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Literature summary for 3.4.24.20 extracted from
- Myxobacter AL-1 protease II: specific peptide bond cleavage on the amino side of lysine (1972), J. Bacteriol., 112, 940-949.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Myxobacter sp. AL-1 | unidentified myxobacterium |
General Stability
| General Stability | Organism |
|---|---|
| No autolysis | unidentified myxobacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | not | unidentified myxobacterium |  |
| EDTA | - |
unidentified myxobacterium | |
| Hg2+ | 0.1 mM | unidentified myxobacterium | |
| Mg2+ | not | unidentified myxobacterium | |
| additional information | MnCl2 or soybean trypsin inhibitor; PMSF | unidentified myxobacterium | |
| N-ethylmaleimide | not | unidentified myxobacterium | |
| Sodium citrate | 0.1 M, not 0.01 M | unidentified myxobacterium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
unidentified myxobacterium | - |
- |
| soluble | - |
unidentified myxobacterium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | unidentified myxobacterium |
| 16600 | - |
Myxobacter sp. AL-1, minimal MW deduced from amino acid composition | unidentified myxobacterium |
| 17000 | - |
Myxobacter sp. AL-1, sedimentation velocity experiments | unidentified myxobacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| unidentified myxobacterium | - |
AL-1 | - |
| unidentified myxobacterium AL-1 | - |
AL-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
unidentified myxobacterium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
unidentified myxobacterium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
unidentified myxobacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Azoalbumin + H2O | - |
unidentified myxobacterium | ? | - |
? | |
| Azocoll + H2O | general protease substrate | unidentified myxobacterium | ? | - |
? | |
| Azocoll + H2O | general protease substrate | unidentified myxobacterium AL-1 | ? | - |
? | |
| Bovine insulin B-chain + H2O | cleaves only at Pro-Lys bond of oxidized insulin B-chain | unidentified myxobacterium | ? | - |
? | |
| Bovine serum albumin + H2O | - |
unidentified myxobacterium | ? | - |
? | |
| Bovine serum albumin + H2O | - |
unidentified myxobacterium AL-1 | ? | - |
? | |
| Equine heart cytochrome c + H2O | - |
unidentified myxobacterium | ? | - |
? | |
| Lysozyme + H2O | - |
unidentified myxobacterium | ? | - |
? | |
| additional information | similar specificity is shown by Myxobacter sp. AL-1 proteinase II | unidentified myxobacterium | ? | - |
? | |
| additional information | no cell-wall lytic activity | unidentified myxobacterium | ? | - |
? | |
| additional information | No substrates are Lys-Lys, Lys-Lys-Lys | unidentified myxobacterium | ? | - |
? | |
| additional information | similar specificity is shown by Myxobacter sp. AL-1 proteinase II | unidentified myxobacterium AL-1 | ? | - |
? | |
| additional information | no cell-wall lytic activity | unidentified myxobacterium AL-1 | ? | - |
? | |
| additional information | No substrates are Lys-Lys, Lys-Lys-Lys | unidentified myxobacterium AL-1 | ? | - |
? | |
| Polyarginine + H2O | - |
unidentified myxobacterium | ? | - |
? | |
| Vasopressin + H2O | i.e. Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Lys-Gly | unidentified myxobacterium | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | unidentified myxobacterium |
| 60 | - |
- |
unidentified myxobacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
1 h stable at pH 3-11, 18 h stable at pH 4-7 | unidentified myxobacterium |
| 50 | - |
18 h stable, about 10% loss of activity after 24 h | unidentified myxobacterium |
| 60 | - |
4 h, about 50% loss of activity | unidentified myxobacterium |
| 70 | - |
2 h, inactivation | unidentified myxobacterium |
| 70 | - |
30 min, about 50% loss of activity in Tris-HCl buffer, pH 7 | unidentified myxobacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | azocoll as substrate | unidentified myxobacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | about half-maximal activity at pH 5.5 and 10, azocoll as substrate | unidentified myxobacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | 1 h, stable at 37°C, azocoll as substrate | unidentified myxobacterium |
| 4 | 7 | 18 h stable at 37°C, azocoll as substrate | unidentified myxobacterium |
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