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Information on EC 5.3.1.23 - S-methyl-5-thioribose-1-phosphate isomerase for references in articles please use BRENDA:EC5.3.1.23Word Map on EC 5.3.1.23
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
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S-methyl-5-thioribose-1-phosphate isomerase
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5-methylsulfanyl-alpha-D-ribose 1-phosphate = 5-methylsulfanyl-D-ribulose 1-phosphate
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intramolecular oxidoreduction
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isomerization
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S-methyl-5-thio-alpha-D-ribose 1-phosphate degradation I
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S-methyl-5-thio-alpha-D-ribose 1-phosphate degradation II
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methionine metabolism
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Cysteine and methionine metabolism
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S-methyl-5-thio-alpha-D-ribose-1-phosphate aldose-ketose-isomerase
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5'-methylthioribose-1-phosphate isomerase
5-methylthio-5-deoxy-D-ribose-1-phosphate ketol-isomerase
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5-methylthioribose 1-phosphate isomerase
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5-methylthioribose-1-phosphate isomerase
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Isomerase, methylthioribose 1-phosphate
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methylthioribose-1-phosphate isomerase
5'-methylthioribose-1-phosphate isomerase
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5'-methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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methylthioribose-1-phosphate isomerase
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MRDI
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mediator of Rho-dependent invasion
mtnA
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MTR-1-P
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MTR-1-P isomerase
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PH0702
gene name
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UniProt
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UniProt
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Uniprot
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physiological function
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MRDI is induced in metastatic cells by constitutive RhoA activation and promotes cell invasion
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
5-Methylthio-5-deoxy-D-ribose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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r
5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme of the pathway of the conversion of methylthioribose-1-phosphate to methionine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme of the pathway of the conversion of methylthioribose-1-phosphate to methionine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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5-Methylthio-5-deoxy-D-ribose 1-phosphate
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enzyme in synthesis of methionine from 5'-S-methylthioadenosine
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
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S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
Q9BV20
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additional information
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M1Pi does not require any metals to exhibit catalytic activity
additional information
M1Pi does not require any metals to exhibit catalytic activity
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RNAi
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stable knockdown of MRDI is carried out in A-375 cells
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RhoA
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MRDI is induced by RhoA and mediates RhoA-dependent cell invasion
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clone A has 5-methylthioribose-1-phosphate isomerase activity, clone D lacks this enzyme
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Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
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dimer
x-ray crystallography
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cocrystallized with the substrate 5-methylthio-5-deoxy-D-ribose 1-phosphate, sitting drop vapour diffusion method, using 0.2 M K formate and 15% (w/v) polyethylene glycol 3350, at 20°C
sitting-drop vapour-diffusion method. Crystals diffract to 2.5 A at 100 K using synchrotron radiation and belong to the tetragonal space group P4(1), with unit-cell parameters a = b = 69.2 A, c = 154.7 A
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hanging drop vapor diffusion method, using 0.7 M sodium citrate and 100 mM imidazole, pH 8.0
in silico analysis of conserved residues and molecular modeling of structure. Residues of the enzymic activity of methylthioribose-1-phosphate isomerase that interact with the substrate are absolutely conserved. Residues essential for the hydrophobic stabilization of the substrate/product in the active site are conserved as well
sitting-drop vapour-diffusion method
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by nickel-nitrilotriacetic acid affinity chromatography, the His-tag is removed by treatment with tobacco etch virus protease
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expressed in Escherichia coli
into pCMV plasmid for expression in mammalian cells and into pMS356 plasmid for esxpression in Escherichia coli
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RhoA induces expression of MRDI
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Breast Neoplasms
Proteomic analysis of infiltrating ductal carcinoma tissues by coupled 2-D DIGE/MS/MS analysis.
Neoplasms
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits.
Neoplasms
Proteomic analysis of infiltrating ductal carcinoma tissues by coupled 2-D DIGE/MS/MS analysis.
Trypanosomiasis
5'-Alkyl-substituted analogs of 5'-methylthioadenosine as trypanocides.
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Trackman, P.C.; Abeles, R.H.
Methionine synthesis from 5'-S-methylthioaenosine. Resolution of enzyme activities and identification of 1-phospho-5-S-methylthioribulose
J. Biol. Chem.
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6717-6720
1983
Rattus norvegicus
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Furfine, E.S.; Abeles, R.H.
Intermediates in the conversion of 5'-S-methylthioadenosine to methionine in Klebsiella pneumoniae
J. Biol. Chem.
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9598-9606
1988
Klebsiella pneumoniae
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Ghoda, L.Y.; Savarese, T.M.; Dexter, D.L.; Parks, R.E.; Trackman, P.C.; Abeles, R.H.
Characterization of a defect in the pathway for converting 5'-deoxy-5'-methylthioadenosine to methionine in a subline of a cultured heterogeneous human colon carcinoma
J. Biol. Chem.
259
6715-6719
1984
Homo sapiens
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Tamura, H.; Matsumura, H.; Inoue, T.; Ashida, H.; Saito, Y.; Yokota, A.; Kai, Y.
Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis
Acta Crystallogr. Sect. F
F61
595-598
2005
Bacillus subtilis
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Bumann, M.; Djafarzadeh, S.; Oberholzer, A.E.; Bigler, P.; Altmann, M.; Trachsel, H.; Baumann, U.
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits
J. Biol. Chem.
279
37087-37094
2004
Saccharomyces cerevisiae
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Pirkov, I.; Norbeck, J.; Gustafsson, L.; Albers, E.
A complete inventory of all enzymes in the eukaryotic methionine salvage pathway
FEBS J.
275
4111-4120
2008
Saccharomyces cerevisiae
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Tamura, H.; Saito, Y.; Ashida, H.; Inoue, T.; Kai, Y.; Yokota, A.; Matsumura, H.
Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism
Protein Sci.
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126-135
2008
Bacillus subtilis, Bacillus subtilis (O31662)
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Kabuyama, Y.; Litman, E.S.; Templeton, P.D.; Metzner, S.I.; Witze, E.S.; Argast, G.M.; Langer, S.J.; Polvinen, K.; Shellman, Y.; Chan, D.; Shabb, J.B.; Fitzpatrick, J.E.; Resing, K.A.; Sousa, M.C.; Ahn, N.G.
A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme
Mol. Cell. Proteomics
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2308-2320
2009
Homo sapiens
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Templeton, P.D.; Litman, E.S.; Metzner, S.I.; Ahn, N.G.; Sousa, M.C.
Structure of mediator of RhoA-dependent invasion (MRDI) explains its dual function as a metabolic enzyme and a mediator of cell invasion
Biochemistry
52
5675-5684
2013
Bacillus subtilis, Homo sapiens, Homo sapiens (Q9BV20)
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Mary, C.; Duek, P.; Salleron, L.; Tienz, P.; Bumann, D.; Bairoch, A.; Lane, L.
Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway
PLoS ONE
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e52877
2012
Homo sapiens
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Gogoi, P.; Srivastava, A.; Jayaprakash, P.; Jeyakanthan, J.; Kanaujia, S.P.
In silico analysis suggests that PH0702 and PH0208 encode for methylthioribose-1-phosphate isomerase and ribose-1,5-bisphosphate isomerase, respectively, rather than aIF2Bbeta and aIF2Bdelta
Gene
575
118-126
2016
Pyrococcus horikoshii (O58433), Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428 (O58433)
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