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Information on EC 3.4.21.B39 - stratum corneum tryptic enzyme and Organism(s) Mus musculus and UniProt Accession P15945

for references in articles please use BRENDA:EC3.4.21.B39
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B39 stratum corneum tryptic enzyme
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This record set is specific for:
Mus musculus
UNIPROT: P15945
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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proteolytic cleavage of polypeptides
Synonyms
kallikrein 5, stratum corneum tryptic enzyme, kallikrein-related peptidase 5, human kallikrein 5, kallikrein-5, klk-l2, kallikrein protein 5, tissue kallikrein 5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tissue kallikrein
-
kallikrein 5
S01.017
-
-
-
-
tissue kallikrein 5
-
serine protease with trypsin-like activity for Arg at the P1 position
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
272788-46-2
-
9001-01-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
collagen type IV + H2O
?
show the reaction diagram
-
-
-
-
?
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O
diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
high-molecular-weight kininogen + H2O
?
show the reaction diagram
-
-
-
-
?
insulin-like growth factor-binding protein-3 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
antipain
-
13% inhibition at 0.2 mM
Aprotinin
-
34% inhibition at 0.1 g/ml
diisopropylfluorophosphate
-
87% inhibition at 2 mM
E-64
-
12% inhibition at 0.5 mM
leupeptin
-
70% inhibition at 0.1 mM
N-alpha-p-tosyl-L-leucine-chloromethylketone
-
14% inhibition at 0.2 mM
N-alpha-p-tosyl-L-phenylalanine-chloromethylketone
-
14% inhibition at 0.2 mM
o-phenanthroline
-
10% inhibition at 2 mM
additional information
-
not inhibited by soybean trypsin inhibitor, benzamidine, pepstatin, EDTA, and chymostatin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.13
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.11
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.03
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.03
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.13
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.11
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.03
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
0.03
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin
-
in 0.1 M Tris-HCl buffer (pH 8.0)
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the role of skin pH in the pathogenesis of atopic dermatitis is explored using the NC/Tnd murine atopic dermatitis model. Alterations in skin pH directly modulate kallikrein 5 activity leading to skin barrier dysfunction, itch, and dermatitis via the protease-activated receptor 2 thymic stromal lymphopoietin pathway
physiological function
the enzyme plays a role in skin desquamation. KLK5 activates proteinase-activated receptor, which is involved in itching
malfunction
kallikrein 5 induces atopic dermatitis-like lesions through PAR2-mediated thymic stromal lymphopoietin expression in Netherton syndrome, phenotype, overview
metabolism
KLK5 is part of an original biological cascade in which unregulated KLK5 directly activates proteinase-activated receptor 2 and induces nuclear factor kappaB-mediated overexpression of TSLP, intercellular adhesion molecule 1, tumor necrosis factor alpha, and IL8. This proinflammatory and proallergic pathway is independent of the primary epithelial failure and is activated under basal conditions in NS keratinocytes
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
K1KB5_MOUSE
261
0
28748
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25500
-
non-reducing SDS-PAGE
28500
-
reducing SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 2 months, no loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-chelate column chromatography and Resource Q column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression of KLK5 is increased in the epidermis of patients with atopic dermatitis and its activity increases in atopic dermatitis-like lesions
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q9Y337), Mus musculus (P15945)
Manually annotated by BRENDA team
Rajapakse, S.; Ogiwara, K.; Yamano, N.; Kimura, A.; Hirata, K.; Takahashi, S.; Takahashi, T.
Characterization of mouse tissue kallikrein 5
Zool. Sci.
23
963-968
2006
Mus musculus
Manually annotated by BRENDA team
Briot, A.; Deraison, C.; Lacroix, M.; Bonnart, C.; Robin, A.; Besson, C.; Dubus, P.; Hovnanian, A.
Kallikrein 5 induces atopic dermatitis-like lesions through PAR2-mediated thymic stromal lymphopoietin expression in Netherton syndrome
J. Exp. Med.
206
1135-1147
2009
Mus musculus (Q9D140), Mus musculus
Manually annotated by BRENDA team
Andoh, T.; Tsujii, K.; Kuraishi, Y.
Increase in pruritogenic kallikrein 5 in the skin of NC mice with chronic dermatitis
Exp. Dermatol.
24
978-980
2015
Mus musculus (P15945)
Manually annotated by BRENDA team
Jang, H.; Matsuda, A.; Jung, K.; Karasawa, K.; Matsuda, K.; Oida, K.; Ishizaka, S.; Ahn, G.; Amagai, Y.; Moon, C.; Kim, S.H.; Arkwright, P.D.; Takamori, K.; Matsuda, H.; Tanaka, A.
Skin pH is the master switch of kallikrein 5-mediated skin barrier destruction in a murine atopic dermatitis model
J. Invest. Dermatol.
136
127-135
2016
Mus musculus (P15945), Mus musculus
Manually annotated by BRENDA team