Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | activation of mutant Mos(1-198) is reduced compared to the wild-type enzyme, overview | Zea mays | |
ADP-D-glucose | activation of mutant Mos(1-198) is reduced compared to the wild-type enzyme, overview | Solanum tuberosum | |
additional information | mutant Mos(1-198) naturally occurs in a semiactivated state in the absence of an activator | Zea mays | |
additional information | mutant Mos(1-198) naturally occurs in a semiactivated state in the absence of an activator | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
F332S | site-directed mutagenesis in the potato part of the chimeric mutant | Zea mays |
F332S | site-directed mutagenesis in the potato part of the chimeric mutant | Solanum tuberosum |
H341Y | site-directed mutagenesis in the potato part of the chimeric mutant | Zea mays |
H341Y | site-directed mutagenesis in the potato part of the chimeric mutant | Solanum tuberosum |
I323V | site-directed mutagenesis in the potato part of the chimeric mutant | Zea mays |
I323V | site-directed mutagenesis in the potato part of the chimeric mutant | Solanum tuberosum |
additional information | expression of the maize/potato small subunit mosaic mutant MP, Mos(1-198), containing the first 198 amino acids of the small subunit of the maize endosperm enzyme and the last 277 amino acids from the potato tuber enzyme, Mos(1-198) and its derivatives are expressed exclusively with the wild-type maize large subunit, SH2. In the absence of activator, performs like a wild-type AGPase that is partially activated with 3-phosphoglycerate, enzymatic activity in the absence of 3-phosphoglycerate is substantially 2-5fold higher than that of wild-type enzyme, while in presence of 3-phosphoglycerate, Mos(1-198) AGPase activity is actually less than that of wild-type enzyme, phenotype, mutant Mos(1-198) naturally occurs in a semiactivated state in the absence of an activator, overview. Mutational exchange of carboxylterminal region containing 15 polymorphic amino acids from 377 to 475 to create Mos(1-198, 430-475) and Mos(1-198, 377-429) leading to reduced enzyme activity independent of 3-phosphoglycerate. Constructed mutant Mos(1-277) exhibits 3-phosphoglycerate-independent activity that is less than mutant Mos(1-198) and wild-type activity. Mutant Mos(1-321) has no detectible activity in the absence of 3-phosphoglycerate. In the presence of 3-phosphoglycerate however, Mos(1-321) activity in the reverse direction is identical to that of Mos(1-277), overview | Zea mays |
additional information | expression of the maize/potato small subunit mosaic mutant MP, Mos(1-198), containing the first 198 amino acids of the small subunit of the maize endosperm enzyme and the last 277 amino acids from the potato tuber enzyme, Mos(1-198) and its derivatives are expressed exclusively with the wt maize large subunit, SH2. In the absence of activator, performs like a wild-type AGPase that is partially activated with ADP-D-glucose, enzymatic activity in the absence of 3-phosphoglycerate is substantially 2-5fold higher than that of wild-type enzyme, while in presence of 3-phosphoglycerate, Mos(1-198) AGPase activity is actually less than that of wild-type enzyme, phenotype, mutant Mos(1-198) naturally occurs in a semiactivated state in the absence of an activator, overview. Mutational exchange of carboxylterminal region containing 15 polymorphic amino acids from 377 to 475 to create Mos(1-198, 430-475) and Mos(1-198, 377-429) leading to reduced enzyme activity independent of 3-phosphoglycerate. Constructed mutant Mos(1-277) exhibits 3-phosphoglycerate-independent activity that is less than mutant Mos(1-198) and wild-type activity. Mutant Mos(1-321) has no detectible activity in the absence of 3-phosphoglycerate. In the presence of 3-phosphoglycerate however, Mos(1-321) activity in the reverse direction is identical to that of Mos(1-277), overview | Solanum tuberosum |
N369H | site-directed mutagenesis in the potato part of the chimeric mutant | Zea mays |
N369H | site-directed mutagenesis in the potato part of the chimeric mutant | Solanum tuberosum |
V347M | site-directed mutagenesis in the potato part of the chimeric mutant | Zea mays |
V347M | site-directed mutagenesis in the potato part of the chimeric mutant | Solanum tuberosum |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes the purified enzyme at 0.5 mg/ml | Zea mays |
bovine serum albumin stabilizes the purified enzyme at 0.5 mg/ml | Solanum tuberosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | mutant Mos(1-198) is significantly inhibited in the absence of 3-phosphoglycerate even at low phosphate concentrations. Inhibition levels of wild-type and deletion mutants by phosphate, overview | Solanum tuberosum | |
phosphate | mutant Mos(1-198) is significantly inhibited in the absence of 3-phosphoglycerate even at low Pi concentrations. Inhibition levels of wild-type and deletion mutants by phosphate, overview | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198), in absence of 3-phosphoglycerate | Zea mays | |
0.018 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198), in absence of 3-phosphoglycerate | Solanum tuberosum | |
0.039 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198, 430-475), in absence of 3-phosphoglycerate | Zea mays | |
0.039 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198, 430-475), in absence of 3-phosphoglycerate | Solanum tuberosum | |
0.04 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198, 377-429), in absence of 3-phosphoglycerate | Zea mays | |
0.04 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198, 377-429), in absence of 3-phosphoglycerate | Solanum tuberosum | |
0.04 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-376), in absence of 3-phosphoglycerate | Zea mays | |
0.04 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-376), in absence of 3-phosphoglycerate | Solanum tuberosum | |
0.044 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-277), in absence of 3-phosphoglycerate | Zea mays | |
0.044 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-277), in absence of 3-phosphoglycerate | Solanum tuberosum | |
0.051 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198), in presence of 3-phosphoglycerate | Zea mays | |
0.051 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198), in presence of 3-phosphoglycerate | Solanum tuberosum | |
0.056 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198, 377-475), in absence of 3-phosphoglycerate | Zea mays | |
0.056 | - |
ATP | pH 7.4, 37°C, mutant Mos(1-198, 377-475), in absence of 3-phosphoglycerate | Solanum tuberosum | |
0.074 | - |
ATP | pH 7.4, 37°C, wild-type enzyme, in presence of 3-phosphoglycerate | Zea mays | |
0.074 | - |
ATP | pH 7.4, 37°C, wild-type enzyme, in presence of 3-phosphoglycerate | Solanum tuberosum | |
0.075 | - |
ATP | pH 7.4, 37°C, wild-type enzyme, in absence of 3-phosphoglycerate | Zea mays | |
0.075 | - |
ATP | pH 7.4, 37°C, wild-type enzyme, in absence of 3-phosphoglycerate | Solanum tuberosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Zea mays | |
Mg2+ | - |
Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Zea mays | - |
ADP-D-glucose + diphosphate | - |
r | |
ATP + alpha-D-glucose 1-phosphate | Solanum tuberosum | - |
ADP-D-glucose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant AGPases using protamine sulfate and ammonium sulfate fractionation, followed by ion exchange and hydroxyapatite chromatography | Zea mays |
recombinant wild-type and mutant AGPases using protamine sulfate and ammonium sulfate fractionation, followed by ion exchange and hydroxyapatite chromatography | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
leaf | - |
Solanum tuberosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
purified mutant Mos(1-198, 377-475) in absence of 3-phosphoglycerate | Zea mays |
0.72 | - |
purified mutant Mos(1-198, 377-475) in absence of 3-phosphoglycerate | Solanum tuberosum |
1.47 | - |
purified mutant Mos(1-376) in absence of 3-phosphoglycerate | Zea mays |
1.47 | - |
purified mutant Mos(1-376) in absence of 3-phosphoglycerate | Solanum tuberosum |
1.68 | - |
purified mutant Mos(1-198, 430-475) in absence of 3-phosphoglycerate | Zea mays |
1.68 | - |
purified mutant Mos(1-198, 430-475) in absence of 3-phosphoglycerate | Solanum tuberosum |
1.78 | - |
purified mutant Mos(1-198, 377-429) in absence of 3-phosphoglycerate | Zea mays |
1.78 | - |
purified mutant Mos(1-198, 377-429) in absence of 3-phosphoglycerate | Solanum tuberosum |
1.8 | - |
purified mutant Mos(1-277) in absence of 3-phosphoglycerate | Zea mays |
1.8 | - |
purified mutant Mos(1-277) in absence of 3-phosphoglycerate | Solanum tuberosum |
2.45 | - |
purified wild-type enzyme, in absence of 3-phosphoglycerate | Zea mays |
2.45 | - |
purified wild-type enzyme, in absence of 3-phosphoglycerate | Solanum tuberosum |
4.87 | - |
purified mutant Mos(1-198) in absence of 3-phosphoglycerate | Zea mays |
4.87 | - |
purified mutant Mos(1-198) in absence of 3-phosphoglycerate | Solanum tuberosum |
6.14 | - |
purified mutant Mos(1-198, 430-475) in presence of 3-phosphoglycerate | Zea mays |
6.14 | - |
purified mutant Mos(1-198, 430-475) in presence of 3-phosphoglycerate | Solanum tuberosum |
6.81 | - |
purified mutant Mos(1-198, 377-429) in presence of 3-phosphoglycerate | Zea mays |
6.81 | - |
purified mutant Mos(1-198, 377-429) in presence of 3-phosphoglycerate | Solanum tuberosum |
7.29 | - |
purified mutant Mos(1-277) in presence of 3-phosphoglycerate | Zea mays |
7.29 | - |
purified mutant Mos(1-277) in presence of 3-phosphoglycerate | Solanum tuberosum |
8.59 | - |
purified mutant Mos(1-198, 377-475) in presence of 3-phosphoglycerate | Zea mays |
8.59 | - |
purified mutant Mos(1-198, 377-475) in presence of 3-phosphoglycerate | Solanum tuberosum |
9.38 | - |
purified mutant Mos(1-376) in presence of 3-phosphoglycerate | Zea mays |
9.38 | - |
purified mutant Mos(1-376) in presence of 3-phosphoglycerate | Solanum tuberosum |
10.87 | - |
purified mutant Mos(1-198) in presence of 3-phosphoglycerate | Zea mays |
10.87 | - |
purified mutant Mos(1-198) in presence of 3-phosphoglycerate | Solanum tuberosum |
21.81 | - |
purified wild-type enzyme in presence of 3-phosphoglycerate | Zea mays |
21.81 | - |
purified wild-type enzyme in presence of 3-phosphoglycerate | Solanum tuberosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | ADP-D-glucose + diphosphate | - |
r | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | ADP-D-glucose + diphosphate | - |
r | |
ATP + alpha-D-glucose 1-phosphate | substrate binding structure and kinetics, regulation of wild-type and mutant enzymes, mapping the polymorphic sites important in altered allosteric properties, overview | Zea mays | ADP-D-glucose + diphosphate | - |
r | |
ATP + alpha-D-glucose 1-phosphate | substrate binding structure and kinetics, regulation of wild-type and mutant enzymes, mapping the polymorphic sites important in altered allosteric properties, overview | Solanum tuberosum | ADP-D-glucose + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
adenosine diphosphate glucose pyrophosphorylase | - |
Zea mays |
adenosine diphosphate glucose pyrophosphorylase | - |
Solanum tuberosum |
ADP-glucose pyrophosphorylase | - |
Zea mays |
ADP-glucose pyrophosphorylase | - |
Solanum tuberosum |
AGPase | - |
Zea mays |
AGPase | - |
Solanum tuberosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Zea mays |
37 | - |
assay at | Solanum tuberosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
wild-type AGPAse in the absence of 3-phosphoglycerate has a half-life of 1 min, whereas mutant Mos(1-198) has a half-life of 5.5 min, when 3-phosphoglycerate is added to the wid-type enzyme, the half-life increases to 5.5 min and the half-life of the mutant is incrwased to 9.4 min | Zea mays |
42 | - |
wild-type AGPAse in the absence of 3-phosphoglycerate has a half-life of 1 min, whereas mutant Mos(1-198) has a half-life of 5.5 min, when 3-phosphoglycerate is added to the wid-type enzyme, the half-life increases to 5.5 min and the half-life of the mutant is incrwased to 9.4 min | Solanum tuberosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Zea mays |
7.4 | - |
assay at | Solanum tuberosum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
phosphate | the phosphate inhibition pattern of Mos(1-198) is complex and biphasic. Inhibition at relatively high phopshate concentrations is less than predicted at low phosphate concentrations, the calculated Ki for phosphate is about 1 mM before 50% inhibition and 36 mM after 50% inhibition. Inhibition kinetics of mutants in presence and absence of 3-phosphoglycerate | Solanum tuberosum | |
1 | - |
phosphate | the phosphate inhibition pattern of Mos(1-198) is complex and biphasic. Inhibition at relatively high phosphate concentrations is less than predicted at low phosphate concentrations, the calculated Ki for phosphate is about1 mM before 50% inhibition and 36 mM after 50% inhibition. Inhibition kinetics of mutants in presence and absence of 3-phosphoglycerate | Zea mays | |
1.4 | - |
phosphate | wild-type enzyme, in presence of 3-phosphoglycerate | Zea mays | |
1.4 | - |
phosphate | wild-type enzyme, in presence of 3-phosphoglycerate | Solanum tuberosum | |
8.7 | - |
phosphate | mutant Mos(1-198), in presence of 3-phosphoglycerate | Zea mays | |
8.7 | - |
phosphate | mutant Mos(1-198), in presence of 3-phosphoglycerate | Solanum tuberosum |