EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.14.16.2 | 0.95 |
- |
6-Methyl-5,6,7,8-tetrahydropterin |
- |
438683 |
| 1.14.16.2 | 0.017 |
- |
L-tyrosine |
0-0.4 mM L-tyrosine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C |
676182 |
| 1.14.16.2 | 0.045 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine |
0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C |
672211 |
| 1.14.16.2 | 0.08 |
- |
L-tyrosine |
0.38 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C |
672211 |
| 1.14.16.2 | 0.04 |
- |
L-tyrosine |
30°C |
658785 |
| 1.14.16.2 | 0.34 |
- |
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine |
30°C |
658785 |
| 1.14.16.2 | 0.027 |
- |
tetrahydrobiopterin |
A297L mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C |
714235 |
| 1.14.16.2 | 0.02 |
- |
tetrahydrobiopterin |
A297L mutant protein, pH 7.2, 30°C |
714235 |
| 1.14.16.2 | 0.017 |
- |
tetrahydrobiopterin |
A297L phosphorylated mutant protein, pH 7.2, 30°C |
714235 |
| 1.14.16.2 | 0.006 |
- |
O2 |
below, recombinant enzyme |
438692 |
