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Literature summary for 3.4.11.19 extracted from

  • Cheggour, A.; Fanuel, L.; Duez, C.; Joris, B.; Bouillenne, F.; Devreese, B.; Van Driessche, G.; Van Beeumen, J.; Frere, J.M.; Goffin, C.
    The dppA gene of Bacillus subtilis encodes a new D-aminopeptidase (2000), Mol. Microbiol., 38, 504-513.
    View publication on PubMed
Search for more literature for 3.4.11.19

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Bacillus subtilis
EDTA
-
 Bacillus subtilis
NaCl 1 M, 40% residual activity Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not activating: Fe3+, Mn2+, Cu2+, Co2+ Bacillus subtilis
Zn2+ required Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30184
-
 8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein Bacillus subtilis
30909
-
 8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein Bacillus subtilis
31000
-
 8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein Bacillus subtilis
230000
-
 gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P26902 dppA gene product
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
 pH 8.0, presence of ZnSO4 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-4-nitroanilide + H2O
-
 Bacillus subtilis D-Ala + 4-nitroaniline
-
 ?
D-Ala-D-Ala + H2O
-
 Bacillus subtilis D-Ala
-
 ?
D-Ala-Gly-Gly + H2O
-
 Bacillus subtilis D-Ala + Gly-Gly
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, stable Bacillus subtilis
60
-
 10 min, stable Bacillus subtilis
75
-
 10 min, 20% residual activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 11
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 2% of maximum activity Bacillus subtilis
7
-
 20% of maximum activity Bacillus subtilis
8
-
 60% of maximum activity Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis isoelectric focusing
-
 5
Bacillus subtilis calculated
-
 5.1