Application | Comment | Organism |
---|---|---|
synthesis | enantioselective hydrolysis of 2-aminobutanamide by 10 g/l recombinant cells harboring Dap results in 51.8% conversion in 10 min and 99.8% e.e, of (S)-2-aminobutanamide | Brucella sp. F23/97 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brucella sp. F23/97 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.1 | - |
D-2-aminobutanamide | pH 8.0, 35°C | Brucella sp. F23/97 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella sp. F23/97 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Brucella sp. F23/97 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-aminobutanamide + H2O | racemic, preferred substrate | Brucella sp. F23/97 | D-aminobutanoic acid + NH3 | - |
? | |
D-alanine amide + H2O | 28.3% of the activity with D-2-aminobutanamide | Brucella sp. F23/97 | D-alanine + NH3 | - |
? | |
D-serine amide + H2O | - |
Brucella sp. F23/97 | D-serine + NH3 | - |
? | |
glycine amide + H2O | 29.6% of the activity with D-2-aminobutanamide | Brucella sp. F23/97 | glycine + NH3 | - |
? | |
additional information | D-valine amide, D-proline amide and D-leucine amide show less than 2% of hte activity with D-2-aminobutanamide. No activity with D-arginine amide, D-phenylalanine amide, or with L-amino acid amides | Brucella sp. F23/97 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAP | - |
Brucella sp. F23/97 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Brucella sp. F23/97 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
2 h, less than 30% residual activity | Brucella sp. F23/97 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Brucella sp. F23/97 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | significant decrease in enzyme activity below pH 7 or above pH 9 | Brucella sp. F23/97 |