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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 5.3.1.23Display the word mapDisplay the reaction diagram Show all sequences 5.3.1.23-
Show all pathways known for 5.3.1.23Display the word mapDisplay the reaction diagram Show all sequences 5.3.1.23by nickel-nitrilotriacetic acid affinity chromatography, the His-tag is removed by treatment with tobacco etch virus protease
Show all pathways known for 5.3.1.23Display the word mapDisplay the reaction diagram Show all sequences 5.3.1.23recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
Show all pathways known for 5.3.1.23Display the word mapDisplay the reaction diagram Show all sequences 5.3.1.23recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by gel filtration
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