Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) as maltose-binding protein fusion protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 80% inhibition at 0.1 mM | Homo sapiens | |
L-phenylalanine | - |
Homo sapiens | |
Mn2+ | 50% inhibition at 0.01 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
L-tyrosine | 0-0.4 mM L-tyrosine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98900 | - |
recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
one chromatography step | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine + O2 | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hTH2 | - |
Homo sapiens |
tyrosine hydroxylase | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.103 | - |
L-phenylalanine | 0-0.4 mM L-tyrosine, 0-0.2 mM L-phenylalanine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C | Homo sapiens |