Information on EC 2.8.3.20 - succinyl-CoA-D-citramalate CoA-transferase

for references in articles please use BRENDA:EC2.8.3.20
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The expected taxonomic range for this enzyme is: Chloroflexus aurantiacus

EC NUMBER
COMMENTARY hide
2.8.3.20
-
RECOMMENDED NAME
GeneOntology No.
succinyl-CoA-D-citramalate CoA-transferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA
show the reaction diagram
succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
itaconate degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:(R)-citramalate CoA-transferase
The enzyme, purified from the bacterium Clostridium tetanomorphum, can also accept itaconate as acceptor, with lower efficiency.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + (R)-citramalate
succinate + (R)-citramalyl-CoA
show the reaction diagram
succinyl-CoA + (R)-malate
succinate + (R)-malyl-CoA
show the reaction diagram
succinyl-CoA + itaconate
succinate + ?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + (R)-citramalate
succinate + (R)-citramalyl-CoA
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity is not dependent on Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
(R)-citramalate
-
55C, pH and temperature not specified in the publication
0.08
succinyl-CoA
-
55C, pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13
(R)-citramalate
-
55C, pH and temperature not specified in the publication
13
succinyl-CoA
-
55C, pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17.5
-
55C, pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
2 * 44000, SDS-PAGE
90000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity
-
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heterologously expressed in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
upregulated under autotrophic conditions