EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   5.1.1.8 | -999 |
- |
more |
Michaelis-Menten kinetics |
728328 |
| 5.1.1.8 | 0.0016 |
- |
trans-4-hydroxy-D-proline |
in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C |
714823 |
| 5.1.1.8 | 0.0024 |
- |
trans-4-hydroxy-L-proline |
in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C |
714823 |
| 5.1.1.8 | 0.502 |
- |
trans-3-hydroxy-L-proline |
pH 8.0, 50°C, wild-type enzyme |
730678 |
| 5.1.1.8 | 0.905 |
- |
trans-4-hydroxy-L-proline |
pH 8.0, 50°C, mutant enzyme F62V |
730678 |
| 5.1.1.8 | 2.17 |
- |
trans-4-hydroxy-L-proline |
pH 8.0, 50°C, mutant enzyme F420W |
730678 |
| 5.1.1.8 | 3.14 |
- |
cis-4-hydroxy-D-proline |
pH 8.0, 50°C, mutant enzyme F62S |
730678 |
| 5.1.1.8 | 4.18 |
- |
cis-4-hydroxy-D-proline |
pH 8.0, 50°C, mutant enzyme L221H |
730678 |
| 5.1.1.8 | 4.28 |
- |
trans-4-hydroxy-L-proline |
pH 8.0, 50°C, mutant enzyme W241F |
730678 |
| 5.1.1.8 | 6.08 |
- |
trans-4-hydroxy-L-proline |
pH 8.0, 50°C, wild-type enzyme |
730678 |
