Information on EC 3.5.3.8 - formimidoylglutamase

for references in articles please use BRENDA:EC3.5.3.8
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.3.8
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RECOMMENDED NAME
GeneOntology No.
formimidoylglutamase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-formimidoyl-L-glutamate + H2O = L-glutamate + formamide
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-N bond of an amidino group
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-histidine degradation I
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histidine metabolism
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Histidine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
N-formimidoyl-L-glutamate formimidoylhydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9054-92-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-formimidoyl-L-glutamate + H2O
formate + ammonium + glutamate
show the reaction diagram
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-
-
-
?
N-formimidoyl-L-glutamate + H2O
L-glutamate + formamide
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-formimidoyl-L-glutamate + H2O
formate + ammonium + glutamate
show the reaction diagram
-
-
-
-
?
N-formimidoyl-L-glutamate + H2O
L-glutamate + formamide
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
tetrahydrofolate
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-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetyl-L-glutamate
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competitive inhibitor
N-formyl-L-aspartate
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competitive inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9 - 530
N-formimidoyl-L-glutamate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 200
N-formimidoyl-L-glutamate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0052 - 5.2
N-formimidoyl-L-glutamate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
PDB
SCOP
CATH
UNIPROT
ORGANISM
P42068
Bacillus subtilis (strain 168);
Trypanosoma cruzi (strain CL Brener);
Q9KSQ2
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961);
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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gel filtration
220000
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ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 50000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 25% PEG (w/v) 3350, 0.1 M sodium acetate (pH 4.6)
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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90 min, 32% loss in activity
54
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15 min, 67% loss in activity
80
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15 min, totally inactivated
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 10 days, 75% in activity are lost
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification
Talon column chromatography and Q-HP anion exchange column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N114H
the mutant exhibits 35% loss of catalytic efficiency compared to the wild type enzyme
R144A
the mutant exhibits 99.3% loss of catalytic efficiency compared to the wild type enzyme
R144E
the mutant exhibits 99.9% loss of catalytic efficiency compared to the wild type enzyme
R144K
the mutant exhibits 77% loss of catalytic efficiency compared to the wild type enzyme
Show AA Sequence (3435 entries)
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