EC Number |
Protein Variants |
Reference |
---|
4.2.3.19 | A676T |
site-directed mutagenesis of isozyme RcKSL2, mutation of the catalytically important residue results in 60fold increased product amount composed of essentially the same mix of ent-trachylobane and ent-kaurene as observed for the wild-type enzyme |
748876 |
4.2.3.19 | A710C |
no change in reaction product profile compared to wild-type |
714971 |
4.2.3.19 | A710F |
mutant converts geranylgeranyl diphosphate to ent-kaurene as the sole product |
714971 |
4.2.3.19 | A710G |
no change in reaction product profile compared to wild-type |
714971 |
4.2.3.19 | A710M |
mutant converted geranylgeranyl diphosphate to ent-kaurene as the sole product |
714971 |
4.2.3.19 | A710N |
no change in reaction product profile compared to wild-type |
714971 |
4.2.3.19 | A710S |
no change in reaction product profile compared to wild-type |
714971 |
4.2.3.19 | D132A |
low activity |
492252 |
4.2.3.19 | D320A |
low activity |
492252 |
4.2.3.19 | D635A/D636A |
mutation of the DDYFD motif in the kaurene synthase domain |
679843 |