1.14.16.2 additional information - additional information - 438654, 438696, 438697, 438698 1.14.16.2 additional information - additional information both the cofactor (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin and the feedback inhibitor dopamine increase the kinetic stability of human isozyme 1 in vitro 688521 1.14.16.2 additional information - additional information effect of RNA on Km 438683 1.14.16.2 additional information - additional information kinetics analysis, overview 688519 1.14.16.2 additional information - additional information Km dependence on substrate concentrations 438687, 438695 1.14.16.2 additional information - additional information Km dependent on cosubstrate type and concentration, overview for 4 isoforms 438705 1.14.16.2 additional information - additional information Km is dependent on substrate and cofactor concentration when (6R)-L-erythro-tetrahydrobiopterin is used due to inhibitory effects 438694 1.14.16.2 additional information - additional information Km-values for tyrosine of mutant phenylalanine hydroxylase with tyrosine hydroxylation activity 438709 1.14.16.2 additional information - additional information Km-values of diverse tetrahydropterin analogues, substituted at C6, C7 or C3 438710 1.14.16.2 additional information - additional information phosphorylation converts the enzyme from a form possessing a high Km for pterin cofactor to a form with a low Km for pterin cofactor 438690 1.14.16.2 additional information - additional information rat brain: 2 kinetically distinguishable forms: low Km form, high Km form 438683, 438690 1.14.16.2 0.0001 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 10 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00015 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 1 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.0002 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 10 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.0002 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 1 nM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00022 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 1 nM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00024 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 1 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00024 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 1 fM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00026 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 1 fM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00034 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00035 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00045 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 0.01 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00048 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 0.01 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00054 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine telencephalon extract, 1 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00058 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine hypothalamus extract, 1 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C 673820 1.14.16.2 0.00105 - L-tyrosine mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.00125 - phenylalanine recombinant mutant D425V 438709 1.14.16.2 0.0016 - L-phenylalanine mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0041 - tetrahydrobiopterin pH 7.0, 37°C 716647 1.14.16.2 0.005 - L-tyrosine pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.0053 - L-phenylalanine mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0056 - tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C 714235 1.14.16.2 0.006 - L-tyrosine mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.006 - O2 below, recombinant enzyme 438692 1.14.16.2 0.0061 - tyrosine - 438683 1.14.16.2 0.0068 - tetrahydrobiopterin wild type protein, dopamine bound but removed from low-affinity site, pH 7.2, 30°C 714235 1.14.16.2 0.0069 - L-tyrosine mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0072 - tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.0077 - phenylalanine recombinant mutant H323Y 438709 1.14.16.2 0.008 - L-tyrosine pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.0081 - L-tyrosine mutant enzyme R37E/R38E, pH and temperature not specified in the publication 727798 1.14.16.2 0.0083 - tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.0088 - phenylalanine recombinant mutant Q310H 438709 1.14.16.2 0.0089 - L-tyrosine mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.009 - L-tyrosine pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.009 - L-tyrosine pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.009 - L-tyrosine pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.009 - L-tyrosine pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.009 - L-tyrosine pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.009 - tetrahydrobiopterin pH 7.0, 30°C, mutant enzyme T463M 660477 1.14.16.2 0.0092 - L-phenylalanine mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0094 - tyrosine recombinant enzyme, pH-independent 438692 1.14.16.2 0.0095 - tyrosine - 438707 1.14.16.2 0.0099 - tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.01 - L-tyrosine pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.01 - phenylalanine recombinant mutant Y371F 438711 1.14.16.2 0.01 - tetrahydrobiopterin pH 7.0, 30°C, mutant enzyme R306H 660477 1.14.16.2 0.01 - tetrahydrobiopterin pH 7.0, 30°C, mutant enzyme T283M 660477 1.14.16.2 0.01 - Tetrahydropterin recombinant phosphorylated hTH1, L-dopa-oxidase activity 438706 1.14.16.2 0.0105 - L-phenylalanine mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0106 - L-tyrosine mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.011 - L-tyrosine pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.011 - L-tyrosine pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.3 mM tetrahydropterin 657679 1.14.16.2 0.011 - tyrosine - 438703 1.14.16.2 0.012 - tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.013 - tetrahydrobiopterin pH 7.0, 30°C, wild-type enzyme 660477 1.14.16.2 0.014 - L-tyrosine mutant enzyme K366L, pH and temperature not specified in the publication 727798 1.14.16.2 0.014 - tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.014 - tetrahydrobiopterin pH 7.0, 30°C, mutant enzyme T245P 660477 1.14.16.2 0.014 - tetrahydrobiopterin wild type protein, pH 7.2, 30°C 714235 1.14.16.2 0.0144 - L-phenylalanine mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0148 - L-phenylalanine mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.015 - L-tyrosine mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.015 - L-tyrosine mutant enzyme E362R/E365R, pH and temperature not specified in the publication 727798 1.14.16.2 0.015 - tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.0152 - L-phenylalanine mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.016 - L-tyrosine mutant enzyme D361N, pH and temperature not specified in the publication 727798 1.14.16.2 0.016 - L-tyrosine mutant enzyme E362G, pH and temperature not specified in the publication 727798 1.14.16.2 0.016 - L-tyrosine wild type enzyme, pH and temperature not specified in the publication 727798 1.14.16.2 0.016 - tyrosine recombinant wild-type 438709 1.14.16.2 0.0164 - L-phenylalanine mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.017 - L-tyrosine 0-0.4 mM L-tyrosine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C 676182 1.14.16.2 0.017 - tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.017 - tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.0172 - L-tyrosine mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0187 - L-tyrosine mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.019 - L-phenylalanine mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.019 - L-tyrosine mutant enzyme E365G, pH and temperature not specified in the publication 727798 1.14.16.2 0.02 - L-tyrosine mutant enzyme A297L, pH and temperature not specified in the publication 727798 1.14.16.2 0.02 - tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.021 - L-phenylalanine mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.021 - Tetrahydropterin recombinant enzyme, value is pH-dependent 438692 1.14.16.2 0.022 - L-phenylalanine mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.023 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E/S40E 657679 1.14.16.2 0.024 - L-tyrosine W166F/W233F/W372F/F14W mutant protein, pH 7, 25°C 714239 1.14.16.2 0.025 - L-tyrosine mutant enzyme S368A, pH and temperature not specified in the publication 727798 1.14.16.2 0.026 - L-tyrosine pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.026 - tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.0263 - L-tyrosine recombinant wild type enzyme, at pH 7.5 and 37°C 728715 1.14.16.2 0.027 - tetrahydrobiopterin A297L mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C 714235 1.14.16.2 0.027 - tetrahydrobiopterin Y371F mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C 714235 1.14.16.2 0.027 - Tetrahydropterin recombinant wild-type, pH 7.1 438711 1.14.16.2 0.028 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8E 657679 1.14.16.2 0.028 - L-phenylalanine mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.029 - L-tyrosine native wild type enzyme, at pH 7.5 and 37°C 728715 1.14.16.2 0.029 - tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.029 - tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.03 - L-tyrosine mutant enzyme Y423A, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0308 - L-tyrosine mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.032 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19A 657679 1.14.16.2 0.032 - tyrosine recombinant wild-type, pH 6.0 438711 1.14.16.2 0.033 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19A 657679 1.14.16.2 0.033 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E 657679 1.14.16.2 0.033 - 6-Methyl-5,6,7,8-tetrahydropterin recombinant wild-type, pH 7.1 438711 1.14.16.2 0.034 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, wild-type enzyme 657679 1.14.16.2 0.034 - tetrahydrobiopterin E332D mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C 714235 1.14.16.2 0.035 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40A 657679 1.14.16.2 0.035 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40E 657679 1.14.16.2 0.035 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8E 657679 1.14.16.2 0.035 - L-tyrosine pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.036 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8A 657679 1.14.16.2 0.037 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E 657679 1.14.16.2 0.037 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E/S40E 657679 1.14.16.2 0.037 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40E 657679 1.14.16.2 0.039 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8A 657679 1.14.16.2 0.039 - L-tyrosine pH 7.0, 30°C, mutant enzyme T283M 660477 1.14.16.2 0.04 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, wild-type enzyme 657679 1.14.16.2 0.04 - L-tyrosine 30°C 658785 1.14.16.2 0.04 - L-tyrosine mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.04 - L-tyrosine wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.04 - L-tyrosine wild type protein, pH 7, 25°C 714239 1.14.16.2 0.04 - tetrahydrobiopterin wild type protein, pH 7, 25°C 714239 1.14.16.2 0.041 - L-tyrosine mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.041 - L-tyrosine pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.042 - L-tyrosine pH 7.0, 30°C, mutant enzyme R306H 660477 1.14.16.2 0.042 - L-tyrosine pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.0426 - tetrahydrobiopterin pH 6.9, 37°C 716428 1.14.16.2 0.0428 - L-tyrosine mutant enzyme E434A, at pH 7.5 and 37°C 728715 1.14.16.2 0.043 - L-tyrosine pH 7.0, 30°C, mutant enzyme T463M 660477 1.14.16.2 0.043 - L-tyrosine W166F/W233F/W372F/F74W mutant protein, pH 7, 25°C 714239 1.14.16.2 0.044 - L-tyrosine mutant enzyme Q424A, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.045 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40A 657679 1.14.16.2 0.045 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine 0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C 672211 1.14.16.2 0.045 - L-phenylalanine mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.045 - tetrahydrobiopterin - 438707 1.14.16.2 0.045 - tyrosine recombinant mutant D425V 438709 1.14.16.2 0.046 - L-tyrosine pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.046 - L-tyrosine pH 7.0, 30°C, wild-type enzyme 660477 1.14.16.2 0.047 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31E 657679 1.14.16.2 0.047 - L-tyrosine pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.047 - L-tyrosine pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.0471 - tetrahydrobiopterin native wild type enzyme, at pH 7.5 and 37°C 728715 1.14.16.2 0.048 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31A 657679 1.14.16.2 0.05 - tyrosine bovine adrenal gland 438686 1.14.16.2 0.051 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31A 657679 1.14.16.2 0.051 - (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31E 657679 1.14.16.2 0.051 - 6-methyltetrahydropterin pH 7.1, 25°C, wild-type enzyme 657979 1.14.16.2 0.051 - L-Tyr pH 7.1, 25°C, wild-type enzyme 657979 1.14.16.2 0.051 - L-tyrosine mutant enzyme T427A, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.051 - tyrosine recombinant wild-type, pH 7.1 438711 1.14.16.2 0.0519 - tetrahydrobiopterin recombinant wild type enzyme, at pH 7.5 and 37°C 728715 1.14.16.2 0.053 - L-tyrosine pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.053 - Tetrahydropterin recombinant mutant Y371F, pH 7.1 438711 1.14.16.2 0.054 - tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.054 - tyrosine recombinant mutant Q310H 438709 1.14.16.2 0.055 - 6-Methyl-5,6,7,8-tetrahydropterin recombinant wild-type, pH 6.0 438711 1.14.16.2 0.055 - L-tyrosine pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.4 mM 6-0.053 methyltetrahydropterin 657679 1.14.16.2 0.055 - tetrahydrobiopterin W166F/W233F/W372F/F14W mutant protein, pH 7, 25°C 714239 1.14.16.2 0.055 - tyrosine recombinant isoform hTH2, with 6-methyltetrahydropterin 438705 1.14.16.2 0.056 - L-Dopa recombinant hTH1, dopa-oxidase activity with tetrahydropterin 438706 1.14.16.2 0.056 - L-tyrosine mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.0565 - L-phenylalanine mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.058 - 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine - 438688, 438698 1.14.16.2 0.058 - L-tyrosine W166F/W233F/W372F/F34W mutant protein, pH 7, 25°C 714239 1.14.16.2 0.059 - 6-Methyl-5,6,7,8-tetrahydropterin recombinant mutant Y371F, pH 7.1 438711 1.14.16.2 0.059 - L-tyrosine pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin 657679 1.14.16.2 0.06 0.1 6-Methyl-5,6,7,8-tetrahydropterin - 438703 1.14.16.2 0.062 - L-phenylalanine mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.065 - 6-Methyl-5,6,7,8-tetrahydropterin recombinant mutant Y371F, pH 6.0 438711 1.14.16.2 0.065 - tetrahydrobiopterin W166F/W233F/W372F/F74W mutant protein, pH 7, 25°C 714239 1.14.16.2 0.065 - tyrosine recombinant mutant Y371F, pH 7.1 438711 1.14.16.2 0.066 - L-tyrosine mutant enzyme Q426A, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.066 - L-tyrosine pH 7.0, 30°C, mutant enzyme T245P 660477 1.14.16.2 0.066 - tyrosine recombinant isoform hTH4, with 6-methyltetrahydropterin 438705 1.14.16.2 0.07 - tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C 714235 1.14.16.2 0.071 - tyrosine recombinant mutant Y371F, pH 6.0 438711 1.14.16.2 0.074 - tyrosine recombinant isoform hTH3, with 6-methyltetrahydropterin 438705 1.14.16.2 0.075 - tyrosine with 6-methyltetrahydropterin 438690 1.14.16.2 0.077 - tetrahydrobiopterin W166F/W233F/W372F/F34W mutant protein, pH 7, 25°C 714239 1.14.16.2 0.08 - L-tyrosine 0.38 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C 672211 1.14.16.2 0.08 - Tetrahydropterin low Km form 438683 1.14.16.2 0.085 - phenylalanine - 438703 1.14.16.2 0.088 - L-tyrosine mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.092 - tyrosine recombinant mutant H323Y 438709 1.14.16.2 0.097 - L-tyrosine mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.1 - L-phenylalanine wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.1 - phenylalanine recombinant wild-type 438709 1.14.16.2 0.11 - phenylalanine recombinant wild-type 438711 1.14.16.2 0.134 - tetrahydrobiopterin wild type protein, dopamine bound to the high-affinity site, pH 7.2, 30°C 714235 1.14.16.2 0.146 - L-Dopa recombinant, dopa-oxidase activity with 6,7-dimethyltetrahydropterin 438706 1.14.16.2 0.15 - 6-methyl-5,6,7,8-tetrahydrobiopterin - 438712 1.14.16.2 0.162 - L-tyrosine mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.166 - tyrosine recombinant isoform hTH1, with 6-methyltetrahydropterin 438705 1.14.16.2 0.172 - L-tyrosine mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.19 - 6-methyltetrahydropterin pH 7.1, 25°C, mutant enzyme H336E 657979 1.14.16.2 0.2 - L-phenylalanine mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 0.3 - phenylalanine bovine adrenal 438686 1.14.16.2 0.311 - L-Tyr pH 7.1, 25°C, mutant enzyme H336Q 657979 1.14.16.2 0.33 - L-Tyr pH 7.1, 25°C, mutant enzyme H336E 657979 1.14.16.2 0.339 - 6-methyltetrahydropterin pH 7.1, 25°C, mutant enzyme H331E 657979 1.14.16.2 0.34 - 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine 30°C 658785 1.14.16.2 0.341 - tetrahydrobiopterin mutant enzyme E434A, at pH 7.5 and 37°C 728715 1.14.16.2 0.356 - tetrahydrobiopterin wild type protein, in the presence of dopamine, pH 7.2, 30°C 714235 1.14.16.2 0.409 - 6-methyltetrahydropterin pH 7.1, 25°C, mutant enzyme H336Q 657979 1.14.16.2 0.5 - tyrosine - 438712 1.14.16.2 0.507 - tetrahydrobiopterin wild type, phosphorylated protein, in the presence of dopamine, pH 7.2, 30°C 714235 1.14.16.2 0.63 - Tetrahydropterin high Km form 438683 1.14.16.2 0.8086 - L-tyrosine pH 6.0, 37°C 716647 1.14.16.2 0.95 - 6-Methyl-5,6,7,8-tetrahydropterin - 438683 1.14.16.2 1.1 - tyramine pH 7.0, 37°C 716647 1.14.16.2 2.39 - L-tyrosine mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C 727014 1.14.16.2 5 - L-phenylalanine mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C 727014