-trehalose
maltose
-
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?
alpha,alpha-trehalose
maltose
-
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r
alpha,alpha-trehalose
maltose
-
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?
maltose
alpha,alpha-trehalose
conversion of trehalose from maltose is 43.62%. At the same time, TreS produces about 23.85% glucose as a by product after 10 h of incubation
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?
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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the maximum conversion yield reaches 69% at 25°C after 9 h of reaction
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r
maltose
alpha,alpha-trehalose
-
the maximum conversion yield reaches 69% at 25°C after 9 h of reaction
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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-
r
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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wild-type, 92% yield
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
the enzyme has a 2fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose indicating maltose as the preferred substrate
TreS also has a weak hydrolytic property with D-glucose as the byproduct
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r
maltose
alpha,alpha-trehalose
an enzymatic intramolecular rearrangement reaction
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r
maltose
alpha,alpha-trehalose
the enzyme has a 2fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose indicating maltose as the preferred substrate
TreS also has a weak hydrolytic property with D-glucose as the byproduct
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
an enzymatic intramolecular rearrangement reaction
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r
maltose
alpha,alpha-trehalose
bioconversions results in final trehalose levels of 60%. The enzyme produces reduced amounts of the byproduct glucose (15%)
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
two-step, double-displacement mechanism
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
two-step, double-displacement mechanism
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
-
as a byproduct, about 13% glucose is also produced
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r
maltose
alpha,alpha-trehalose
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as a byproduct, about 13% glucose is also produced
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r
maltose
alpha,alpha-trehalose
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
-
ratio of kcat to Km value is 2.5fold higher for maltose than for trehalose. Maximum conversion rate for maltose into trehalose is independent of substrate concentration and reaches 71% at 20°C
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r
maltose
alpha,alpha-trehalose
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one-step conversion via intramolecular transglucosylation reaction
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?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
-
?
maltose
alpha,alpha-trehalose
-
r
-
?
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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30% yield
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?
maltose
alpha,alpha-trehalose
conversion of 59% maltose by the purified recombinant enzyme with about 5.1% D-glucose as by-product
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
conversion of 59% maltose by the purified recombinant enzyme with about 5.1% D-glucose as by-product
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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r
maltose
alpha,alpha-trehalose
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5.4.99.16 maltose alpha-D-glucosyltransferase
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