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Literature summary for 3.5.3.8 extracted from
- Urocanase and N-formimino-L-glutamate formiminohydrolase of Bacillus subtilis, two enzymes of the histidine degradation pathway (1970), J. Biol. Chem., 245, 3536-3544.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 39 | - |
N-formimidoyl-L-glutamate | pH 7.4 | Bacillus subtilis |  |
| 530 | - |
N-formimidoyl-L-glutamate | pH 7.8 | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 220000 | - |
ultracentrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formimidoyl-L-glutamate + H2O | Bacillus subtilis | - |
L-glutamate + formamide | - |
? | |
| N-formimidoyl-L-glutamate + H2O | Bacillus subtilis SH-11 | - |
L-glutamate + formamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formimidoyl-L-glutamate + H2O | - |
Bacillus subtilis | L-glutamate + formamide | - |
? | |
| N-formimidoyl-L-glutamate + H2O | - |
Bacillus subtilis SH-11 | L-glutamate + formamide | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
90 min, 32% loss in activity | Bacillus subtilis |
| 54 | - |
15 min, 67% loss in activity | Bacillus subtilis |
| 80 | - |
15 min, totally inactivated | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Bacillus subtilis | |
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