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Literature summary for 3.5.3.8 extracted from

  • Hai, Y.; Dugery, R.J.; Healy, D.; Christianson, D.W.
    Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme (2013), Biochemistry, 52, 9294-9309.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 25% PEG (w/v) 3350, 0.1 M sodium acetate (pH 4.6) Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
N114H the mutant exhibits 35% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
R144A the mutant exhibits 99.3% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
R144E the mutant exhibits 99.9% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
R144K the mutant exhibits 77% loss of catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9
-
 N-formimidoyl-L-glutamate mutant enzyme R144E, at pH 8.5 and 22°C Trypanosoma cruzi
20
-
 N-formimidoyl-L-glutamate mutant enzyme N114H, at pH 8.5 and 22°C Trypanosoma cruzi
40
-
 N-formimidoyl-L-glutamate wild type enzyme, at pH 8.5 and 22°C Trypanosoma cruzi
90
-
 N-formimidoyl-L-glutamate mutant enzyme R144A, at pH 8.5 and 22°C Trypanosoma cruzi
160
-
 N-formimidoyl-L-glutamate mutant enzyme R144K, at pH 8.5 and 22°C Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ contains two Mn2+ Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formimidoyl-L-glutamate + H2O Trypanosoma cruzi
-
 L-glutamate + formamide
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q4DSA0
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon column chromatography and Q-HP anion exchange column chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with L-arginine, agmatine, L-acetylarginine, alpha-guanidinoglutaric acid, L-homoarginine, L-2-amino-3-guanidinopropionic acid, 3-guanidinopropionic acid, and 4-guanidinobutyric acid Trypanosoma cruzi ?
-
 ?
N-formimidoyl-L-glutamate + H2O
-
 Trypanosoma cruzi L-glutamate + formamide
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
FIGase
-
 Trypanosoma cruzi
formiminoglutamase
-
 Trypanosoma cruzi
N-formimino-L-glutamate formiminohydrolase
-
 Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 N-formimidoyl-L-glutamate mutant enzyme R144E, at pH 8.5 and 22°C Trypanosoma cruzi
3.2
-
 N-formimidoyl-L-glutamate mutant enzyme R144A, at pH 8.5 and 22°C Trypanosoma cruzi
80
-
 N-formimidoyl-L-glutamate mutant enzyme N114H, at pH 8.5 and 22°C Trypanosoma cruzi
190
-
 N-formimidoyl-L-glutamate mutant enzyme R144K, at pH 8.5 and 22°C Trypanosoma cruzi
200
-
 N-formimidoyl-L-glutamate wild type enzyme, at pH 8.5 and 22°C Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0052
-
 N-formimidoyl-L-glutamate mutant enzyme R144E, at pH 8.5 and 22°C Trypanosoma cruzi
0.037
-
 N-formimidoyl-L-glutamate mutant enzyme R144A, at pH 8.5 and 22°C Trypanosoma cruzi
1.2
-
 N-formimidoyl-L-glutamate mutant enzyme R144K, at pH 8.5 and 22°C Trypanosoma cruzi
3.4
-
 N-formimidoyl-L-glutamate mutant enzyme N114H, at pH 8.5 and 22°C Trypanosoma cruzi
5.2
-
 N-formimidoyl-L-glutamate wild type enzyme, at pH 8.5 and 22°C Trypanosoma cruzi