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Literature summary for 3.5.3.8 extracted from

  • Fedorov, A.; Martํ-Arbona, R.; Nemmara, V.; Hitchcock, D.; Fedorov, E.; Almo, S.; Raushel, F.
    Structure of N-formimino-L-glutamate iminohydrolase from Pseudomonas aeruginosa (2015), Biochemistry, 54, 890-897 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.8

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method at room temperature, the crystal structure of the enzyme is determined in the presence of the inhibitors N-formimino-L-aspartate and N-guanidino-L-glutaric acid at resolutions of 1.9 and 1.4 A, respectively Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
N-formimino-L-aspartate
-
 Pseudomonas aeruginosa
N-guanidino-L-glutaric acid
-
 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the active site of Pa5106 contains a single zinc atom that is coordinated by His56, His58, His232, and Asp320. The nucleophilic solvent water molecule coordinates with the zinc atom at a distance of 2.0 A and is hydrogen bonded to Asp320 and His269 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formimidoyl-L-glutamate + H2O Pseudomonas aeruginosa
-
 L-glutamate + formamide
-
 ?
N-formimidoyl-L-glutamate + H2O Pseudomonas aeruginosa ATCC 15692
-
 L-glutamate + formamide
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HU77
-
-
Pseudomonas aeruginosa ATCC 15692 Q9HU77
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formimidoyl-L-glutamate + H2O
-
 Pseudomonas aeruginosa L-glutamate + formamide
-
 ?
N-formimidoyl-L-glutamate + H2O
-
 Pseudomonas aeruginosa ATCC 15692 L-glutamate + formamide
-
 ?

Synonyms

Synonyms Comment Organism
HutF
-
 Pseudomonas aeruginosa
N-formimino-L-glutamate iminohydrolase
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism the enzyme is involved in the histidine degradation pathway Pseudomonas aeruginosa