EC Number   |
General Stability |
Reference |
|---|
    2.7.7.27 | bovine serum albumin stabilizes the purified enzyme at 0.5 mg/ml |
694734 |
| 2.7.7.27 | endosperm enzyme stability is increased by ammonium sulfate, as a supernatant of 30% saturing retains 50% of the activity after 5 min heating at 60°C, by additionally polyethylenegycol retains 70% of enzyme activity |
643160 |
| 2.7.7.27 | freeze-thawing reduces activity by 70%, high ionic strength, ATP and Mg2+ stabilize |
643119 |
| 2.7.7.27 | glycerol, 20% stabilizes |
643134 |
| 2.7.7.27 | irreversibly insoluble when dialyzed against deionized water |
643127 |
| 2.7.7.27 | no reducing reagent required during purification |
643133 |
| 2.7.7.27 | phosphate stabilizes |
643133 |
| 2.7.7.27 | phosphate, in the absence of 3-PGA, stabilizes endosperm AGPase |
694714 |
| 2.7.7.27 | PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA |
643120 |
| 2.7.7.27 | polyvinylpyrrolidone, i.e. PVP fractionation stabilizes during purification, 20% sucrose and 30 mM phosphate stabilize during purification |
643118 |
