Cloned (Comment) | Organism |
---|---|
expression in Aspergillus oryzae | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AEBSF | 1 mM, 45% residual activity | Aspergillus oryzae | |
Cd2+ | 1 mM, less than 5% residual activity | Aspergillus oryzae | |
Co2+ | 1 mM, 50% residual activity | Aspergillus oryzae | |
Cu2+ | 1 mM, 5% residual activity | Aspergillus oryzae | |
EDTA | 1 mM, 40% residual activity | Aspergillus oryzae | |
Ni2+ | 1 mM, 20% residual activity | Aspergillus oryzae | |
Zn2+ | 1 mM, less than 5% residual activity | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
Gly 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
0.33 | - |
D-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
6.7 | - |
L-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59300 | - |
2 * 59300, calculated | Aspergillus oryzae |
126300 | - |
gel filtration | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | G7H7Y1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala 4-nitroanilide + H2O | 93% of the activity with Gly 4-nitroanilide | Aspergillus oryzae | D-Ala + 4-nitroaniline | - |
? | |
Gly 4-nitroanilide + H2O | - |
Aspergillus oryzae | Gly + 4-nitroaniline | - |
? | |
Gly-L-Ala + H2O | - |
Aspergillus oryzae | Gly + L-Ala | - |
? | |
Gly-L-Cys + H2O | - |
Aspergillus oryzae | Gly + L-Cys | - |
? | |
L-Ala 4-nitroanilide + H2O | 12% of the activity with Gly 4-nitroanilide | Aspergillus oryzae | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-D-Ala + H2O | - |
Aspergillus oryzae | L-Ala + D-Ala | - |
? | |
additional information | enzyme shows strict D-stereospecific aminopeptidase activity. No or very poor activity with beta-Ala 4-nitroanilide, L-Leu 4-nitroanilide, D-Leu 4-nitroanilide,L-Met 4-nitroanilide, L-Pro 4-nitroanilide, L-Val 4-nitroanilide, L-Ile 4-nitroanilide, L-Phe 4-nitroanilide, D-Phe 4-nitroanilide, L-Lys 4-nitroanilide, L-Arg 4-nitroanilide, L-His 4-nitroanilide, L-Asp 4-nitroanilide, L-Glu 4-nitroanilide. No substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide | Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59300, calculated | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
GdaA | - |
Aspergillus oryzae |
glycine D-alanine aminopeptidase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2100 | - |
L-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
2550 | - |
D-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
2783 | - |
Gly 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | - |
Aspergillus oryzae |
General Information | Comment | Organism |
---|---|---|
physiological function | no significant differences are observed in the vegetative growth and conidiation on minimal and complete media in the enzyme gene disruptant or overexpression strains | Aspergillus oryzae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
313 | - |
L-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
7750 | - |
D-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
11000 | - |
Gly 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae |