Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 5.1.1.8 - 4-hydroxyproline epimerase and Organism(s) Brucella abortus and UniProt Accession Q2YLF3

for references in articles please use BRENDA:EC5.1.1.8
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     5 Isomerases
         5.1 Racemases and epimerases
             5.1.1 Acting on amino acids and derivatives
                5.1.1.8 4-hydroxyproline epimerase
IUBMB Comments
Also interconverts trans-4-hydroxy-D-proline and cis-4-hydroxy-L-proline.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Brucella abortus
UNIPROT: Q2YLF3
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Brucella abortus
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
hypre, 4-hydroxyproline 2-epimerase, hydroxyproline epimerase, hydroxyproline 2-epimerase, 4-hydroxyproline-2-epimerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-hydroxyproline 2-epimerase
-
Hydroxyproline epimerase
-
Epimerase, hydroxyproline
-
-
-
-
Hydroxyproline 2-epimerase
-
-
-
-
Hydroxyproline epimerase
-
-
-
-
L-Hydroxyproline epimerase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
epimerization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxyproline 2-epimerase
Also interconverts trans-4-hydroxy-D-proline and cis-4-hydroxy-L-proline.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-23-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans-4-hydroxy-L-proline
cis-4-hydroxy-D-proline
show the reaction diagram
-
-
-
r
trans-4-hydroxy-L-Pro
cis-4-hydroxy-D-Pro
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans-4-hydroxy-L-proline
cis-4-hydroxy-D-proline
show the reaction diagram
-
-
-
r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.8
trans-4-hydroxy-L-Pro
37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
4-hydroxyproline 2-epimerase is a member of proline racemase family
physiological function
the enzyme 4-hydroxyproline 2-epimerase reversibly catalyse the interconversion of trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline. The Brucella proline racemase (PrpA) or hydroxyproline epimerase (BaHyPRE) also exhibit B-cell mitogenic properties thereby causing B-lymphocytes polyclonal activation and increase secretion of interleukin (IL)-10. Importance of PrpA in brucellosis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinnat His-tagged Brucella abortus PrpA from Escherichia coli strain BL21 by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene prpA, live attenuated Salmonella typhimurium (ST) vector constitutively expressing heterologous Brucella abortus PrpA protein (ST-PrpA) is inoculated in BALB/c mice, recombinant expression of His-tagged Brucella abortus PrpA in Escherichia coli strain BL21
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
live attenuated Salmonella typhimurium (ST) vector constitutively expressing heterologous Brucella abortus S19 PrpA protein (ST-PrpA) is inoculated in BALB/c mice. No aggravation of morbidity is observed upon mice inoculation of ST-PrpA. Immunized mice show significantly quicker anti-Salmonella IgG responses as compared to ST only immunization, and an increase interleukin-4 production evident in in vitro pulsed mice splenocytes, splenocytes proliferative profiles and lymphocyte population evaluation, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity
molecular biology
PrpA can be used as a protein adjuvant in a live Salmonella delivery system, in order to increase humoral responses effectively without major interference on the cell mediated immunity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Goytia, M.; Chamond, N.; Cosson, A.; Coatnoan, N.; Hermant, D.; Berneman, A.; Minoprio, P.
Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens
PLoS ONE
2
e885
2007
Brucella abortus (Q57B94), Brucella abortus, Brucella melitensis (Q8YJ29), Brucella melitensis 16M (Q8YJ29), Brucella suis (Q8FYS0), Burkholderia pseudomallei (Q63NG7), Pseudomonas aeruginosa (Q9I476)
Manually annotated by BRENDA team
Lalsiamthara, J.; Lee, J.H.
Immuno-profiles of BALB/c mice inoculated with Salmonella vector delivering B-cell mitogen hydroxyproline epimerase
Mol. Immunol.
95
114-121
2018
Brucella abortus (Q2YLF3), Brucella abortus, Brucella abortus 2308 (Q2YLF3)
Manually annotated by BRENDA team