Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | activation of leaf enzyme, however endosperm enzyme only, when phosphate, ADP or fructose 1,6-bisphosphate are present | Triticum aestivum | |
D-fructose 1,6-bisphosphate | activation of leaf enzyme, inhibition of endosperm enzyme | Triticum aestivum | |
D-fructose 6-phosphate | activation of leaf enzyme, no influence on endosperm enzyme | Triticum aestivum | |
phosphoenolpyruvate | - |
Triticum aestivum |
General Stability | Organism |
---|---|
endosperm enzyme stability is increased by ammonium sulfate, as a supernatant of 30% saturing retains 50% of the activity after 5 min heating at 60°C, by additionally polyethylenegycol retains 70% of enzyme activity | Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibition of endosperm enzyme, not leaf enzyme | Triticum aestivum | |
D-fructose 6-phosphate | only endosperm enzyme is inhibited, activation of leaf enzyme | Triticum aestivum | |
phosphate | phosphate inhibits leaf and endosperm enzymes, 3-phosphoglycerate and D-fructose 6-phosphate reverse endosperm inhibition | Triticum aestivum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Triticum aestivum | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
52000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
53000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
54000 | - |
leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
210000 | - |
endosperm enzyme | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | P12299 | wheat | - |
Purification (Comment) | Organism |
---|---|
- |
Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Triticum aestivum | - |
leaf | - |
Triticum aestivum | - |
seed | - |
Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the amount of enzyme activity is higher in endosperm than in leaves fresh tissue | Triticum aestivum |
2.4 | - |
endosperm enzyme | Triticum aestivum |
58.5 | - |
leaf enzyme | Triticum aestivum |
Storage Stability | Organism |
---|---|
-20°C, purified endosperm enzyme, 20% loss of activity after 1 month | Triticum aestivum |
-20°C, purified leaf enzyme, stable for at least 3 months | Triticum aestivum |
4°C, endosperm extract, 6-8 h, 50% loss of activity | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Triticum aestivum | diphosphate + ADP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | leaf: x * 51000 + x * 54000, endosperm: x * 52000 + x * 53000, SDS-PAGE | Triticum aestivum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
leaf enzyme remains stable after 5 min heating at 60°C, the heat treatment of endosperm enzyme results in 95% loss of activity | Triticum aestivum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
phosphate | pH 8.0, in absence of 0.25 mM 3-phosphoglycerate | Triticum aestivum | |
0.2 | - |
phosphate | leaf enzyme in absence of 3-phosphoglycerate | Triticum aestivum | |
0.7 | - |
phosphate | endosperm enzyme in absence of 3-phosphoglycerate | Triticum aestivum | |
0.91 | - |
phosphate | endosperm enzyme in presence of 0.25 mM 3-phosphoglycerate | Triticum aestivum | |
1.52 | - |
phosphate | endosperm enzyme in presence of 1 mM 3-phosphoglycerate | Triticum aestivum | |
2.49 | - |
phosphate | endosperm enzyme in presence of 5 mM 3-phosphoglycerate | Triticum aestivum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum aestivum | endosperm enzyme, isoelectric focusing | - |
6.1 |