Protein Variants | Comment | Organism |
---|---|---|
S19E | similar steady-state parameters and similar binding affinity for catecholamines to wild-type enzyme. Inactivated 1.8fold slower than the wild-type enzyme at 42°C in presence of 2% glycerol | Rattus norvegicus |
S31E | similar steady-state parameters and similar binding affinity for catecholamines to wild-type enzyme. Inactivated 1.8fold slower than the wild-type enzyme at 42°C in presence of 2% glycerol | Rattus norvegicus |
S40E | inactivated 1.6fold faster than the wild-type enzyme at 42°C in presence of 2% glycerol | Rattus norvegicus |
S8E | similar steady-state parameters and similar binding affinity for catecholamines to wild-type enzyme. More stable than wild-type enzyme at 42°C in presence of 2% glycerol | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.008 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.009 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.009 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.009 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.009 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.009 | - |
L-tyrosine | pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.01 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.011 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.011 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.3 mM tetrahydropterin | Rattus norvegicus | |
0.023 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S19E/S40E | Rattus norvegicus | |
0.026 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.028 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S8E | Rattus norvegicus | |
0.032 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S19A | Rattus norvegicus | |
0.033 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S19A | Rattus norvegicus | |
0.033 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S19E | Rattus norvegicus | |
0.034 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, wild-type enzyme | Rattus norvegicus | |
0.035 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.035 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S40A | Rattus norvegicus | |
0.035 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S40E | Rattus norvegicus | |
0.035 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S8E | Rattus norvegicus | |
0.036 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S8A | Rattus norvegicus | |
0.037 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S19E | Rattus norvegicus | |
0.037 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S19E/S40E | Rattus norvegicus | |
0.037 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S40E | Rattus norvegicus | |
0.039 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S8A | Rattus norvegicus | |
0.04 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, wild-type enzyme | Rattus norvegicus | |
0.041 | - |
L-tyrosine | pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.042 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.045 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S40A | Rattus norvegicus | |
0.046 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.047 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S31E | Rattus norvegicus | |
0.047 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.047 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.048 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S31A | Rattus norvegicus | |
0.051 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S31A | Rattus norvegicus | |
0.051 | - |
(RS)-6-methyl-5,6,7,8-tetrahydropterin | pH 7.0, 30°C, mutant enzyme S31E | Rattus norvegicus | |
0.053 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus | |
0.055 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.4 mM 6-0.053 methyltetrahydropterin | Rattus norvegicus | |
0.059 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-tyrosine + tetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
or below, all mutant enzymes are stable for at least 1 h | Rattus norvegicus |
45 | - |
in presence of 2% glycerol, enzymes lose activity in time-dependent manner | Rattus norvegicus |
50 | - |
in presence of 10% glycerol, all mutant enzymes are stable for at least 1 h | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
(RS)-6-methyl-5,6,7,8-tetrahydropterin | - |
Rattus norvegicus | |
tetrahydrobiopterin | - |
Rattus norvegicus |