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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
1-PMTR isomerase, 5'-methylthioribose-1-phosphate isomerase, 5-methylthio-5-deoxy-D-ribose-1-phosphate ketol-isomerase, 5-methylthioribose 1-phosphate isomerase, 5-methylthioribose-1-phosphate isomerase, Isomerase, methylthioribose 1-phosphate, M1Pi, methylthioribose-1-phosphate isomerase, Meu1p, MRDI,
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5'-methylthioribose-1-phosphate isomerase
5-methylthio-5-deoxy-D-ribose-1-phosphate ketol-isomerase
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5-methylthioribose 1-phosphate isomerase
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5-methylthioribose-1-phosphate isomerase
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Isomerase, methylthioribose 1-phosphate
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methylthioribose-1-phosphate isomerase
5'-methylthioribose-1-phosphate isomerase
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5'-methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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MRDI
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mediator of Rho-dependent invasion
MRI1
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mtnA
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MTR-1-P
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MTR-1-P isomerase
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PH0702
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S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate
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intramolecular oxidoreduction
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isomerization
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S-methyl-5-thio-alpha-D-ribose-1-phosphate aldose-ketose-isomerase
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
5-Methylthio-5-deoxy-D-ribose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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r
5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme of the pathway of the conversion of methylthioribose-1-phosphate to methionine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme of the pathway of the conversion of methylthioribose-1-phosphate to methionine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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additional information
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M1Pi does not require any metals to exhibit catalytic activity
additional information
M1Pi does not require any metals to exhibit catalytic activity
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RNAi
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stable knockdown of MRDI is carried out in A-375 cells
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RhoA
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MRDI is induced by RhoA and mediates RhoA-dependent cell invasion
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Breast Neoplasms
Proteomic analysis of infiltrating ductal carcinoma tissues by coupled 2-D DIGE/MS/MS analysis.
Neoplasms
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits.
Neoplasms
Proteomic analysis of infiltrating ductal carcinoma tissues by coupled 2-D DIGE/MS/MS analysis.
Trypanosomiasis
5'-Alkyl-substituted analogs of 5'-methylthioadenosine as trypanocides.
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brenda
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UniProt
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UniProt
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brenda
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brenda
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brenda
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brenda
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Uniprot
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UniProt
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brenda
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clone A has 5-methylthioribose-1-phosphate isomerase activity, clone D lacks this enzyme
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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physiological function
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MRDI is induced in metastatic cells by constitutive RhoA activation and promotes cell invasion
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Bacillus subtilis (strain 168)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
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dimer
x-ray crystallography
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cocrystallized with the substrate 5-methylthio-5-deoxy-D-ribose 1-phosphate, sitting drop vapour diffusion method, using 0.2 M K formate and 15% (w/v) polyethylene glycol 3350, at 20°C
sitting-drop vapour-diffusion method. Crystals diffract to 2.5 A at 100 K using synchrotron radiation and belong to the tetragonal space group P4(1), with unit-cell parameters a = b = 69.2 A, c = 154.7 A
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hanging drop vapor diffusion method, using 0.7 M sodium citrate and 100 mM imidazole, pH 8.0
in silico analysis of conserved residues and molecular modeling of structure. Residues of the enzymic activity of methylthioribose-1-phosphate isomerase that interact with the substrate are absolutely conserved. Residues essential for the hydrophobic stabilization of the substrate/product in the active site are conserved as well
sitting-drop vapour-diffusion method
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by nickel-nitrilotriacetic acid affinity chromatography, the His-tag is removed by treatment with tobacco etch virus protease
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expressed in Escherichia coli
into pCMV plasmid for expression in mammalian cells and into pMS356 plasmid for esxpression in Escherichia coli
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RhoA induces expression of MRDI
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brewing
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in sake brewed with Mri1 deletion strains, dimethyl trisulfide precursor is hardly detected, and dimethyl trisulfide production after sake storage is greatly reduced. The components of brewed sake are almost the same between the mutant and parent strains
brewing
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in sake brewed with Mri1 deletion strains, dimethyl trisulfide precursor is hardly detected, and dimethyl trisulfide production after sake storage is greatly reduced. The components of brewed sake are almost the same between the mutant and parent strains
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Trackman, P.C.; Abeles, R.H.
Methionine synthesis from 5'-S-methylthioaenosine. Resolution of enzyme activities and identification of 1-phospho-5-S-methylthioribulose
J. Biol. Chem.
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6717-6720
1983
Rattus norvegicus
brenda
Furfine, E.S.; Abeles, R.H.
Intermediates in the conversion of 5'-S-methylthioadenosine to methionine in Klebsiella pneumoniae
J. Biol. Chem.
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9598-9606
1988
Klebsiella pneumoniae
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Ghoda, L.Y.; Savarese, T.M.; Dexter, D.L.; Parks, R.E.; Trackman, P.C.; Abeles, R.H.
Characterization of a defect in the pathway for converting 5'-deoxy-5'-methylthioadenosine to methionine in a subline of a cultured heterogeneous human colon carcinoma
J. Biol. Chem.
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6715-6719
1984
Homo sapiens
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Tamura, H.; Matsumura, H.; Inoue, T.; Ashida, H.; Saito, Y.; Yokota, A.; Kai, Y.
Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis
Acta Crystallogr. Sect. F
F61
595-598
2005
Bacillus subtilis
brenda
Bumann, M.; Djafarzadeh, S.; Oberholzer, A.E.; Bigler, P.; Altmann, M.; Trachsel, H.; Baumann, U.
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits
J. Biol. Chem.
279
37087-37094
2004
Saccharomyces cerevisiae
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Pirkov, I.; Norbeck, J.; Gustafsson, L.; Albers, E.
A complete inventory of all enzymes in the eukaryotic methionine salvage pathway
FEBS J.
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4111-4120
2008
Saccharomyces cerevisiae
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Tamura, H.; Saito, Y.; Ashida, H.; Inoue, T.; Kai, Y.; Yokota, A.; Matsumura, H.
Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism
Protein Sci.
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126-135
2008
Bacillus subtilis, Bacillus subtilis (O31662)
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Kabuyama, Y.; Litman, E.S.; Templeton, P.D.; Metzner, S.I.; Witze, E.S.; Argast, G.M.; Langer, S.J.; Polvinen, K.; Shellman, Y.; Chan, D.; Shabb, J.B.; Fitzpatrick, J.E.; Resing, K.A.; Sousa, M.C.; Ahn, N.G.
A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme
Mol. Cell. Proteomics
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2308-2320
2009
Homo sapiens
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Templeton, P.D.; Litman, E.S.; Metzner, S.I.; Ahn, N.G.; Sousa, M.C.
Structure of mediator of RhoA-dependent invasion (MRDI) explains its dual function as a metabolic enzyme and a mediator of cell invasion
Biochemistry
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5675-5684
2013
Bacillus subtilis, Homo sapiens, Homo sapiens (Q9BV20)
brenda
Mary, C.; Duek, P.; Salleron, L.; Tienz, P.; Bumann, D.; Bairoch, A.; Lane, L.
Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway
PLoS ONE
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e52877
2012
Homo sapiens
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Gogoi, P.; Srivastava, A.; Jayaprakash, P.; Jeyakanthan, J.; Kanaujia, S.P.
In silico analysis suggests that PH0702 and PH0208 encode for methylthioribose-1-phosphate isomerase and ribose-1,5-bisphosphate isomerase, respectively, rather than aIF2Bbeta and aIF2Bdelta
Gene
575
118-126
2016
Pyrococcus horikoshii (O58433), Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428 (O58433)
brenda
Ikeda, Y.; Isogai, A.; Moriyoshi, Y.; Kanda, R.; Iwashita, K.; Fujii, T.
Construction of sake yeast with low production of dimethyl trisulfide precursor by a self-cloning method
J. Biosci. Bioeng.
125
419-424
2018
Saccharomyces cerevisiae, Saccharomyces cerevisiae K901
brenda
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5.3.1.23 S-methyl-5-thioribose-1-phosphate isomerase
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