EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.14.16.2 | 0.00035 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine |
telencephalon extract, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C |
673820 |
1.14.16.2 | 0.00045 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine |
hypothalamus extract, 0.01 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C |
673820 |
1.14.16.2 | 0.00048 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine |
telencephalon extract, 0.01 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C |
673820 |
1.14.16.2 | 0.00054 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine |
telencephalon extract, 1 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C |
673820 |
1.14.16.2 | 0.00058 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine |
hypothalamus extract, 1 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C |
673820 |
1.14.16.2 | 0.00105 |
- |
L-tyrosine |
mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C |
727014 |
1.14.16.2 | 0.00125 |
- |
phenylalanine |
recombinant mutant D425V |
438709 |
1.14.16.2 | 0.0016 |
- |
L-phenylalanine |
mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C |
727014 |
1.14.16.2 | 0.0041 |
- |
tetrahydrobiopterin |
pH 7.0, 37°C |
716647 |
1.14.16.2 | 0.005 |
- |
L-tyrosine |
pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.3 mM tetrahydropterin |
657679 |