EC Number   |
|---|
    5.3.1.23 | cocrystallized with the substrate 5-methylthio-5-deoxy-D-ribose 1-phosphate, sitting drop vapour diffusion method, using 0.2 M K formate and 15% (w/v) polyethylene glycol 3350, at 20°C |
| 5.3.1.23 | hanging drop vapor diffusion method, using 0.7 M sodium citrate and 100 mM imidazole, pH 8.0 |
| 5.3.1.23 | in silico analysis of conserved residues and molecular modeling of structure. Residues of the enzymic activity of methylthioribose-1-phosphate isomerase that interact with the substrate are absolutely conserved. Residues essential for the hydrophobic stabilization of the substrate/product in the active site are conserved as well |
| 5.3.1.23 | purified enzyme in complex with product S-methyl-1-thio-D-ribulose 1-phosphate or a sulfate ion, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 50 mM Na HEPES, pH 7.4, and 1 mM EDTA, with or without 33 mM MTR-1-P, with 0.004-0.006 ml of reservoir solution containing 0.2 M K formate and 15% w/v PEG 3350, 20°C, X-ray diffraction structure determination and analysis at 2.4 A and 2.7 A resolution, respectively. The asymmetric unit contains two dimers for the MTRu-1-P-bound form, while one dimer is found in the asymmetric unit for the sulfate-bound form. Molecular replacement method using the crystal structure of Ypr118w (RCSB, PDB ID 1W2W) as a search model, and modeling |
| 5.3.1.23 | purified recombinant enzyme in two different forms of crystals belonging to space groups P3221 and P1, that are obtained at 4°C and 20°C, respectively. The crystal belonging to space group P3221 grows in a microbatch-under-oil set up by mixing 0.002 ml of protein solution and 0.002 ml of 0.1 M sodium citrate tribasic dihydrate, pH 5.6, and 2.5 M 1,6-hexanediol. Initial crystals belonging to space group P1 are obtained by hanging-drop vapor-diffusion method, mixing of 0.003 ml of protein solution with 0.001 ml of resevoir solution containing 0.05 M ammonium acetate, 0.1 M Tris, pH 8.0, 16% PEG 10000 and 0.05 M sodium acetate, method optimization, X-ray diffraction structure determination analysis at 2.30-2.65 A resolution, molecular replacement method using the three-dimensional atomic coordinates of M1Pi from Archaeoglobus fulgidus (PDB ID 1T5O) as the search model, modeling |
| 5.3.1.23 | sitting-drop vapour-diffusion method |
| 5.3.1.23 | sitting-drop vapour-diffusion method. Crystals diffract to 2.5 A at 100 K using synchrotron radiation and belong to the tetragonal space group P4(1), with unit-cell parameters a = b = 69.2 A, c = 154.7 A |
