BRENDA - Enzyme Database
show all sequences of 3.4.24.20

Characterization of a thermostable lysine-specific metalloendopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa

Nonaka, T.; Ishikawa, H.; Tsumuraya, Y.; Hashimoto, Y.; Dohmae, N.; Takio, K.; J. Biochem. 118, 1014-1020 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
strong, caseinolytic activity
Grifola frondosa
2,2'-bipyridine
strong, caseinolytic activity
Grifola frondosa
2-mercaptoethanol
weak, caseinolytic activity
Grifola frondosa
6-aminohexanoic acid
caseinolytic activity
Grifola frondosa
agmatine
caseinolytic activity
Grifola frondosa
antipain
weak, caseinolytic activity
Grifola frondosa
Ba2+
1 mM, weak, caseinolytic activity
Grifola frondosa
Ca2+
1 mM, weak, caseinolytic activity
Grifola frondosa
Co2+
0.1 mM, caseinolytic activity
Grifola frondosa
Cu2+
1 mM, caseinolytic activity
Grifola frondosa
dithiothreitol
caseinolytic activity; strong
Grifola frondosa
DL-homoarginine
caseinolytic activity
Grifola frondosa
EDTA
fully restorable by Ca2+; fully restorable by Zn2+, Mn2+, Co2+; strong, caseinolytic activity
Grifola frondosa
Hg2+
strong, caseinolytic activity
Grifola frondosa
L-aminobutyric acid
caseinolytic activity
Grifola frondosa
L-arginine
caseinolytic activity
Grifola frondosa
L-lysine
caseinolytic activity
Grifola frondosa
L-Lysine ethylester
caseinolytic activity
Grifola frondosa
L-ornithine
caseinolytic activity
Grifola frondosa
Mg2+
1 mM, weak, caseinolytic activity
Grifola frondosa
additional information
no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF; zinc acetate, elastatinal, Lys-Lys, 4 M urea
Grifola frondosa
p-chloromercuribenzoate
not
Grifola frondosa
phosphoramidon
weak, caseinolytic activity
Grifola frondosa
tosyl-Lys chloromethyl ketone
not
Grifola frondosa
tosyl-Phe chloromethyl ketone
weak, caseinolytic activity
Grifola frondosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Grifola frondosa
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, zinc-depleted apoenzyme
Grifola frondosa
Co2+
activation, zinc-depleted apoenzyme
Grifola frondosa
Mn2+
activation, 0.1 mM, native enzyme and zinc-depleted apoenzyme
Grifola frondosa
Zinc
atomic absorption spectroscopy; requirement, 1 atom per molecule of native enzyme; Zn2+ activates zinc-depleted apoenzyme
Grifola frondosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, Grifola frondosa, SDS-PAGE
Grifola frondosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Grifola frondosa
-
i.e. Maitake, var. S.F.Gray
-
Purification (Commentary)
Commentary
Organism
-
Grifola frondosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit body
-
Grifola frondosa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Grifola frondosa
Storage Stability
Storage Stability
Organism
-40°C, pH 7, at least a year
Grifola frondosa
4°C, in various buffers, pH 5-10, about 20% loss of activity within 18 h
Grifola frondosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
azocasein + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Azocoll + H2O
general protease substrate
31047
Grifola frondosa
?
-
-
-
-
Bovine serum albumin + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Congo red elastin + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Equine heart cytochrome c + H2O
cleaves-X-Lys bonds
31047
Grifola frondosa
?
-
-
-
-
additional information
specific cleavage of acyl-Lys bonds (-X-Lys-) in polypeptides
31047
Grifola frondosa
?
-
-
-
-
protamine sulfate + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 20000, Grifola frondosa, SDS-PAGE
Grifola frondosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Grifola frondosa
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
3 h, about 30% loss of activity at pH 9
Grifola frondosa
60
-
3 h, about 55% loss of activity at pH 9
Grifola frondosa
70
-
3 h, about 40% loss of activity with Mn2+ instead of zinc; 3 h, about 90% loss of activity at pH 9; and below, 3 h stable at pH 7.2
Grifola frondosa
80
-
3 h, 40% loss of activity at pH 7.2, 30 min, about 90% loss of activity with Mn2+ instead of zinc
Grifola frondosa
100
-
30 min, inactivation
Grifola frondosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 7.46
Grifola frondosa
9
-
congo red elastin as substrate
Grifola frondosa
9.5
-
azocoll or azocasein as substrate
Grifola frondosa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
more than 50% of maximal activity at pH 6 and 10.5
Grifola frondosa
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
18 h, about 20% loss of activity at 4°C
Grifola frondosa
7.2
-
3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C
Grifola frondosa
9
-
3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively
Grifola frondosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
strong, caseinolytic activity
Grifola frondosa
2,2'-bipyridine
strong, caseinolytic activity
Grifola frondosa
2-mercaptoethanol
weak, caseinolytic activity
Grifola frondosa
6-aminohexanoic acid
caseinolytic activity
Grifola frondosa
agmatine
caseinolytic activity
Grifola frondosa
antipain
weak, caseinolytic activity
Grifola frondosa
Ba2+
1 mM, weak, caseinolytic activity
Grifola frondosa
Ca2+
1 mM, weak, caseinolytic activity
Grifola frondosa
Co2+
0.1 mM, caseinolytic activity
Grifola frondosa
Cu2+
1 mM, caseinolytic activity
Grifola frondosa
dithiothreitol
caseinolytic activity; strong
Grifola frondosa
DL-homoarginine
caseinolytic activity
Grifola frondosa
EDTA
fully restorable by Ca2+; fully restorable by Zn2+, Mn2+, Co2+; strong, caseinolytic activity
Grifola frondosa
Hg2+
strong, caseinolytic activity
Grifola frondosa
L-aminobutyric acid
caseinolytic activity
Grifola frondosa
L-arginine
caseinolytic activity
Grifola frondosa
L-lysine
caseinolytic activity
Grifola frondosa
L-Lysine ethylester
caseinolytic activity
Grifola frondosa
L-ornithine
caseinolytic activity
Grifola frondosa
Mg2+
1 mM, weak, caseinolytic activity
Grifola frondosa
additional information
no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF; zinc acetate, elastatinal, Lys-Lys, 4 M urea
Grifola frondosa
p-chloromercuribenzoate
not
Grifola frondosa
phosphoramidon
weak, caseinolytic activity
Grifola frondosa
tosyl-Lys chloromethyl ketone
not
Grifola frondosa
tosyl-Phe chloromethyl ketone
weak, caseinolytic activity
Grifola frondosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Grifola frondosa
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, zinc-depleted apoenzyme
Grifola frondosa
Co2+
activation, zinc-depleted apoenzyme
Grifola frondosa
Mn2+
activation, 0.1 mM, native enzyme and zinc-depleted apoenzyme
Grifola frondosa
Zinc
atomic absorption spectroscopy; requirement, 1 atom per molecule of native enzyme; Zn2+ activates zinc-depleted apoenzyme
Grifola frondosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
x * 20000, Grifola frondosa, SDS-PAGE
Grifola frondosa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Grifola frondosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit body
-
Grifola frondosa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Grifola frondosa
Storage Stability (protein specific)
Storage Stability
Organism
-40°C, pH 7, at least a year
Grifola frondosa
4°C, in various buffers, pH 5-10, about 20% loss of activity within 18 h
Grifola frondosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
azocasein + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Azocoll + H2O
general protease substrate
31047
Grifola frondosa
?
-
-
-
-
Bovine serum albumin + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Congo red elastin + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Equine heart cytochrome c + H2O
cleaves-X-Lys bonds
31047
Grifola frondosa
?
-
-
-
-
additional information
specific cleavage of acyl-Lys bonds (-X-Lys-) in polypeptides
31047
Grifola frondosa
?
-
-
-
-
protamine sulfate + H2O
-
31047
Grifola frondosa
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 20000, Grifola frondosa, SDS-PAGE
Grifola frondosa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Grifola frondosa
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
3 h, about 30% loss of activity at pH 9
Grifola frondosa
60
-
3 h, about 55% loss of activity at pH 9
Grifola frondosa
70
-
3 h, about 40% loss of activity with Mn2+ instead of zinc; 3 h, about 90% loss of activity at pH 9; and below, 3 h stable at pH 7.2
Grifola frondosa
80
-
3 h, 40% loss of activity at pH 7.2, 30 min, about 90% loss of activity with Mn2+ instead of zinc
Grifola frondosa
100
-
30 min, inactivation
Grifola frondosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 7.46
Grifola frondosa
9
-
congo red elastin as substrate
Grifola frondosa
9.5
-
azocoll or azocasein as substrate
Grifola frondosa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
more than 50% of maximal activity at pH 6 and 10.5
Grifola frondosa
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
18 h, about 20% loss of activity at 4°C
Grifola frondosa
7.2
-
3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C
Grifola frondosa
9
-
3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively
Grifola frondosa
Other publictions for EC 3.4.24.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718383
Kishimoto
Accurate mass comparison coupl ...
Grifola frondosa
Proteomics
11
485-489
2011
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1
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1
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1
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-
-
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684398
Coussot
A method for the isolation of ...
Bos taurus
Anal. Biochem.
361
302-304
2007
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1
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1
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1
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670021
Rao
Proteolytic 18O labeling by pe ...
Grifola frondosa
J. Proteome Res.
4
507-514
2005
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1
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1
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670269
Rao
Peptidyl-Lys metalloendopeptid ...
Grifola frondosa
Mol. Cell. Proteomics
4
1550-1557
2005
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1
-
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-
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-
-
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2
-
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1
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649255
Hori
Structure of a new 'aspzincin' ...
Grifola frondosa
Acta Crystallogr. Sect. D
D57
361-368
2001
-
-
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1
-
-
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1
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2
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1
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1
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1
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651750
Nonaka
Kinetic characterization of ly ...
Grifola frondosa, Pleurotus ostreatus
J. Biochem.
124
157-162
1998
-
-
-
-
-
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1
10
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3
-
-
-
4
-
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2
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16
-
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11
3
1
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1
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1
1
10
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3
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2
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16
-
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11
3
1
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31047
Nonaka
Characterization of a thermost ...
Grifola frondosa
J. Biochem.
118
1014-1020
1995
-
-
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25
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1
4
1
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1
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1
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1
1
2
7
1
1
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5
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3
1
3
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25
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1
4
1
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1
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1
1
2
7
1
1
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5
-
3
1
3
-
-
-
-
-
-
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31048
Dohmae
Purification and characterizat ...
Pleurotus ostreatus
Biosci. Biotechnol. Biochem.
59
2074-2080
1995
-
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1
17
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1
1
1
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3
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1
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1
1
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6
1
1
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2
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2
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1
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1
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17
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1
1
1
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1
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1
1
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6
1
1
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2
-
2
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1
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-
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-
31046
Barry
Cleavage by trypsin and by the ...
Armillaria mellea
Biochem. J.
193
737-742
1981
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1
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1
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31045
Lewis
Specificity and inhibition stu ...
Armillaria mellea
Biochim. Biophys. Acta
522
551-560
1978
-
-
-
-
-
-
10
-
-
1
4
-
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1
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1
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1
1
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8
1
1
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1
1
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-
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-
-
-
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10
-
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1
4
-
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1
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1
1
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8
1
1
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1
1
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-
-
-
-
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31050
Cunningham
Amino acid sequence of penicil ...
unidentified myxobacterium, unidentified myxobacterium AL-1
Can. J. Biochem.
54
902-914
1976
-
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4
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6
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6
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31044
Doonan
The primary structure of aspar ...
Armillaria mellea
Biochem. J.
149
497-506
1975
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1
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31049
Wingard
Myxobacter AL-1 protease II: s ...
unidentified myxobacterium, unidentified myxobacterium AL-1
J. Bacteriol.
112
940-949
1972
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