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IUBMB Comments The enzyme, which has been characterized from the bacterium Mycobacterium tuberculosis , has no activity with O -acetyl-L -serine. Requires pyridoxal 5′-phosphate. cf . EC 2.5.1.144 , S -sulfo-L -cysteine synthase (O -acetyl-L -serine-dependent).
Synonyms CysK2 , O-phosphoserine S-sulfocysteine synthase,
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O-phosphoserine S-sulfocysteine synthase
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CysK2
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3-phospho-L-serine + thiosulfate = S-sulfo-L-cysteine + phosphate
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thiosulfate:3-phospho-L-serine thiosulfotransferase
The enzyme, which has been characterized from the bacterium Mycobacterium tuberculosis, has no activity with O-acetyl-L-serine. Requires pyridoxal 5'-phosphate. cf. EC 2.5.1.144, S-sulfo-L-cysteine synthase (O-acetyl-L-serine-dependent).
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3-phospho-L-serine + sulfide
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Substrates: -
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3-phospho-L-serine + thiosulfate
S-sulfo-L-cysteine + phosphate
additional information
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Substrates: the enzyme does not utilize thiocarboxylated CysO as a sulfur donor. O-acetyl-L-serine, L-Asp, L-Val, L-Gln, L-Glu, L-Ser, L-Asn, L-Cys, L-Ser, L-Leu, homocysteine, pyruvate, 2-oxoglutarate, 3-phosphoglycerate, succinate, N-acetylcysteine and diaminopimelic acid are not used as substrate
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3-phospho-L-serine + thiosulfate
S-sulfo-L-cysteine + phosphate
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Substrates: -
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3-phospho-L-serine + thiosulfate
S-sulfo-L-cysteine + phosphate
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Substrates: highest activity
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3-phospho-L-serine + thiosulfate
S-sulfo-L-cysteine + phosphate
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Substrates: -
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pyridoxal 5'-phosphate
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2-[[(2,5-dimethoxyphenyl)carbamoyl]amino]benzoic acid
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3-[(3',4'-dichloro[1,1'-biphenyl]-3-sulfonyl)amino]benzoic acid
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3-[[(3,4-dichlorophenyl)carbamoyl]amino]benzoic acid
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3-[[(4-bromophenyl)carbamoyl]amino]benzoic acid
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3-[[(4-chlorophenyl)carbamoyl]amino]benzoic acid
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3-[[(4-methylphenyl)carbamoyl]amino]benzoic acid
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3-[[([1,1'-biphenyl]-3-yl)carbamoyl]amino]benzoic acid
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4-[[(4-bromophenyl)carbamoyl]amino]-2-hydroxybenzoic acid
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4-[[([1,1'-biphenyl]-3-yl)carbamoyl]amino]-2-hydroxybenzoic acid
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5-[[(3,4-dichlorophenyl)carbamoyl]amino]-2-hydroxybenzoic acid
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5-[[([1,1'-biphenyl]-3-yl)carbamoyl]amino]-2-hydroxybenzoic acid
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N-(3,4-dichlorophenyl)-N'-[3-(1H-tetrazol-5-yl)phenyl]urea
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0.4851 - 1.0855
3-phospho-L-serine
0.0439 - 0.2137
thiosulfate
0.4851
3-phospho-L-serine
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mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
1.0855
3-phospho-L-serine
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wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
0.0439
thiosulfate
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wild type enzyme, at pH 9.0 and 22°C
0.2137
thiosulfate
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mutant enzyme R243A, at pH 9.0 and 22°C
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0.457 - 0.913
3-phospho-L-serine
0.457 - 0.913
thiosulfate
0.457
3-phospho-L-serine
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mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
0.913
3-phospho-L-serine
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wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
0.457
thiosulfate
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mutant enzyme R243A, at pH 9.0 and 22°C
0.913
thiosulfate
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wild type enzyme, at pH 9.0 and 22°C
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0.000083 - 0.000093
3-phospho-L-serine
0.0021 - 0.0208
thiosulfate
0.000083
3-phospho-L-serine
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wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
0.000093
3-phospho-L-serine
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mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
0.0021
thiosulfate
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mutant enzyme R243A, at pH 9.0 and 22°C
0.0208
thiosulfate
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wild type enzyme, at pH 9.0 and 22°C
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0.0101
3-[[(3,4-dichlorophenyl)carbamoyl]amino]benzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
0.0276
3-[[(4-bromophenyl)carbamoyl]amino]benzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
0.013
3-[[(4-chlorophenyl)carbamoyl]amino]benzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
0.0066
3-[[([1,1'-biphenyl]-3-yl)carbamoyl]amino]benzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
0.0269
4-[[([1,1'-biphenyl]-3-yl)carbamoyl]amino]-2-hydroxybenzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
0.0299
5-[[(3,4-dichlorophenyl)carbamoyl]amino]-2-hydroxybenzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
0.0103
5-[[([1,1'-biphenyl]-3-yl)carbamoyl]amino]-2-hydroxybenzoic acid
Mycobacterium tuberculosis
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pH and temperature not specified in the publication
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Highest Expressing Human Cell Lines
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CYSK2_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
372
0
40118
Swiss-Prot
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R243A
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the mutant shows about 10% activity with sulfide compared to the wild type enzyme
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Ni-NTA column chromatography and Superdex 200 gel filtration
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expressed in Escherichia coli BL21(DE3) cells
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Brunner, K.; Steiner, E.M.; Reshma, R.S.; Sriram, D.; Schnell, R.; Schneider, G.
Profiling of in vitro activities of urea-based inhibitors against cysteine synthases from Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
27
4582-4587
2017
Mycobacterium tuberculosis
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Steiner, E.M.; Boeth, D.; Loessl, P.; Vilaplana, F.; Schnell, R.; Schneider, G.
CysK2 from Mycobacterium tuberculosis is an O-phospho-L-serine-dependent S-sulfocysteine synthase
J. Bacteriol.
196
3410-3420
2014
Mycobacterium tuberculosis
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