Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 4.1.1.71 - 2-oxoglutarate decarboxylase and Organism(s) Mycolicibacterium smegmatis and UniProt Accession A0R2B1

for references in articles please use BRENDA:EC4.1.1.71
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.71 2-oxoglutarate decarboxylase
IUBMB Comments
Requires thiamine diphosphate. Highly specific.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mycolicibacterium smegmatis
UNIPROT: A0R2B1
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Mycolicibacterium smegmatis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
alkbh4, 2-oxoglutarate decarboxylase, alpha-ketoglutarate decarboxylase, fe(ii)/2og-dependent decarboxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-ketoglutarate decarboxylase
-
alpha-ketoglutarate decarboxylase
alpha-ketoglutaric decarboxylase
-
-
-
-
decarboxylase, oxoglutarate
-
-
-
-
KDC
-
-
-
-
oxoglutarate decarboxylase
-
-
-
-
pre-2-oxoglutarate decarboxylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
2-oxoglutarate carboxy-lyase (succinate-semialdehyde-forming)
Requires thiamine diphosphate. Highly specific.
CAS REGISTRY NUMBER
COMMENTARY hide
37205-42-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate
succinate semialdehyde + CO2
show the reaction diagram
-
-
-
?
2-oxoglutarate
succinate semialdehyde + CO2
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate
succinate semialdehyde + CO2
show the reaction diagram
-
-
-
?
2-oxoglutarate
succinate semialdehyde + CO2
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
x-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 39-46.5% (w/v) 2-methyl-2,4-pentanediol, 100 mM HEPES (pH 7.0), 200 mM NaCl
hanging drop vapor diffusion method, using 52-59% (w/v) 2-methyl-2,4-pentanediol and 22-25 mM sodium acetate
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Ni-NTA agarose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wagner, T.; Barilone, N.; Alzari, P.M.; Bellinzoni, M.
A dual conformation of the post-decarboxylation intermediate is associated with distinct enzyme states in mycobacterial KGD (alpha-ketoglutarate decarboxylase)
Biochem. J.
457
425-434
2014
Mycolicibacterium smegmatis (A0R3B1)
Manually annotated by BRENDA team
Wagner, T.; Bellinzoni, M.; Wehenkel, A.; OHare, H.M.; Alzari, P.M.
Functional plasticity and allosteric regulation of alpha-ketoglutarate decarboxylase in central mycobacterial metabolism
Chem. Biol.
18
1011-1020
2011
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycolicibacterium smegmatis (A0R2B1), Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 (A0R2B1)
Manually annotated by BRENDA team