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Literature summary for 2.8.3.20 extracted from

  • Friedmann, S.; Alber, B.E.; Fuchs, G.
    Properties of succinyl-coenzyme A:D-citramalate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus (2006), J. Bacteriol., 188, 6460-6468.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.20

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Chloroflexus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 succinyl-CoA 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus
2.2
-
 (R)-citramalate 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is not dependent on Mg2+ Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, SDS-PAGE Chloroflexus aurantiacus
90000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + (R)-citramalate Chloroflexus aurantiacus the enzyme catalyzes a steps in the glyoxylate assimilation route succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate Chloroflexus aurantiacus DSM 636 the enzyme catalyzes a steps in the glyoxylate assimilation route succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate Chloroflexus aurantiacus OK-70-fl / DSM 636 the enzyme catalyzes a steps in the glyoxylate assimilation route succinate + (R)-citramalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
-
-
Chloroflexus aurantiacus DSM 636
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Chloroflexus aurantiacus

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + (R)-citramalate = succinate + (R)-citramalyl-CoA (1) Chloroflexus aurantiacus
a
succinyl-CoA + (R)-malate = succinate + (R)-malyl-CoA (2) Chloroflexus aurantiacus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.5
-
 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus

Storage Stability

Storage Stability Organism
4°C, pH 7, 1 week, in the presence of 100 mM KCl, without significant loss of activity Chloroflexus aurantiacus
frozen for months in the presence of 10% glycerol (v/v), without significant loss of activity Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + (R)-citramalate
-
 Chloroflexus aurantiacus succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate
-
 Chloroflexus aurantiacus DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate
-
 Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-citramalate the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + (R)-citramalyl-CoA
-
 ?
succinyl-CoA + (R)-malate 88% compared to the activity with (R)-citramalate Chloroflexus aurantiacus succinate + (R)-malyl-CoA
-
 ?
succinyl-CoA + (R)-malate 88% compared to the activity with (R)-citramalate Chloroflexus aurantiacus DSM 636 succinate + (R)-malyl-CoA
-
 ?
succinyl-CoA + (R)-malate 88% compared to the activity with (R)-citramalate Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + (R)-malyl-CoA
-
 ?
succinyl-CoA + itaconate 80% compared to the activity with (R)-citramalate Chloroflexus aurantiacus succinate + ?
-
 ?
succinyl-CoA + itaconate 80% compared to the activity with (R)-citramalate Chloroflexus aurantiacus DSM 636 succinate + ?
-
 ?
succinyl-CoA + itaconate 80% compared to the activity with (R)-citramalate Chloroflexus aurantiacus OK-70-fl / DSM 636 succinate + ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 44000, SDS-PAGE Chloroflexus aurantiacus

Synonyms

Synonyms Comment Organism
SCT
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Chloroflexus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
 succinyl-CoA 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus
13
-
 (R)-citramalate 55°C, pH and temperature not specified in the publication Chloroflexus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 at 55°C Chloroflexus aurantiacus

Expression

Organism Comment Expression
Chloroflexus aurantiacus upregulated under autotrophic conditions up

General Information

General Information Comment Organism
physiological function the enzyme catalyzes a steps in the glyoxylate assimilation route Chloroflexus aurantiacus