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Information on EC 3.6.5.2 - small monomeric GTPase

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EC Tree
IUBMB Comments
A family of about 50 enzymes with a molecular mass of 21 kDa that are distantly related to the alpha-subunit of heterotrimeric G-protein GTPase (EC 3.6.5.1). They are involved in cell-growth regulation (Ras subfamily), membrane vesicle traffic and uncoating (Rab and ARF subfamilies), nuclear protein import (Ran subfamily) and organization of the cytoskeleton (Rho and Rac subfamilies).
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This record set is specific for:
UNIPROT: P51153
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
k-ras, cdc42, h-ras, small gtpase, ef-tu, rho gtpase, rab11, rab3a, rab27a, rap1a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
small GTPase
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small GTPase Ras-related protein in brain 13
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GTP-phosphohydrolase
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GTPase
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guanine triphosphatase
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guanosine 5'-triphosphatase
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guanosine triphosphatase
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ribosomal GTPase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
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SYSTEMATIC NAME
IUBMB Comments
GTP phosphohydrolase (cell-regulating)
A family of about 50 enzymes with a molecular mass of 21 kDa that are distantly related to the alpha-subunit of heterotrimeric G-protein GTPase (EC 3.6.5.1). They are involved in cell-growth regulation (Ras subfamily), membrane vesicle traffic and uncoating (Rab and ARF subfamilies), nuclear protein import (Ran subfamily) and organization of the cytoskeleton (Rho and Rac subfamilies).
CAS REGISTRY NUMBER
COMMENTARY hide
9059-32-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + H2O
GDP + phosphate
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
knockdown of Rab13 blocks pterostilbene-induced mTOR inhibition and autophagy, whereas overexpression of the GTP-containing active form of Rab13 induces mTOR inhibition and autophagy in HUVECs. Upregulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells. Knockdown of Grb2 suppresses pterostilbene or upregulation of the active form of Rab13-induced autophagy. Knockdown of Rab13 inhibits the digestion of cytoplasmic components in pterostilbene-treated HUVECs numerous autophagosome- or autolysosome-like vesicular structures
metabolism
an increasing number of Rab GTPases have been shown to play either critical or accessory roles in various stages of autophagy. Rab1, Rab4, Rab5, Rab11, Rab12, Rab17, Rab23, Rab26, Rab30 and Rab32 participate in autophagosome formation. Rab13 is required for pterostilbene-induced autophagy
physiological function
Macroautophagy (i.e. autophagy) a highly conserved intracellular process by which cytosolic components and damaged organelles are enclosed and degraded by a double membrane-bound structure, can be viewed as a continuous and dynamic membrane transport and fusion process. The GTP-bound active form of Rab13 promotes the interaction between Rab13 and growth factor receptor-bound protein 2 (Grb2). It promotes macroautophagy in vascular endothelial cells. Rab13 activates the downstream AMP-activated protein kinase (AMPK) and blocks mammalian target of rapamycin (mTOR) signaling by its functional interaction with Grb2 to regulate autophagy in HUVECs. Rab13 is a regulator of autophagy in VECs under nutrient-enriched conditions. Upregulation of the active form of Rab13 increases its interaction with Grb2, which is required to activate AMPK, leading to the inhibition of mTOR and subsequently inducing autophagy. Rab13 regulated autophagy by interacting with Grb2 in HUVECs
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RAB13_HUMAN
203
0
22774
Swiss-Prot
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene RAB13, semiquantitative RT-PCR and quantitative real-time PCR expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Rab13 is the most significantly upregulated gene in pterostilbene-treated human umbilical VECs (HUVECs)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhang, L.; Dai, F.; Cui, L.; Zhou, B.; Guo, Y.
Up-regulation of the active form of small GTPase Rab13 promotes macroautophagy in vascular endothelial cells
Biochim. Biophys. Acta
1864
613-624
2017
Homo sapiens (P51153), Homo sapiens
Manually annotated by BRENDA team