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G240V
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mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region
P227V
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mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region
P227V/G240V
-
mutant enzymes replaced with Val at the basal position of the flexible loop (P227V, G240V, and P227V/G240V) are identical with wild-type enzyme in their crystal structures, except the loop region
additional information
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deletion mutant of the loop and mutant with a nicked multifunctional loop. Cleavage of the loop results in a drastic increase in activity, which is similar to the results for the loop deletion. High concentrations of ATP inhibit the wild-type enzyme, while both nicked and loopless enzyme are not inhibited
additional information
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the mutant enzymes Arg86 and Asn283 are altered in their kinetic parameters, especially the Michaelis constant of gamma-Glu-Cys
additional information
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the crystal structure of the loopless mutant, in which the loop is replaced by 3 Gly residues, is identical with that of the wild-type enzyme. Replacement of the loop increases the Km-values, especially for glycine, and a 930fold decrease in turnover number. The loopless mutant shows gamma-Glu-L-Cys-dependent ATP hydrolase activity to almost the same extent as its glutathione synthetase activity
additional information
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construction of transgenic plants by overexpression of the enzyme in the cytosol of Brassica juncea, i.e. Indian mustard, transgenic plants show 3fold increased ability in the shoot compared to the wild-type to accumulate and tolerate heavy metals, e.g. cadmium, for use in detoxification
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Escherichia coli, Escherichia coli B / ATCC 11303
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32
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Escherichia coli, Escherichia coli B / ATCC 11303
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Flexibility impaired by mutations related the multifunctional roles of the loop in glutathione synthetase
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Escherichia coli
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33
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Escherichia coli, Escherichia coli B / ATCC 11303
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Tanaka, T.; Kato, H.; Nishioka, T.; Oda, J.
Mutational and proteolytic studies on a flexible loop in glutathione synthetase from Escherichia coli B: the loop and arginine 233 are critical for the catalytic reaction
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31
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Escherichia coli, Escherichia coli B / ATCC 11303
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A pseudo-Michaelis quarternary complex in the reverse reaction of a ligase: structure of Escherichia coli B glutathione synthetase complexed with ADP, glutathione, and sulfate at 2.0 A resolution
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35
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