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Information on EC 4.2.3.19 - ent-kaurene synthase and Organism(s) Stevia rebaudiana and UniProt Accession Q9XEH9

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.19 ent-kaurene synthase
IUBMB Comments
Part of a bifunctional enzyme involved in the biosynthesis of ent-kaurene. See also EC 5.5.1.13 (ent-copalyl diphosphate synthase)
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This record set is specific for:
Stevia rebaudiana
UNIPROT: Q9XEH9
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Word Map
The taxonomic range for the selected organisms is: Stevia rebaudiana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ent-kaurene synthase, ppcps/ks, kaurene synthase, cps/ks, ent-kaurene synthase b, class i ditps ent-kaurene synthase, ent-ks, ent-copalyl/ent-kaurene synthase, bifunctional cps/ks, ent-kaur-16-ene synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ent-kaurene synthase B
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ent-kaurene synthetase B
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclization
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diphosphate lysis
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SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate diphosphate-lyase (cyclizing, ent-kaurene-forming)
Part of a bifunctional enzyme involved in the biosynthesis of ent-kaurene. See also EC 5.5.1.13 (ent-copalyl diphosphate synthase)
CAS REGISTRY NUMBER
COMMENTARY hide
9055-64-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
ent-kaur-ene + diphosphate
show the reaction diagram
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-
-
?
ent-copalyl diphosphate
ent-kaurene + diphosphate
show the reaction diagram
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ent-copalyl diphosphate
ent-kaur-ene + diphosphate
show the reaction diagram
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-
-
?
ent-copalyl diphosphate
ent-kaurene + diphosphate
show the reaction diagram
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-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
geranylgeranyl diphosphate (GGPP) is an essential precursor in the biosynthesis of several diterpenes including ent-kaurene via formation of ent-copalyl diphosphate. Effect of isoprenoid precursors on ent-copalyl diphosphate and ent-kaurene production, ent-kaurene is quantified using GC-MS analysis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KS1_STERE
784
0
89402
Swiss-Prot
Chloroplast (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene KS1, a synthetic ent-kaurene module, expressing Stevia rebaudiana CPPS and KS, and Rhodobacter sphaeroides GGPPS (crtE gene), is constructed for ent-kaurene production in diverse Escherichia coli strains, best in strain MG1655
expressed in Escherichia coli BL21 (DE3) and MG1655 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kong, M.K.; Kang, H.J.; Kim, J.H.; Oh, S.H.; Lee, P.C.
Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli
J. Biotechnol.
214
95-102
2015
Stevia rebaudiana, Stevia rebaudiana (Q9XEH9)
Manually annotated by BRENDA team