Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 5 mM trans-3-hydroxy-L-proline, P5C, and L-proline | Pseudomonas aeruginosa | |
pyrrole 2-carboxylic acid | selective inhibitor, competitive inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
trans-4-hydroxy-D-proline | in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C | Pseudomonas aeruginosa | |
0.0024 | - |
trans-4-hydroxy-L-proline | in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cobalt resin column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-4-hydroxy-D-proline | - |
Pseudomonas aeruginosa | cis-4-hydroxy-L-proline | - |
r | |
trans-4-hydroxy-L-proline | - |
Pseudomonas aeruginosa | cis-4-hydroxy-D-proline | - |
r |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxyproline-2-epimerase | - |
Pseudomonas aeruginosa |
HyPRE | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is pyridoxal 5'-phosphate-independent | Pseudomonas aeruginosa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
2.3 | - |
in 50 mM HEPES, pH 8.0, with 2 mM beta-mercaptoethanol,1 mM EDTA, at 37°C | Pseudomonas aeruginosa | pyrrole 2-carboxylic acid |