Information on EC 3.4.24.20 - peptidyl-Lys metalloendopeptidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
3.4.24.20
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RECOMMENDED NAME
GeneOntology No.
peptidyl-Lys metalloendopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage in proteins: -Xaa-/-Lys- (in which Xaa may be Pro)
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
65979-41-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
basidomycete, honey fungus
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl-ADKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-APKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ARKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKDSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKLSTSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKRSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-DADKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-DATKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O
?
show the reaction diagram
2,4-dinitrophenyl-STATKLSW + H2O
?
show the reaction diagram
-
-
?
Azoalbumin + H2O
?
show the reaction diagram
-
-
-
-
-
azocasein + H2O
?
show the reaction diagram
Azocoll + H2O
?
show the reaction diagram
Bovine insulin B-chain + H2O
?
show the reaction diagram
Bovine serum albumin + H2O
?
show the reaction diagram
Carboxymethylated aspartate aminotransferase + H2O
?
show the reaction diagram
-
from pig heart muscle, cleavage at 12 of 19 Lys and 3 of 26 Arg
-
-
-
Congo red elastin + H2O
?
show the reaction diagram
-
-
-
-
-
Equine heart cytochrome c + H2O
?
show the reaction diagram
Glucagon + H2O
?
show the reaction diagram
-
cleavage site: Ser11-Lys12, 2 products
-
-
-
Lysozyme + H2O
?
show the reaction diagram
-
-
-
-
-
Melittin + H2O
?
show the reaction diagram
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i.e. bee venom peptide, native or formylated, cleaves at Lys7, Lys21 and Lys23 residues or formyl-Lys residues
-
-
-
Oxidized ribonuclease A + H2O
?
show the reaction diagram
-
cleavage sites: -Xaa-Lys-, multiple products
-
-
-
Penicillopepsin + H2O
?
show the reaction diagram
Polyarginine + H2O
?
show the reaction diagram
Polylysine + H2O
?
show the reaction diagram
-
best substrate
-
-
-
protamine sulfate + H2O
?
show the reaction diagram
S-Carboxymethyl lysozyme + H2O
?
show the reaction diagram
-
cleavage sites: -Xaa-Lys-, multiple products
-
-
-
S-Carboxymethyl pepsinogen + H2O
?
show the reaction diagram
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cleavage sites: -Xaa-Lys-, 9 peptide products
-
-
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Vasopressin + H2O
?
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, zinc-depleted apoenzyme
Mn2+
-
activation, 0.1 mM, native enzyme and zinc-depleted apoenzyme
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2,2'-bipyridine
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try
-
2-mercaptoethanol
6-aminohexanoic acid
-
caseinolytic activity
Ag2+
-
1 mM, strong
agmatine
-
caseinolytic activity
antipain
Ba2+
-
1 mM, weak, caseinolytic activity
Ca2+
-
1 mM, weak, caseinolytic activity
Co2+
-
0.1 mM, caseinolytic activity
dithiothreitol
DL-homoarginine
-
caseinolytic activity
ethylamine
-
-
Guanidine-HCl
-
2 M and above, not at 1 M
imidazole
-
-
L-aminobutyric acid
-
caseinolytic activity
L-arginine
L-lysine
L-Lysine ethylester
-
caseinolytic activity
L-ornithine
methylamine
-
-
N-ethylmaleimide
n-Propylamine
-
-
p-chloromercuribenzoate
phosphoramidon
S-2-aminoethylcysteine
-
-
SDS
-
0.1% and above, not at 0.01%
Sodium citrate
-
0.1 M, not 0.01 M
tosyl-Lys chloromethyl ketone
tosyl-Phe chloromethyl ketone
-
weak, caseinolytic activity
Urea
-
5 M, not at 2 M
additional information
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0116
2,4-dinitrophenyl-ADKLSW
pH 9.5, 37°C
0.0111
2,4-dinitrophenyl-APKLSW
pH 9.5, 37°C
0.0152
2,4-dinitrophenyl-ARKLSW
pH 9.5, 37°C
0.0249
2,4-dinitrophenyl-ATKDSW
pH 9.5, 37°C
0.0133
2,4-dinitrophenyl-ATKLSTSW
pH 9.5, 37°C
0.0121
2,4-dinitrophenyl-ATKLSW
pH 9.5, 37°C
0.0177
2,4-dinitrophenyl-ATKRSW
pH 9.5, 37°C
0.0155
2,4-dinitrophenyl-DADKLSW
pH 9.5, 37°C
0.0088
2,4-dinitrophenyl-DATKLSW
pH 9.5, 37°C
0.0114
2,4-dinitrophenyl-STATKLSW
pH 9.5, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3
2,4-dinitrophenyl-ADKLSW
pH 9.5, 37°C
5.5
2,4-dinitrophenyl-APKLSW
pH 9.5, 37°C
54.7
2,4-dinitrophenyl-ARKLSW
pH 9.5, 37°C
0.25
2,4-dinitrophenyl-ATKDSW
pH 9.5, 37°C
0.99 - 17.6
2,4-dinitrophenyl-ATKLSTSW
3
2,4-dinitrophenyl-ATKLSW
pH 9.5, 37°C
3.7
2,4-dinitrophenyl-ATKRSW
pH 9.5, 37°C
0.12
2,4-dinitrophenyl-DADKLSW
pH 9.5, 37°C
5.1
2,4-dinitrophenyl-DATKLSW
pH 9.5, 37°C
21
2,4-dinitrophenyl-STATKLSW
pH 9.5, 37°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0399
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try
pH 9.5, 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
broad, azocoll as substrate
8.5
rather active with fluorogenic peptide substrates than at pH 5.0
8.5 - 9
-
azocoll as substrate
9
-
congo red elastin as substrate
additional information
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8 - 9.3
-
about half-maximal activity at pH 4.8 and 9.3, azocoll as substrate
5.5 - 10
-
about half-maximal activity at pH 5.5 and 10, azocoll as substrate
6 - 10.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
13500
-
1 * 13500, Armillaria mellea, SDS-PAGE
14000
-
Armillaria mellea, gel filtration
16600
-
Myxobacter sp. AL-1, minimal MW deduced from amino acid composition
16650
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Armillaria mellea, most probable MW deduced from amino acid composition
17000
-
Myxobacter sp. AL-1, sedimentation velocity experiments
18000
-
1 * 18000, Pleurotus ostreatus, SDS-PAGE
20000
-
x * 20000, Grifola frondosa, SDS-PAGE
additional information
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 20000, Grifola frondosa, SDS-PAGE
monomer
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
solved in 4 crystal forms, triclinic space group P1, unit cell parameters a : 30.8 A, b : 40.5 A, c : 30.5 A, monoclinic space group C2, unit cell parameters a : 43.6 A, b : 41.9 A, c : 76.9 A, tetragonal space group P4(3), unit cell parameters a : b : 30.2 A, c : 308.0 A, and hexangonal P6(5)22, unit cell parameters a : b 58.7 A, c : 145.2 A, hanging-drop vapour-diffusion method
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Myxobacter sp. AL-1
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 11
-
1 h, stable at 37°C, azocoll as substrate
31049
4 - 9
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18 h, 10% loss of caseinolytic activity at 4°C
31048
4 - 7
-
18 h stable at 37°C, azocoll as substrate
31049
5 - 10
-
18 h, about 20% loss of activity at 4°C
31047
7.2
-
3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C
31047
9
-
3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively
31047
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
1 h stable at pH 3-11, 18 h stable at pH 4-7
80
-
3 h, 40% loss of activity at pH 7.2, 30 min, about 90% loss of activity with Mn2+ instead of zinc
100
-
30 min, inactivation
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Stable to 5 h at 37°C in 0.1 M acetate buffer, pH 5, containing 2 M urea, 1 M guanidine-HCl or 0.01% SDS, inactivation at higher concentrations of urea, guanidine-HCl or SDS
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40°C, pH 7, at least a year
-
4°C, in various buffers, pH 5-10, about 20% loss of activity within 18 h
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
synthesis
-
isolation of blocked N-terminal peptides
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LINKS TO OTHER DATABASES (specific for EC-Number 3.4.24.20)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)