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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
2.4.2.29
0.00012
-
tRNA
histidine-tagged wild-type enzyme, pH 7.3
644933
2.4.2.29
0.00039
-
tRNA
histidine-tagged wild-type enzyme, pH 8.5
644933
2.4.2.29
0.00051
-
tRNA
histidine-tagged mutant enzyme D89E, pH 8.5
644933
2.4.2.29
0.0009
-
tRNA guanine
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
688390
2.4.2.29
0.001
-
tRNA guanine
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
688390
2.4.2.29
0.00032
-
tRNA(Tyr)
wild-type enzyme
644934
2.4.2.29
0.0097
-
tRNA(Tyr)
mutant enzyme S90C
644934
2.4.2.29
0.00087
-
virF minihelix RNA
at pH 7.3
689250
2.4.2.29
0.0018
-
virF mRNA
at pH 7.3
689250
2.4.2.29
0.00262
-
[RNAECYMA]-2'-deoxyguanine 34
-
702263
Results
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