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(RS)-propan-2-yl methylphosphonofluoridate + H2O
isopropyl phosphate methylphosphonate + fluoride
i.e. sarin
-
-
?
(S)-[2-(diethylamino)ethyl] O,O-diethyl phosphorothioate + H2O
diethyl phosphate + 2-(diethylamino)ethane-1-thiol
-
-
-
?
(S)-[2-(diethylamino)ethyl] O,O-dimethyl phosphorothioate + H2O
dimethyl phosphate + 2-(diethylamino)ethane-1-thiol
-
-
-
?
(S)-[2-(diethylamino)ethyl] O-(2-methylpropyl) methylphosphonothioate + H2O
2-methylpropyl methylphosphonate + 2-(diethylamino)ethane-1-thiol
-
-
-
?
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate + H2O
diethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol
-
-
-
?
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-dimethyl phosphorothioate + H2O
dimethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol
-
-
-
?
(S)-[2-[di(propan-2-yl)amino]ethyl] O-ethyl methylphosphonothioate + H2O
ethyl methylphosphonate + [di(propan-2-yl)amino]ethane-1-thiol
-
-
-
?
2-methoxycarbonyl-1-methylvinyl dimethyl phosphate + H2O
?
i.e. mevinphos
-
-
?
3-chloro-4-methyl-2-oxo-2H-chromen-7-yl ethyl methylphosphonate + H2O
3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one + ethyl methylphosphonate
-
-
-
?
3-[fluoro(methyl)phosphoryl]oxy-2,2-dimethylbutane + H2O
Pinacolyl methylphosphonate + fluoride
i.e. soman
-
-
?
4-acetylphenyl (R)-2-methylpropyl methylphosphonate + H2O
(R)-2-methylpropyl methylphosphonate + 4-acetylphenol
-
-
-
?
4-acetylphenyl (S)-2-methylpropyl methylphosphonate + H2O
(S)-2-methylpropyl methylphosphonate + 4-acetylphenol
-
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
cyclohexylmethylphosphonofluoridate + H2O
?
i.e. cyclosarin, activity of 3.1.8.2
-
-
?
cyclohexylmethylphosphonofluoridate + H2O
cyclohexyl methylphosphonate + fluoride
i.e. cyclosarin
-
-
?
demeton-S + H2O
2-ethylsulfanyl-ethanethiol + diethyl phosphate
-
-
-
?
diethyl (3,5,6-trichloropyridin-2-yl) phosphate + H2O
?
i.e. chlorpyrifos-oxon
-
-
?
diethyl 2-(dimethoxyphosphorylsulfanyl)butanedioate + H2O
?
i.e. malaoxon
-
-
?
diethyl 4-chlorophenyl phosphate + H2O
4-chlorophenol + diethyl phosphate
-
-
-
?
diethyl 4-chlorophenyl thiophosphate + H2O
4-chlorophenol + diethyl thiophosphate
-
-
-
?
diethyl 4-methylbenzylphosphonate + H2O
?
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
diethyl-paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
dimethyl-parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
?
ethyl dimethylphosphoramidocyanidate + H2O
?
i.e. tabun
-
-
?
N,N-diethyl-2-(methyl-(2-methylpropoxy)phosphoryl)sulfanylethanamine + H2O
?
i.e. VR
-
-
?
O,O-diethyl-S-[2-diethylaminoethyl]thiophosphate + H2O
?
i.e. amiton or VG
-
-
?
O,O-diethyl-S-[2-diisopropylaminoethyl]thiophosphate + H2O
?
i.e. diisopropyl-amiton
-
-
?
O,O-diethyl-S-[2-dimethylaminoethyl]thiophosphate + H2O
?
i.e. dimethyl-amiton
-
-
?
O-ethyl S-(2-diisopropylaminoethyl)methylphosphonothioate + H2O
?
also called VX
-
-
?
O-ethyl-S-(2-di-n-propylaminoethyl)methylphosphonothiolate + H2O
?
i.e. n-propyl-VX
-
-
?
O-ethyl-S-(2-diethylaminoethyl)ethylphosphonothiolate + H2O
?
i.e. VE
-
-
?
O-ethyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VM
-
-
?
O-ethyl-S-(2-diisopropylaminoethyl)ethylphosphonothiolate + H2O
?
i.e. isopropyl-VE
-
-
?
O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VX
-
-
?
O-ethyl-S-(2-dimethylaminoethyl)ethylphosphonothiolate + H2O
?
i.e. methyl-VE
-
-
?
O-ethyl-S-(2-dimethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. dimethyl-VX
-
-
?
O-isobutyl S-(2-N,N-diethylaminoethyl)methylphosphonothioate + H2O
?
also called VR
-
-
?
O-isobutyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. VR or Russian VX
-
-
?
O-methyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. methyl-VM
-
-
?
O-n-butyl-S-(2-diethylaminoethyl)methylphosphonothiolate + H2O
?
i.e. CVX or Chinese VX
-
-
?
paraoxon + H2O
4-nitrophenol + di-ethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
paraoxon + H2O
diethyl phosphate + 4-nitrophenol
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
?
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
-
-
?
parathion + H2O
diethyl thiophosphate + 4-nitrophenol
-
-
-
?
1,2,2-trimethylpropyl methylphosphonofluoridate + H2O
3,3-dimethylbutan-2-yl methylphosphonate + HF
-
-
-
-
?
acephate + H2O
?
-
-
-
-
?
CVX + H2O
?
-
i.e. diethylamino-ethyl-O-butyl methylphosphonothioate
-
-
?
cyclosarin + H2O
methyl-phosphonic acid monofluoride + cyclohexanol
-
-
-
-
?
demethon-S + H2O
?
-
-
-
-
?
diisopropyl fluorophosphate + H2O
?
-
-
-
-
?
diisopropylfluorophosphate + H2O
diisopropyl phosphate + HF
-
-
-
-
?
dimefox + H2O
?
-
-
-
-
?
isopropyl methylphosphonofluoridate + H2O
propan-2-yl methylphosphonate + HF
-
-
-
-
?
malathion + H2O
?
-
-
-
-
?
methyl paraoxon + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
-
?
methyl parathion + H2O
4-nitrophenol + dimethyl thiophosphate
-
-
-
-
?
methyl parathion + H2O
?
-
-
-
-
?
methyl parathion + H2O
dimethyl thiophosphate + 4-nitrophenol
-
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
paraoxon + H2O
diethylphosphate + 4-nitrophenol
parathion + H2O
4-nitrophenol + diethyl thiophosphate
-
-
-
-
?
parathion + H2O
?
-
-
-
-
?
parathion + H2O
diethylthiophosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenyl thiophosphate
-
-
?
russian VX + H2O
methyl-phosphonothioic acid S-(2-diethylamino-ethyl) ester + 2-methylpropanol
-
-
-
-
?
sarin + H2O
methyl-phosphonic acid monofluoride + isopropyl alcohol
-
-
-
-
?
soman + H2O
methyl-phosphonic acid monofluoride + 1,2,2-trimethylpropanol
-
-
-
-
?
tabun + H2O
cyanophosphonic acid dimethylamide + ethanol
-
-
-
-
?
VR + H2O
?
-
i.e. diethylamino-ethyl-O-isobutyl methylphosphonothioate
-
-
?
VX + H2O
?
-
i.e. diisopropylamino-ethyl-O-ethyl methylphosphonothioate
-
-
?
VX + H2O
S-[2-(diisopropylamino)ethyl]-methylphosphonothioic acid + ethanol
-
-
-
-
?
additional information
?
-
coroxon + H2O
diethyl phosphate + chlorferon
substrate is used for in vitro activity detection
-
-
?
coroxon + H2O
diethyl phosphate + chlorferon
-
substrate is used for in vitro activity detection
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
-
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
fluorimetric detection on solid medium
-
-
?
dimethyl-paraoxon + H2O
dimethyl phosphate + 4-nitrophenol
-
fluorimetric detection on solid medium
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
the enzyme prevents, independent of compound aging, the inhibition of acetylcholinesterase by paraoxon, and also by VX, sarin and soman, the latter are phosphyloxime products of acetlycholinesterase reaction with organophosphate inhibitors, overview
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
i.e. diethyl-4-nitrophenyl phosphate
-
-
?
additional information
?
-
the enzyme is involved in detoxification of a broad range of organophosphorus pesticides and chemical warfare agents sarin and VX
-
-
?
additional information
?
-
the enzyme also exhibits activity of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview
-
-
?
additional information
?
-
-
the enzyme also exhibits activity of EC 3.1.8.2, diisopropyl-fluorophosphatase. Enzyme-substrate docking study, overview
-
-
?
additional information
?
-
wild-type PTE exhibits little stereoselectivity against the relatively small phosphonate center of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, but the elevated pKa of the thiol-leaving group provides a significant challenge for enzyme-catalyzed hydrolysis. Wild-type PTE has enzymatic activity for the hydrolysis of racemic VR similar to VX, but the larger isobutyl group attached to the phosphorus center results in a 25fold preference for the RP-enantiomer. Computational docking study of wild-type and mutant enzymes with the substrates
-
-
?
additional information
?
-
-
overview on substrate specificity
-
-
?
additional information
?
-
-
OPH catalyzes hydrolysis of organophosphorus compounds, OPC15-20, to give phosphoric or alkylphosphonic acid derivatives, which is accompanied by a decrease in the pH of the reaction medium through release of two protons
-
-
?
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0.35 - 3.2
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
0.15 - 4
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
0.03 - 4.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
1.6 - 7
diethyl 4-chlorophenyl phosphate
0.3 - 0.6
diethyl 4-chlorophenyl thiophosphate
0.34 - 1.9
diethyl 4-nitrophenyl phosphate
0.0017 - 0.081
diethyl-paraoxon
0.073 - 0.21
methylparathion
additional information
additional information
-
0.35
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
0.6
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
0.65
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
0.73
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
0.76
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
0.8
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
0.87
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant wild-type enzyme
1
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F
1.2
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
1.4
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
1.6
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
2.8
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
3.2
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
0.15
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
0.16
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
0.18
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
0.23
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
0.36
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
0.41
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
0.62
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
0.71
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
1.4
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
1.7
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant wild-type enzyme
1.7
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
2
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
2.1
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
4
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
0.03
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
0.13
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
0.18
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
0.18
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
0.19
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
0.19
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
0.2
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
0.23
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
0.24
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
0.31
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
0.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
1.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
1.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
1.7
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
4.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant wild-type enzyme
1.9
demeton-S
mutant enzyme H257L, in tripart buffer (pH 8.0)
4.2
demeton-S
mutant enzyme H254R, in tripart buffer (pH 8.0)
4.4
demeton-S
wild type enzyme, in tripart buffer (pH 8.0)
6.2
demeton-S
mutant enzyme H254R/H257L, in tripart buffer (pH 8.0)
7.6
demeton-S
mutant enzyme H254R/H257F, in tripart buffer (pH 8.0)
1.6
diethyl 4-chlorophenyl phosphate
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
3.3
diethyl 4-chlorophenyl phosphate
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
7
diethyl 4-chlorophenyl phosphate
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
0.3
diethyl 4-chlorophenyl thiophosphate
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
0.5
diethyl 4-chlorophenyl thiophosphate
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
0.6
diethyl 4-chlorophenyl thiophosphate
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
0.34
diethyl 4-nitrophenyl phosphate
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
0.74
diethyl 4-nitrophenyl phosphate
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
1.9
diethyl 4-nitrophenyl phosphate
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
0.0017
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
0.0036
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
0.0053
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
0.0053
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant H254Q/H257F
0.0054
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
0.0056
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
0.0067
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
0.0072
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
0.008
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
0.0092
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N
0.0092
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
0.011
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F
0.021
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
0.022
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
0.025
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
0.033
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
0.081
diethyl-paraoxon
pH 9.0, 30°C, recombinant wild-type enzyme
0.012
paraoxon
mutant H254R, pH 9.0, 25°C
0.035
paraoxon
wild-type enzyme
0.05
paraoxon
mutant enzyme H254R/H257F, in 20 mM CHES buffer (pH 9.0) at 25°C
0.07
paraoxon
mutant enzyme H254R/H257L, in 20 mM CHES buffer (pH 9.0) at 25°C
0.09
paraoxon
mutant enzyme H254R, in 20 mM CHES buffer (pH 9.0) at 25°C
0.1
paraoxon
wild type enzyme, in 20 mM CHES buffer (pH 9.0) at 25°C
0.12
paraoxon
wild-type enzyme, pH 9.0, 25°C
0.26
paraoxon
Zn2+-PTE, at pH 8.5 and 30°C
0.3
paraoxon
mutant enzyme H257L, in 20 mM CHES buffer (pH 9.0) at 25°C
0.34
paraoxon
Co2+-PTE mutant H123N, at pH 8.5 and 30°C
0.36
paraoxon
Co2+-PTE mutant H123I, at pH 8.5 and 30°C
0.39
paraoxon
Co2+-PTE mutant H123A, at pH 8.5 and 30°C
0.4
paraoxon
Co2+-PTE, at pH 8.5 and 30°C
0.6
parathion
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
1.5
parathion
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
1.7
parathion
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
0.073
methylparathion
-
pH 10.5, 20°C, recombinant His6-tagged OPH
0.21
methylparathion
-
pH 9.0, 20°C, native OPH
0.01
paraoxon
-
pH 10.5, 20°C, recombinant His6-tagged OPH
0.01
paraoxon
-
pH 10.5, 25°C, recombinant His6-tagged wild-type OPH
0.016
paraoxon
-
pH 9.0, 20°C, native OPH
0.104
paraoxon
-
pH 10.5, 25°C, 3-fluorotyrosine-containing recombinant His6-tagged OPH
0.29
paraoxon
-
native enzyme, in 50 mM borate buffer (pH 8.5, 0.1 mM CoCl2), at 25°C
0.015
parathion
-
pH 10.5, 20°C, recombinant His6-tagged OPH
0.06
parathion
-
pH 9.0, 20°C, native OPH
additional information
additional information
kinetic evaluation, mutant H254R
-
additional information
additional information
kinetics of the Zn-enzyme and the Cd-enzyme, wild-type and mutant, kinetic model, overview
-
additional information
additional information
-
kinetics of the Zn-enzyme and the Cd-enzyme, wild-type and mutant, kinetic model, overview
-
additional information
additional information
classical Michaelis-Menten kinetics
-
additional information
additional information
reaction kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
reaction kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
Km values of paraoxon hydrolysis with different metal ions in the active site
-
additional information
additional information
-
kinetics of recombinant His6-tagged wild-type OPH and 3-fluorotyrosine-containing recombinant His6-tagged OPH
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.1 - 51
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
17 - 174
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
3 - 166
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
0.13 - 0.36
diethyl 4-chlorophenyl phosphate
1.1 - 2.4
diethyl 4-chlorophenyl thiophosphate
2060 - 2500
diethyl 4-nitrophenyl phosphate
15 - 2230
diethyl-paraoxon
5
1,2,2-trimethylpropyl methylphosphonofluoridate
-
-
41
diisopropylfluorophosphate
-
-
56
isopropylmethylphosphonofluoridate
-
-
1.1
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant wild-type enzyme
3 - 6
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
5.1
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
6.1
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
8.6
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
13
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
14
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
16
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
19
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F
22
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
23
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
31
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
51
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
17
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
45
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
46
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
50
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
55
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
57
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
58
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
72
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
84
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant wild-type enzyme
100
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
101
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
122
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
160
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
174
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
3 - 6
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
8.1
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
8.7
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
14
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
21
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
25
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant wild-type enzyme
29
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
33
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
40
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
51
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
53
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
56
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
159
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
166
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
3.3
demeton-S
mutant enzyme H257L, in tripart buffer (pH 8.0)
3.5
demeton-S
wild type enzyme, in tripart buffer (pH 8.0)
32
demeton-S
mutant enzyme H254R, in tripart buffer (pH 8.0)
34
demeton-S
mutant enzyme H254R/H257F, in tripart buffer (pH 8.0)
50
demeton-S
mutant enzyme H254R/H257L, in tripart buffer (pH 8.0)
0.13
diethyl 4-chlorophenyl phosphate
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
0.23
diethyl 4-chlorophenyl phosphate
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
0.36
diethyl 4-chlorophenyl phosphate
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
1.1
diethyl 4-chlorophenyl thiophosphate
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
2.2
diethyl 4-chlorophenyl thiophosphate
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
2.4
diethyl 4-chlorophenyl thiophosphate
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
2060
diethyl 4-nitrophenyl phosphate
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
2300
diethyl 4-nitrophenyl phosphate
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
2500
diethyl 4-nitrophenyl phosphate
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
15
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
33
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
35
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
38
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
41
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant H254Q/H257F
48
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
58
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N
66
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
85
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
103
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
108
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F
116
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
131
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
446
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
456
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
545
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
2230
diethyl-paraoxon
pH 9.0, 30°C, recombinant wild-type enzyme
50
paraoxon
Co2+-PTE mutant H123I, at pH 8.5 and 30°C
390
paraoxon
Co2+-PTE mutant H123A, at pH 8.5 and 30°C
450
paraoxon
mutant enzyme H254R/H257F, in 20 mM CHES buffer (pH 9.0) at 25°C
640
paraoxon
mutant enzyme H254R/H257L, in 20 mM CHES buffer (pH 9.0) at 25°C
750
paraoxon
mutant H254R, pH 9.0, 25°C
780
paraoxon
pH 8.5, recombinant wild-type enzyme-MBP fusion protein
1200
paraoxon
mutant enzyme H254R, in 20 mM CHES buffer (pH 9.0) at 25°C
1280
paraoxon
pH 8.5, recombinant genetic variants 1.5E-MBP fusion protein
1290
paraoxon
Zn2+-PTE, at pH 8.5 and 30°C
1430
paraoxon
pH 8.5, recombinant genetic variants 1.6F-MBP fusion protein
1610
paraoxon
Co2+-PTE mutant H123N, at pH 8.5 and 30°C
2150
paraoxon
pH 8.5, recombinant genetic variants 2.S5-MBP fusion protein
2280
paraoxon
wild-type enzyme
2280
paraoxon
pH 9.0, recombinant wild-type enzyme
4010
paraoxon
Co2+-PTE, at pH 8.5 and 30°C
6900
paraoxon
wild type enzyme, in 20 mM CHES buffer (pH 9.0) at 25°C
8000
paraoxon
mutant enzyme H257L, in 20 mM CHES buffer (pH 9.0) at 25°C
10500
paraoxon
wild-type enzyme, pH 9.0, 25°C
300
parathion
recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C
600
parathion
recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C
720
parathion
recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C
125
paraoxon
-
pH 10.5, 25°C, 3-fluorotyrosine-containing recombinant His6-tagged OPH
3400
paraoxon
-
pH 8.0, 30°C
5100
paraoxon
-
pH 10.5, 25°C, recombinant His6-tagged wild-type OPH
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.264 - 85
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
21.4 - 333.3
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
5.55 - 883.3
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
1920 - 27531
diethyl-paraoxon
1.264
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant wild-type enzyme
4.0625
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
4.357
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
5.375
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
11.071
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
14.571
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
19
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F
21.053
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
21.539
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
28.75
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
30
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
30.137
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
85
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
21.4
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
25
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
32.7
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
34.375
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
49.4
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant wild-type enzyme
61
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
81.7
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
94.1
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
124.3
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
158.3
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
162.9
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
255.6
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
313
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
333.3
4-acetylphenyl (R)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
5.55
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant wild-type enzyme
9.3
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
17.1
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
34
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
38.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
42.6
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
110.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
112
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
143.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
166.67
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
180
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
290
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant H254Q/H257F
294.4
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
535.5
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
883.3
4-acetylphenyl (S)-2-methylpropyl methylphosphonate
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
1920
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F
4925
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F
6304
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N
6786
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L
7736
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant H254Q/H257F
8824
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N
9722
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
9818
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F
11806
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
12222
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L
14239
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
14500
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
16515
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
19434
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
20727
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L
21239
diethyl-paraoxon
pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
27531
diethyl-paraoxon
pH 9.0, 30°C, recombinant wild-type enzyme
140
paraoxon
Co2+-PTE mutant H123I, at pH 8.5 and 30°C
1000
paraoxon
Co2+-PTE mutant H123A, at pH 8.5 and 30°C
4700
paraoxon
Co2+-PTE mutant H123N, at pH 8.5 and 30°C
5000
paraoxon
Zn2+-PTE, at pH 8.5 and 30°C
10000
paraoxon
Co2+-PTE, at pH 8.5 and 30°C
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A204C/T234C
site-directed mutagenesis, Ala204 and Thr234 are located on the alpha-helices and gamma turn, respectively, thermostability of the mutant enzyme is increased compared to the wild-type, due to increased rigidity
A80V/F132V/K185R/H254Q/H257Y/I274N
site-directed mutagenesis
A80V/F132V/K185R/H254Q/H257Y/I274N/S308L
site-directed mutagenesis, the mutant combines expression-enhancing mutations (A80V/K185R/I274N) with additional changes in the active site to achieve a high kcat/Km for the hydrolysis of the SP-enantiomer of ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX. Three-dimensional structure, overview
A80V/I106C/F132V/K185R/H254Q/H257Y/I274N
site-directed mutagenesis
A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L
site-directed mutagenesis
A80V/I106G/F132V/K185R/H254Q/H257Y/I274N
site-directed mutagenesis
A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L
site-directed mutagenesis
C24S
site-directed mutagenesis, mutation of the invariant cysteine residue in the lipobox motif of the OPH signal peptide
D233A
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
D233N
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
D301A
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
D301N
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
F132V/H254Q/H257F
site-directed mutagenesis
F132V/H254Q/H257F/S308L
site-directed mutagenesis
G74C/A78C
site-directed mutagenesis, Gly74 and Ala78 are located on the gamma turn and beta-turn, respectively, which link gamma turn and beta-turn adjacent together, thermostability of the mutant enzyme is decreased compared to the wild-type, due to increased flexibility
H123A
KM remains unaffected, whereas kcat decreases 2.5fold. Alters the bacterial expression yield of Co2+-PTE and Ki for Zn(OH)2 inhibition
H123I
KM remains unaffected, whereas kcat decreases 10fold. Alters the bacterial expression yield of Co2+-PTE and Ki for Zn(OH)2 inhibition
H123N
KM remains unaffected, whereas kcat decreases 80fold. Alters the bacterial expression yield of Co2+-PTE and Ki for Zn(OH)2 inhibition
H254A
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
H254N
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
H254Q/H257F
site-directed mutagenesis, the mutant exhibits a 100fold improvement for the hydrolysis of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, relative to the wild-type enzyme
H254R/H257F
the mutant enzyme shows reduced activity towards paraoxon and increased activity towards demeton-S compared to the wild type enzyme
H254R/H257L
the variant has higher turnover numbers for the chemical warfare agents O-ethyl S-diisopropyl aminomethyl methylphosphonothioate and O-isobutyl S-N,N-diethylaminoethyl methylphosphonothioate compared to the wild type enzyme, the demeton-S activity is over 14times higher as that of the wild type enzyme
H257L
the mutant enzyme shows increased activity towards paraoxon and reduced activity toward demeton-S compared to the wild type enzyme
I106C
site-directed mutagenesis
I106C/F132V/H254Q/H257F
site-directed mutagenesis
I106C/F132V/H254Q/H257F/S308L
site-directed mutagenesis, the mutant shows a similar catalytic efficiency for the hydrolysis of ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, as mutant H254Q/H257F, but a 3fold improvement in kcat
I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
site-directed mutagenesis, the mutant has a kcat value for ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, that is enhanced by 150fold relative to wild-type PTE. Three-dimensional structure, overview
I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F
site-directed mutagenesis
I106G
site-directed mutagenesis
I106G/F132V/H254Q/H257F/S308L
site-directed mutagenesis
I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L
site-directed mutagenesis
L308S
site-directed mutagenesis
T128C/ E124C
site-directed mutagenesis, thermostability and kcat of the mutant enzyme are increased compared to the wild-type
T128C/ E153C
site-directed mutagenesis, Thr128 and Glu153 are located on the beta-sheet and alpha-helices, respectively, thermostability of the mutant enzyme is increased compared to the wild-type, due to increased rigidity
I106A/F132A/H254Y
-
stereoselectivity of enzyme toward chiral phosphorous center of substrate is reversed
H254R
site-directed mutagenesis, the mutation increases the mutants activity with substrate demeton-S, a VX analogue, 4fold and decreases the activity with paraoxon 14fold as well as activity towards diisopropyl phosphorofluoridate, a sarin analogue, 183fold compared to the wild-type enzyme
H254R
the mutant enzyme is not completely denatured by urea concentrations up to 8.5 M even after incubation for 24 h, the mutant is more stable than the wild type enzyme and shows increased activity with demeton-S 9times over that of the wild type enzyme
Y309F
site-directed mutagenesis
Y309F
site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview
additional information
a PTE mutant dubbed C23 is engineered, exhibiting reversed stereoselectivity and high catalytic efficiency (kcat/KM) for the hydrolysis of the toxic enantiomers of nerve agents VX, CVX, and VR. The other mutants A53, IV-A1, IV-H3, B141, and RD1-G83 are less effective, activities and mutant-substrate docking, overview. The only exception is IV-A1 with amiton. Most variants are highly efficient when hydrolyzing N,N-diisopropyl substrates and were 2fold less efficient with N,N-di-ethyl substrates and 5fold less efficient with N,N-di-methyl substrates
additional information
-
a PTE mutant dubbed C23 is engineered, exhibiting reversed stereoselectivity and high catalytic efficiency (kcat/KM) for the hydrolysis of the toxic enantiomers of nerve agents VX, CVX, and VR. The other mutants A53, IV-A1, IV-H3, B141, and RD1-G83 are less effective, activities and mutant-substrate docking, overview. The only exception is IV-A1 with amiton. Most variants are highly efficient when hydrolyzing N,N-diisopropyl substrates and were 2fold less efficient with N,N-di-ethyl substrates and 5fold less efficient with N,N-di-methyl substrates
additional information
appropriate amino acid pairs for the introduction of disulfide bridge to improve protein thermostability are selected, engineering of de novo disulfide bridges is explored as a means to increase the thermal stability of enzymes in the rational method of protein engineering. Circular dichroism (CD) spectrometry and intrinsic fluorescence experiments are applied to evaluate the structural changes of mutants compared to the wild-type after introducing disulfide bridge
additional information
computational docking study of wild-type and mutant enzymes with the toxic substrates
additional information
generation of opd null mutants, strain DS010
additional information
-
generation of opd null mutants, strain DS010
additional information
-
construction of a 3-fluorotyrosine-containing enzyme by replacement of Tyr residues, Tyr292, Tyr239, and Tyr248 residues are completely replaced by the fluorinated analogue, while the Tyr156 residue is replaced by 90%, overview, the substitution extends substantially the pH range of action of the fluorine-substituted enzyme compared with the unsubstituted one, the mutant shows increased pH and temperature stability, but reduced activity
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Dumas, D.P.; Durst, H.D.; Landis, W.G.; Raushel, F.M.; Wild, J.R.
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