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Literature summary for 4.1.1.71 extracted from

  • Wagner, T.; Bellinzoni, M.; Wehenkel, A.; OHare, H.M.; Alzari, P.M.
    Functional plasticity and allosteric regulation of alpha-ketoglutarate decarboxylase in central mycobacterial metabolism (2011), Chem. Biol., 18, 1011-1020.
    View publication on PubMed
Search for more literature for 4.1.1.71

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis
expressed in Escherichia coli BL21(DE3)pLysS cells Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 39-46.5% (w/v) 2-methyl-2,4-pentanediol, 100 mM HEPES (pH 7.0), 200 mM NaCl Mycobacterium tuberculosis
hanging drop vapor diffusion method, using 39-46.5% (w/v) 2-methyl-2,4-pentanediol, 100 mM HEPES (pH 7.0), 200 mM NaCl Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate Mycobacterium tuberculosis
-
 succinate semialdehyde + CO2
-
 ?
2-oxoglutarate Mycolicibacterium smegmatis
-
 succinate semialdehyde + CO2
-
 ?
2-oxoglutarate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 succinate semialdehyde + CO2
-
 ?
2-oxoglutarate Mycobacterium tuberculosis H37Rv
-
 succinate semialdehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Mycolicibacterium smegmatis A0R2B1
-
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0R2B1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Ni-NTA agarose column chromatography Mycobacterium tuberculosis
HisTrap column chromatography and Ni-NTA agarose column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate
-
 Mycobacterium tuberculosis succinate semialdehyde + CO2
-
 ?
2-oxoglutarate
-
 Mycolicibacterium smegmatis succinate semialdehyde + CO2
-
 ?
2-oxoglutarate
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 succinate semialdehyde + CO2
-
 ?
2-oxoglutarate
-
 Mycobacterium tuberculosis H37Rv succinate semialdehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Mycobacterium tuberculosis
homodimer x-ray crystallography Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
alpha-ketoglutarate decarboxylase
-
 Mycobacterium tuberculosis
alpha-ketoglutarate decarboxylase
-
 Mycolicibacterium smegmatis
Kgd
-
 Mycobacterium tuberculosis
Kgd
-
 Mycolicibacterium smegmatis