BRENDA - Enzyme Database
show all sequences of 2.8.5.1

CysK2 from Mycobacterium tuberculosis is an O-phospho-L-serine-dependent S-sulfocysteine synthase

Steiner, E.M.; Boeth, D.; Loessl, P.; Vilaplana, F.; Schnell, R.; Schneider, G.; J. Bacteriol. 196, 3410-3420 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Engineering
Amino acid exchange
Commentary
Organism
R243A
the mutant shows about 10% activity with sulfide compared to the wild type enzyme
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0439
-
thiosulfate
wild type enzyme, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.2137
-
thiosulfate
mutant enzyme R243A, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.4851
-
3-phospho-L-serine
mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
1.0855
-
3-phospho-L-serine
wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-phospho-L-serine + thiosulfate
Mycobacterium tuberculosis
-
S-sulfo-L-cysteine + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-phospho-L-serine + sulfide
-
748092
Mycobacterium tuberculosis
?
-
-
-
?
3-phospho-L-serine + thiosulfate
-
748092
Mycobacterium tuberculosis
S-sulfo-L-cysteine + phosphate
-
-
-
?
3-phospho-L-serine + thiosulfate
highest activity
748092
Mycobacterium tuberculosis
S-sulfo-L-cysteine + phosphate
-
-
-
?
additional information
the enzyme does not utilize thiocarboxylated CysO as a sulfur donor. O-acetyl-L-serine, L-Asp, L-Val, L-Gln, L-Glu, L-Ser, L-Asn, L-Cys, L-Ser, L-Leu, homocysteine, pyruvate, 2-oxoglutarate, 3-phosphoglycerate, succinate, N-acetylcysteine and diaminopimelic acid are not used as substrate
748092
Mycobacterium tuberculosis
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.457
-
3-phospho-L-serine
mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.457
-
thiosulfate
mutant enzyme R243A, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.913
-
3-phospho-L-serine
wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.913
-
thiosulfate
wild type enzyme, at pH 9.0 and 22°C
Mycobacterium tuberculosis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Mycobacterium tuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R243A
the mutant shows about 10% activity with sulfide compared to the wild type enzyme
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0439
-
thiosulfate
wild type enzyme, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.2137
-
thiosulfate
mutant enzyme R243A, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.4851
-
3-phospho-L-serine
mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
1.0855
-
3-phospho-L-serine
wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-phospho-L-serine + thiosulfate
Mycobacterium tuberculosis
-
S-sulfo-L-cysteine + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-phospho-L-serine + sulfide
-
748092
Mycobacterium tuberculosis
?
-
-
-
?
3-phospho-L-serine + thiosulfate
-
748092
Mycobacterium tuberculosis
S-sulfo-L-cysteine + phosphate
-
-
-
?
3-phospho-L-serine + thiosulfate
highest activity
748092
Mycobacterium tuberculosis
S-sulfo-L-cysteine + phosphate
-
-
-
?
additional information
the enzyme does not utilize thiocarboxylated CysO as a sulfur donor. O-acetyl-L-serine, L-Asp, L-Val, L-Gln, L-Glu, L-Ser, L-Asn, L-Cys, L-Ser, L-Leu, homocysteine, pyruvate, 2-oxoglutarate, 3-phosphoglycerate, succinate, N-acetylcysteine and diaminopimelic acid are not used as substrate
748092
Mycobacterium tuberculosis
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.457
-
3-phospho-L-serine
mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.457
-
thiosulfate
mutant enzyme R243A, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.913
-
3-phospho-L-serine
wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.913
-
thiosulfate
wild type enzyme, at pH 9.0 and 22°C
Mycobacterium tuberculosis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Mycobacterium tuberculosis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.000083
-
3-phospho-L-serine
wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.000093
-
3-phospho-L-serine
mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.0021
-
thiosulfate
mutant enzyme R243A, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.0208
-
thiosulfate
wild type enzyme, at pH 9.0 and 22°C
Mycobacterium tuberculosis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.000083
-
3-phospho-L-serine
wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.000093
-
3-phospho-L-serine
mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.0021
-
thiosulfate
mutant enzyme R243A, at pH 9.0 and 22°C
Mycobacterium tuberculosis
0.0208
-
thiosulfate
wild type enzyme, at pH 9.0 and 22°C
Mycobacterium tuberculosis
Other publictions for EC 2.8.5.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747281
Brunner
Profiling of in vitro activit ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
27
4582-4587
2017
-
-
-
-
-
-
12
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
1
-
-
-
7
12
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748092
Steiner
CysK2 from Mycobacterium tube ...
Mycobacterium tuberculosis
J. Bacteriol.
196
3410-3420
2014
-
-
1
-
1
-
-
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
4
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
4
1
-
-
-
-
-
-
-
4
4