Ligand thiosulfate

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of thiosulfate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H2O3S2
thiosulfate
DHCDFWKWKRSZHF-UHFFFAOYSA-L
Synonyms:
H2S2O3, S2O3-, S2O32-, thiosulphate


Show all pahtways known for Show all BRENDA pathways known for thiosulfate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (27 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
show the reaction diagram
-
2 thiosulfate + ubiquinone = tetrathionate + ubiquinol
show the reaction diagram
-
O-acetyl-L-serine + thiosulfate = S-sulfo-L-cysteine + acetate
show the reaction diagram
-
O-acetyl-L-Ser + S2O32- = S-sulfocysteine + ?
show the reaction diagram
-
O-phospho-L-serine + thiosulfate = S-sulfocysteine + phosphate
show the reaction diagram
-
thiosulfate + cyanide = SO32- + thiocyanate
show the reaction diagram
-
thiosulfate + glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
thiosulfate + 2 dithioerythritol = sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
-
3-phospho-L-serine + thiosulfate = S-sulfo-L-cysteine + phosphate
show the reaction diagram
-
ATP + H2O + thiosulfate/out = ADP + phosphate + thiosulfate/in
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5 sulfur + 2 O2 + 2 H2O + 4 H+ = sulfite + thiosulfate + 2 sulfide
show the reaction diagram
-
-
tetrathionate + a reduced electron acceptor = 2 thiosulfate + an oxidized electron acceptor
show the reaction diagram
-
-
3-mercaptopyruvate + HSO3- = pyruvate + S2O32-
show the reaction diagram
-
-
ATP + H2O + thiosulfate/out = ADP + phosphate + thiosulfate/in
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (88 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
thiosulfate + NADH + H+ = ? + NAD+
show the reaction diagram
-
thiosulfate + menaquinol = sulfite + hydrogen sulfide + menaquinone
show the reaction diagram
-
O-phospho-L-serine + thiosulfate = S-sulfocysteine + phosphate
show the reaction diagram
-
3-phospho-L-serine + thiosulfate = S-sulfo-L-cysteine + phosphate
show the reaction diagram
-
horse heart cytochrome c + thiosulfate = ?
show the reaction diagram
-
ATP + H2O + thiosulfate/out = ADP + phosphate + thiosulfate/in
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (24 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
tetrathionate + reduced methylene blue = thiosulfate + oxidized methylene blue
show the reaction diagram
-
-
sulfite + hydrogen sulfide + menaquinone = thiosulfate + menaquinol
show the reaction diagram
-
-
hydrogen sulfide + sulfite + coenzyme Q1 = thiosulfate + reduced coenzyme Q1
show the reaction diagram
-
-
tetrathionate + a reduced electron acceptor = 2 thiosulfate + an oxidized electron acceptor
show the reaction diagram
-
-
thiosulfate + sulfite = sulfite + thiosulfate
show the reaction diagram
-
-
ATP + H2O + thiosulfate/out = ADP + phosphate + thiosulfate/in
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
greater reactivation at 33 mM than at 3.3 mM
-
activates SO42dependent reaction in presence of 0.15 mM of 3'-phosphoadenosine-5'-phosphate
2.2fold induction
-

Inhibitor in Enzyme-catalyzed Reactions (28 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
above 0.5 mM
-
product inhibition, 40% inhibition at 1 mM
-
1 mM, 15% loss of activity
-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
-

Metals and Ions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
stimulates
-

3D Structure of Enzyme-Ligand-Complex (PDB) (21 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (27 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.33
-
cosubstrate NADH, pH 7.4, 30C
167
-
; 80C, pH 6.0
11.3
-
pH 8.4, 30C

KM Value (88 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.112
-
cosubstrate NADH, pH 7.4, 30C
0.5
-
37C, pH 7.6
0.81
-
-

Ki Value (4 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

Links to other databases for thiosulfate

ChEBI
PubChem
ChEBI
PubChem