Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | 13% inhibition at 0.2 mM | Mus musculus | |
Aprotinin | 34% inhibition at 0.1 g/ml | Mus musculus | |
diisopropylfluorophosphate | 87% inhibition at 2 mM | Mus musculus | |
E-64 | 12% inhibition at 0.5 mM | Mus musculus | |
leupeptin | 70% inhibition at 0.1 mM | Mus musculus | |
additional information | not inhibited by soybean trypsin inhibitor, benzamidine, pepstatin, EDTA, and chymostatin | Mus musculus | |
N-alpha-p-tosyl-L-leucine-chloromethylketone | 14% inhibition at 0.2 mM | Mus musculus | |
N-alpha-p-tosyl-L-phenylalanine-chloromethylketone | 14% inhibition at 0.2 mM | Mus musculus | |
o-phenanthroline | 10% inhibition at 2 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.03 | - |
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.05 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.11 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.13 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
non-reducing SDS-PAGE | Mus musculus |
28500 | - |
reducing SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelate column chromatography and Resource Q column chromatography | Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, 2 months, no loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
collagen type IV + H2O | - |
Mus musculus | ? | - |
? | |
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Fibronectin + H2O | - |
Mus musculus | ? | - |
? | |
Gelatin + H2O | - |
Mus musculus | ? | - |
? | |
high-molecular-weight kininogen + H2O | - |
Mus musculus | ? | - |
? | |
insulin-like growth factor-binding protein-3 + H2O | - |
Mus musculus | ? | - |
? | |
additional information | no activity with tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin, succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, casein, laminin, and single-chain tissue-type plasminogen activator | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tissue kallikrein 5 | serine protease with trypsin-like activity for Arg at the P1 position | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.03 | - |
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.05 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.11 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
0.13 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Mus musculus |