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(R)-2-aminobutanamide + H2O
(R)-2-aminobutanoic acid + NH3
D-2-aminobutanamide + H2O
D-aminobutanoic acid + NH3
-
racemic, preferred substrate
-
-
?
D-2-aminobutyric acid amide + H2O
D-aminobutyric acid + NH3
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
D-Ala-3-aminopentane amide + H2O
?
-
-
-
-
?
D-Ala-4-nitroanilide + H2O
D-Ala + 4-nitroaniline
D-Ala-D-Ala + H2O
2 D-Ala
-
-
-
?
D-Ala-Gly + H2O
D-Ala + Gly
D-Ala-Gly-Gly + H2O
D-Ala + Gly-Gly
D-Ala-L-Ala + H2O
D-Ala + L-Ala
-
-
-
?
D-Ala-L-Ala-L-Ala + H2O
D-Ala + Ala-Ala
-
-
-
-
?
D-alanine amide + H2O
D-Ala + NH3
D-alanine amide + H2O
D-alanine + NH3
-
28.3% of the activity with D-2-aminobutanamide
-
-
?
D-alanine benzylamide + H2O
D-Ala + benzylamine
-
-
-
-
?
D-alanine n-butylamide + H2O
D-Ala + n-butylamine
-
-
-
-
?
D-serine amide + H2O
D-serine + NH3
D-threonine amide + H2O
D-Thr + NH3
-
-
-
-
?
fluoresceyl-Gly-6-aminopenicillanic acid + H2O
?
-
penicillin-binding activity of mutant lacking residues 476-486
-
-
?
Gly 4-nitroanilide + H2O
Gly + 4-nitroaniline
Gly-Gly + H2O
Gly + Gly
-
-
-
?
Gly-Gly-Gly + 2 H2O
3 Gly
-
-
-
?
Gly-L-Ala + H2O
Gly + L-Ala
Gly-L-Cys + H2O
Gly + L-Cys
Gly-L-Cys-Gly + H2O
Gly + L-Cys-Gly
-
-
-
?
glycine amide + H2O
Gly + NH3
-
-
-
-
?
glycine amide + H2O
glycine + NH3
-
29.6% of the activity with D-2-aminobutanamide
-
-
?
L-2-aminobutyric amide + H2O
D-2-aminobutyric acid + NH3
-
in the presence of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae
-
-
?
L-Ala 4-nitroanilide + H2O
L-Ala + 4-nitroaniline
L-Ala-D-Ala + H2O
L-Ala + D-Ala
-
-
-
?
L-alanine amide + H2O
D-alanine + NH3
-
in the presence of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae
-
-
?
L-methionine amide + H2O
D-methionine + NH3
-
in the presence of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae
-
-
?
L-serine amide + H2O
D-serine + NH3
-
in the presence of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae
-
-
?
Nalpha, Nalpha-Ac2-L-Lys-D-Ala-D-Ala + H2O
Nalpha, Nalpha-Ac2-L-Lys-D-Ala + D-Ala
-
wild-type, no substrate. Mutant lacking residues 476-486 with or without mutation N275R, small D,D-carboxypeptidase activity
-
-
?
Nalpha-Ac-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Ac-L-Lys-D-Ala + D-Ala
-
wild-type and mutant lacking residues 476-486, no substrate. Mutant lacking residues 476-486 plus mutation N275R, small D,D-carboxypeptidase activity
-
-
?
nitrocefin + H2O
?
-
-
-
-
?
additional information
?
-
(R)-2-aminobutanamide + H2O
(R)-2-aminobutanoic acid + NH3
-
-
-
-
?
(R)-2-aminobutanamide + H2O
(R)-2-aminobutanoic acid + NH3
-
-
-
-
?
D-2-aminobutyric acid amide + H2O
D-aminobutyric acid + NH3
-
-
-
-
?
D-2-aminobutyric acid amide + H2O
D-aminobutyric acid + NH3
-
-
-
-
?
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
93% of the activity with Gly 4-nitroanilide
-
-
?
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
93% of the activity with Gly 4-nitroanilide
-
-
?
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
-
-
-
?
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
-
-
?
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
-
-
-
?
D-Ala 4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
-
-
?
D-Ala-4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
-
-
?
D-Ala-4-nitroanilide + H2O
D-Ala + 4-nitroaniline
-
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala
-
-
-
-
?
D-Ala-Gly + H2O
D-Ala + Gly
-
-
-
?
D-Ala-Gly + H2O
D-Ala + Gly
-
-
-
-
?
D-Ala-Gly + H2O
D-Ala + Gly
-
-
-
-
?
D-Ala-Gly-Gly + H2O
D-Ala + Gly-Gly
-
-
-
?
D-Ala-Gly-Gly + H2O
D-Ala + Gly-Gly
-
-
-
?
D-Ala-Gly-Gly + H2O
D-Ala + Gly-Gly
-
-
-
-
?
D-alanine amide + H2O
D-Ala + NH3
-
-
-
-
?
D-alanine amide + H2O
D-Ala + NH3
-
-
-
-
?
D-serine amide + H2O
D-serine + NH3
-
-
-
-
?
D-serine amide + H2O
D-serine + NH3
-
-
-
-
?
D-serine amide + H2O
D-serine + NH3
-
-
-
-
?
Gly 4-nitroanilide + H2O
Gly + 4-nitroaniline
-
-
-
?
Gly 4-nitroanilide + H2O
Gly + 4-nitroaniline
-
-
-
-
?
Gly-L-Ala + H2O
Gly + L-Ala
-
-
-
?
Gly-L-Ala + H2O
Gly + L-Ala
-
-
-
-
?
Gly-L-Cys + H2O
Gly + L-Cys
-
-
-
?
Gly-L-Cys + H2O
Gly + L-Cys
-
-
-
-
?
L-Ala 4-nitroanilide + H2O
L-Ala + 4-nitroaniline
12% of the activity with Gly 4-nitroanilide
-
-
?
L-Ala 4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
12% of the activity with Gly 4-nitroanilide
-
-
?
additional information
?
-
enzyme shows strict D-stereospecific aminopeptidase activity. No or very poor activity with beta-Ala 4-nitroanilide, L-Leu 4-nitroanilide, D-Leu 4-nitroanilide,L-Met 4-nitroanilide, L-Pro 4-nitroanilide, L-Val 4-nitroanilide, L-Ile 4-nitroanilide, L-Phe 4-nitroanilide, D-Phe 4-nitroanilide, L-Lys 4-nitroanilide, L-Arg 4-nitroanilide, L-His 4-nitroanilide, L-Asp 4-nitroanilide, L-Glu 4-nitroanilide. No substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide
-
-
?
additional information
?
-
-
enzyme shows strict D-stereospecific aminopeptidase activity. No or very poor activity with beta-Ala 4-nitroanilide, L-Leu 4-nitroanilide, D-Leu 4-nitroanilide,L-Met 4-nitroanilide, L-Pro 4-nitroanilide, L-Val 4-nitroanilide, L-Ile 4-nitroanilide, L-Phe 4-nitroanilide, D-Phe 4-nitroanilide, L-Lys 4-nitroanilide, L-Arg 4-nitroanilide, L-His 4-nitroanilide, L-Asp 4-nitroanilide, L-Glu 4-nitroanilide. No substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide
-
-
?
additional information
?
-
substrates beta-Ala-4-nitroanilide, L-Leu-4-nitroanilide, D-Leu-4-nitroanilide, L-Met-4-nitroanilide, L-Pro-4-nitroanilide, L-Val-4-nitroanilide, L-Ile-4-nitroanilide, L-Phe-4-nitroanilide, D-Phe-4-nitroanilide, L-Lys-4-nitroanilide, L-Arg-4-nitroanilide, L-His-4-nitroanilide, L-Asp-4-nitroanilide, L-Glu-4-nitroanilide show less than 0.5% of the activity with Gly-4-nitroanilide, no substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide
-
-
?
additional information
?
-
-
substrates beta-Ala-4-nitroanilide, L-Leu-4-nitroanilide, D-Leu-4-nitroanilide, L-Met-4-nitroanilide, L-Pro-4-nitroanilide, L-Val-4-nitroanilide, L-Ile-4-nitroanilide, L-Phe-4-nitroanilide, D-Phe-4-nitroanilide, L-Lys-4-nitroanilide, L-Arg-4-nitroanilide, L-His-4-nitroanilide, L-Asp-4-nitroanilide, L-Glu-4-nitroanilide show less than 0.5% of the activity with Gly-4-nitroanilide, no substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide
-
-
?
additional information
?
-
-
strict D-stereospecificity, the enzyme prefers peptides to amino acid arylamides or amino acid amides
-
-
?
additional information
?
-
-
strict D-stereospecificity, the enzyme prefers peptides to amino acid arylamides or amino acid amides
-
-
?
additional information
?
-
-
D-valine amide, D-proline amide and D-leucine amide show less than 2% of hte activity with D-2-aminobutanamide. No activity with D-arginine amide, D-phenylalanine amide, or with L-amino acid amides
-
-
?
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Asano, Y.; Nakazawa, A.; Kato, Y.; Kondo, K.
Properties of a novel D-stereospecific aminopeptidase from Ochrobactrum anthropi
J. Biol. Chem.
264
14233-14239
1989
Brucella anthropi, Brucella anthropi SCRC C1-38
brenda
Asano, Y.; Kato, Y.; Yamada, A.; Kondo, K.
Structural similarity of D-aminopeptidase to carboxypeptidase DD and beta-lactamases
Biochemistry
31
2316-2328
1992
Brucella anthropi, Brucella anthropi SCRC C1-38
brenda
Asano, Y.; Yamaguchi, K.
Mutants of D-aminopeptidase with increased thermal stabilit
J. Ferment. Bioeng.
79
614-616
1995
Brucella anthropi
-
brenda
Asano, Y.; Yamaguchi, S.
Dynamic kinetic resolution of amino acid amide catalyzed by D-aminopeptidase and alpha-amino-epsilon-caprolactam racemase
J. Am. Chem. Soc.
127
7696-7697
2005
Brucella anthropi
brenda
Cheggour, A.; Fanuel, L.; Duez, C.; Joris, B.; Bouillenne, F.; Devreese, B.; Van Driessche, G.; Van Beeumen, J.; Frere, J.M.; Goffin, C.
The dppA gene of Bacillus subtilis encodes a new D-aminopeptidase
Mol. Microbiol.
38
504-513
2000
Bacillus subtilis (P26902), Bacillus subtilis
brenda
Delmarcelle, M.; Boursoit, M.C.; Filee, P.; Baurin, S.L.; Frere, J.M.; Joris, B.
Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis
Protein Sci.
14
2296-2303
2005
Brucella anthropi
brenda
Yamaguchi, S.; Komeda, H.; Asano, Y.
New enzymatic method of chiral amino acid synthesis by dynamic kinetic resolution of amino acid amides: use of stereoselective amino acid amidases in the presence of alpha-amino-epsilon-caprolactam racemase
Appl. Environ. Microbiol.
73
5370-5373
2007
Brucella anthropi
brenda
Yasukawa, K.; Hasemi, R.; Asano, Y.
Dynamic kinetic resolution of alpha-aminonitriles to form chiral alpha-amino acids
Adv. Synth. Catal.
353
2328-2332
2011
Brucella anthropi, Brucella anthropi C1-38
-
brenda
Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.
Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji)
Appl. Microbiol. Biotechnol.
93
655-669
2012
Aspergillus oryzae (G7H7Y1), Aspergillus oryzae
brenda
Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.
Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji)
Appl. Microbiol. Biotechnol.
93
655-669
2012
Aspergillus oryzae (G7H7Y1), Aspergillus oryzae
brenda
Tang, X.L.; Lu, X.F.; Wu, Z.M.; Zheng, R.C.; Zheng, Y.G.
Biocatalytic production of (S)-2-aminobutanamide by a novel D-aminopeptidase from Brucella sp. with high activity and enantioselectivity
J. Biotechnol.
266
20-26
2018
Brucella sp. F23/97
brenda