General Stability | Organism |
---|---|
Stable to 5 h at 37°C in 0.1 M acetate buffer, pH 5, containing 2 M urea, 1 M guanidine-HCl or 0.01% SDS, inactivation at higher concentrations of urea, guanidine-HCl or SDS | Pleurotus ostreatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pleurotus ostreatus | |
2,2'-bipyridine | 1 mM, weak | Pleurotus ostreatus | |
2-mercaptoethanol | not | Pleurotus ostreatus | |
Ag2+ | 1 mM, strong | Pleurotus ostreatus | |
antipain | not | Pleurotus ostreatus | |
Cu2+ | 1 mM, caseinolytic activity; strong | Pleurotus ostreatus | |
dithiothreitol | caseinolytic activity; weak | Pleurotus ostreatus | |
EDTA | 50 mM, weak at 1 mM; fully restorable by Zn2+, Mn2+, Co2+ | Pleurotus ostreatus | |
Guanidine-HCl | 2 M and above, not at 1 M | Pleurotus ostreatus | |
Hg2+ | 1 mM; strong, caseinolytic activity | Pleurotus ostreatus | |
additional information | L-cysteine; no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF | Pleurotus ostreatus | |
N-ethylmaleimide | weak, caseinolytic activity | Pleurotus ostreatus | |
p-chloromercuribenzoate | weak, caseinolytic activity | Pleurotus ostreatus | |
phosphoramidon | not | Pleurotus ostreatus | |
SDS | 0.1% and above, not at 0.01% | Pleurotus ostreatus | |
tosyl-Lys chloromethyl ketone | weak, caseinolytic | Pleurotus ostreatus | |
Urea | 5 M, not at 2 M | Pleurotus ostreatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pleurotus ostreatus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | requirement, 1 atom per molecule of native enzyme | Pleurotus ostreatus | |
Zinc | atomic absorption spectroscopy | Pleurotus ostreatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
1 * 18000, Pleurotus ostreatus, SDS-PAGE | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pleurotus ostreatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Pleurotus ostreatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
Bovine insulin B-chain + H2O | cleavage site: Pro29-Lys30, yielding 2 fragments: dipeptide Lys-Ala and substrate devoid of 2 C-terminal amino acids | Pleurotus ostreatus | ? | - |
? | |
Equine heart cytochrome c + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
Polyarginine + H2O | - |
Pleurotus ostreatus | ? | - |
? | |
Polylysine + H2O | best substrate | Pleurotus ostreatus | ? | - |
? | |
protamine sulfate + H2O | - |
Pleurotus ostreatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 18000, Pleurotus ostreatus, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pleurotus ostreatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, 10% loss of caseinolytic activity | Pleurotus ostreatus |
70 | - |
15 min, inactivation | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 8.35 | Pleurotus ostreatus |
5.6 | - |
azocoll or azocasein as substrate | Pleurotus ostreatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | 18 h, 10% loss of caseinolytic activity at 4°C | Pleurotus ostreatus |